LOCUS       sequence-308833-       11770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11770)
  AUTHORS   Solomon DA, Kim JS, Cronin JC, Sibenaller Z, Ryken T, Rosenberg SA, 
            Ressom H, Jean W, Bigner D, Yan H, Samuels Y, Waldman T
  TITLE     Mutational inactivation of PTPRD in glioblastoma multiforme and 
            malignant melanoma.
  JOURNAL   Cancer Res. 2008 Dec 15. 68(24):10300-6.
  PUBMED    19074898
REFERENCE   2  (bases 1 to 11770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2008 Dec 15. 68(24):10300-6."
COMMENT     Sequence Label: pCDF1-PTPRD-WT
FEATURES             Location/Qualifiers
     source          1..11770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        133..336
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        1479..1682
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1679..1703
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        7009..7220
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             7233..7501
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             7528..8127
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7528..7547)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8024..8044
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8134..8722
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8187..8207)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8605..8616)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9059..9180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9096..9115)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9150..9169
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9199..9334
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9367..9383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9367..9383)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9380..9402)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9391..9407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9415..9421,9422..9439,9440..9445))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9460..9481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9598..9615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9769..10357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9849..9868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10528..11319,11320..11388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11151..11170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11389..11493)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11561..11579
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11617..11639)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11739..11758
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
       61 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      121 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      181 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      241 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      301 aggcgtgtac ggtgggaggt ctatataagc agagcttgtg aaacttcgag gagtctcttt
      361 gttgaggact tttgagttct cccttgaggc tcccacagat acaataaata tttgagattg
      421 aaccctgtcg agtatctgtg taatcttttt tacctgtgag gtctcggaat ccgggccgag
      481 aacttcgcag ttggcgcccg aacagggact tgattgagag tgattgagga agtgaagcta
      541 gagcaataga aagctgttaa gcagaactcc tgctgaccta aatagggaag cagtagcaga
      601 cgctgctaac agtgagtatc tctagtgaag cagactcgag ctcataatca agtcattgtt
      661 taaaggccca gataaattac atctggtgac tcttcgcgga ccttcaagcc aggagattcg
      721 ccgagggaca gtcaacaagg taggagagat tctacagcaa catggggaat ggacaggggc
      781 gagattggaa aatggccatt aagagatgta gtaatgttgc tgtaggagta ggggggaaga
      841 gtaaaaaatt tggagaaggg aatttcagat gggccattag aatggctaat gtatctacag
      901 gacgagaacc tggtgatata ccagagactt tagatcaact aaggttggtt atttgcgatt
      961 tacaagaaag aagagaaaaa tttggatcta gcaaagaaat tgatatggca attcctgcat
     1021 tgaggagaaa tggtaggcaa tgtggcatgt ctgaaaaaga ggaggaatga tgaagtatct
     1081 cagacttatt ttataaggga gatactgtgc tgagttcttc cctttgagga aggtatgtca
     1141 tatcctagac atagtctcaa ttttaaaaga agaggtagga taggagggat ggccccttat
     1201 gaattattag cacaacaaga atccttaaga atacaagatt atttttctgc aataccacaa
     1261 aaattgcaag cacagtggat ttattataaa gatcaaaaag ataagaaatg gaaaggacca
     1321 atgagagtag aatactgggg acagggatca gtattattaa aggatgaaga gaagggatat
     1381 tttcttataa tcgatactag tattatgccc agtacatgac cttatgggac tttcctactt
     1441 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     1501 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     1561 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     1621 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     1681 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     1741 gaagattcta gagcccgggc gcgccggatc cagatcttaa tgcatgctcg agcggccgcc
     1801 agtgtgatgg atatctgcag aattcgccct tcaccccgga ggcagtgatt ccaagctgct
     1861 cgcgcacgct gctgccaagc tgcaggatgg tgcacgtagc caggctgctg ctgctgctcc
     1921 tcactttctt cctccgcacg gatgctgaga cacctccaag gtttacacga acacccgttg
     1981 atcagacagg ggtctctggc ggagttgcct ctttcatctg ccaagctacg ggagacccaa
     2041 gacctaaaat tgtctggaac aaaaaaggaa agaaagtcag caatcagaga tttgaggtaa
     2101 tagagtttga cgatgggtct ggatcagttc tcagaataca acccttacgg actccgaggg
     2161 atgaggccat ttatgaatgt gtggcctcaa ataatgtggg agaaataagt gtatccacca
     2221 gactcacagt tttgcgggaa gatcaaattc ccaggggctt ccctaccatt gacatgggcc
     2281 cacagttgaa ggtggttgag cgtactcgca cggccaccat gctttgtgca gccagtggta
     2341 atccggatcc agaaatcact tggtttaaag atttcttacc tgtggacaca agcaacaaca
     2401 atggtcgtat taagcagtta cgatcagaat ctattggtgg tacaccaata agaggagccc
     2461 ttcagattga gcagagtgaa gagtctgacc aaggaaaata tgagtgtgtt gccaccaaca
     2521 gcgcgggcac tcgctattcc gctcctgcca atttatatgt cagagagctg cgagaagttc
     2581 gccgtgtccc accaagattc tctatcccac ccactaatca tgaaatcatg ccaggcggaa
     2641 gcgttaatat cacctgtgtg gccgtggggt caccaatgcc ttatgtaaag tggatgttgg
     2701 gggcagaaga tctgacacct gaagatgata tgccaatagg aagaaatgtg ctagaactga
     2761 atgatgtaag acagtcagca aattacacct gtgttgctat gtcaacactg ggtgtcattg
     2821 aagcaatagc acagatcact gtcaaagcct tacccaaacc tccaggaact cctgtagtga
     2881 ccgagagcac agctacaagc atcacactga cgtgggactc tgggaaccct gagcctgttt
     2941 cttattacat aattcagcat aaacctaaaa actctgagga actttacaaa gaaattgatg
     3001 gggtggcgac cacacgctac agtgtcgctg gactaagtcc ctactcggat tatgaattca
     3061 gggttgttgc tgtcaataac attgggcggg ggcctcccag cgaacctgtg ctaacacaaa
     3121 cctcagagca agcaccatcc agtgccccga gggatgtcca ggcacgaatg ttgagttcga
     3181 ccaccatttt ggtacagtgg aaggaacctg aagagccaaa tggacagatc caaggatata
     3241 gagtttatta tacaatggat cccactcaac atgtcaacaa ctggatgaaa cacaatgtag
     3301 ctgacagcca aatcactact attggcaact tagtgcccca gaaaacatat tctgtcaaag
     3361 tcctggcttt tacctcaatt ggagatggtc ccctttcaag tgacatacaa gtcatcactc
     3421 agacaggagt accagggcag ccactaaact tcaaagcaga acctgagtct gaaacaagta
     3481 ttttgctctc ttggacacct ccacgttcag ataccattgc caactatgaa ctggtctaca
     3541 aagatgggga gcatggagag gagcaacgaa ttaccattga gccagggaca tcatataggc
     3601 tgcaaggact gaaaccaaac agcttatact atttccgtct ggctgcacgc tcccctcaag
     3661 gcctgggtgc ttctactgca gaaatatcag ctagaaccat gcagtcaatg tttgcaaaaa
     3721 attttcatgt caaagcagta atgaagactt ccgtgttgct gtcttgggag attccagaga
     3781 attataactc cgccatgcct ttcaaaattc tttatgatga tgggaaaatg gtagaagaag
     3841 tggatggccg agccacacag aagttaattg tcaacctgaa gcctgagaaa tcatattcat
     3901 ttgtgctgac aaatcgtgga aacagtgctg gtgggctgca gcacagggtc acggcaaaga
     3961 ctgcaccaga tgtattacgt accaagcctg ccttcattgg gaagaccaac ttggatggca
     4021 tgattactgt gcaactgcct gaagtacctg caaatgagaa tataaaaggt tactacataa
     4081 taattgtgcc tttgaagaaa tctcgcggga aatttatcaa gccatgggag agtccagatg
     4141 aaatggaatt agatgagctg cttaaggaga tatctaggaa gcgcagaagc atccgttatg
     4201 ggagagaagt tgaattaaag ccatatattg ccgctcactt tgatgtcctt cccactgagt
     4261 tcaccctggg ggatgacaag cattatggtg gatttacaaa caagcaactc caaagtggtc
     4321 aagaatatgt cttctttgtg ttagcagtaa tggaacatgc agagtctaag atgtatgcaa
     4381 ccagccctta ctccgacccc gtggtgtcaa tggatctgga tccgcagcca atcacggatg
     4441 aagaagaagg cttgatctgg gttgtaggtc ctgtccttgc agtggtcttt atcatctgca
     4501 ttgtcattgc tattcttctt tataaaagca gtaaacccga caggaagagg gcagagtccg
     4561 actctagaaa aagcagcata ccgaacaata aggagatccc ttcacaccac ccaacagacc
     4621 ctgtagaact gaggcgcctt aactttcaaa caccgggtat ggctagccat cctccaatac
     4681 ccatcttgga acttgcagac cacattgaaa gattgaaagc aaatgacaac ttgaagtttt
     4741 cccaggaata tgagtcaatt gaccctggcc agcagttcac ttgggaacat tcaaacttgg
     4801 aagtaaacaa accaaagaat agatacgcga atgtaatcgc atatgatcat tcccgggttc
     4861 tcctatcagc tatagaaggg atcccaggaa gtgactatgt gaatgccaac tacatagatg
     4921 ggtataggaa gcaaaatgcc tatattgcaa cacagggatc tctccccgaa acatttgggg
     4981 acttttggag aatgatatgg gaacaacgga gtgccacagt tgtcatgatg acaaaactag
     5041 aagaaagatc aagggtgaag tgtgaccagt attggcctag cagaggcaca gaaacccacg
     5101 gactcgttca agtaacgctg cttgatactg tggagctggc cacatattgt gttcgaacat
     5161 ttgcacttta caagaatggt tcaagtgaga agagagaagt gagacaattc cagttcaccg
     5221 cctggcctga tcatggtgtt ccagaacacc ctacaccttt tctagctttc ttacgtagag
     5281 tcaaaacctg taaccctccc gatgctggtc cgatggttgt gcactgcagt gcgggagttg
     5341 gccggactgg ttgcttcatc gtcatagatg ccatgttaga aagaataaag catgaaaaaa
     5401 ctgtagatat ttatggccat gtaactttaa tgagagccca gaggaactat atggttcaaa
     5461 cagaagacca atacatcttt atccatgatg cactgttaga agcagtgact tgtggaaata
     5521 ccgaagtgcc agctagaaac ttgtatgcct acattcagaa gctgacacaa atagaaacgg
     5581 gagagaatgt cacaggaatg gagctcgaat ttaagcgtct agccagctca aaagctcaca
     5641 cctcaaggtt tatcagtgcc aatcttccat gtaataaatt caaaaatcgc cttgttaata
     5701 ttatgccata tgaatccaca agggtatgcc tgcagcctat ccgtggagta gaaggatctg
     5761 attacatcaa tgccagtttt attgatggat acagacaaca gaaagcctac atcgctaccc
     5821 aggggccctt ggcagagacc actgaagact tctggcggat gctctgggaa cacaattcca
     5881 ccatagttgt gatgctcacc aagctgcgtg aaatgggcag agagaaatgt caccaatact
     5941 ggccagcaga acggtctgca agataccagt actttgttgt agatcccatg gctgagtaca
     6001 acatgccaca gtatatccta agggaattca aggtcacaga tgccagggac ggccagtccc
     6061 gaacagtaag gcagttccag ttcactgact ggccagagca aggagtgcca aagtccggag
     6121 aaggatttat tgacttcatc ggccaagtcc ataaaacaaa agaacagttt ggccaagatg
     6181 gacccatttc agtccattgc agcgcgggcg ttggaagaac tggagtcttc ataacgctaa
     6241 gcattgtttt ggaaagaatg agatatgaag gagttgtaga tatcttccag actgtcaaaa
     6301 tgttaagaac acaacgacca gctatggtac agacagagga tcaatatcag ttttcctatc
     6361 gtgccgcact agagtacctg ggcagctttg accactatgc aacgtagaaa cccctgaccc
     6421 attctggatt tttactacag gcccttcaat atccatggag tctcttctga gccatacagg
     6481 gcacttgaga agtccttctt aacttctagc taacaactac ttagtgggac tattacacac
     6541 aaaacaaatt aaaaacaaat tattccaggt ggaccaagaa ttcacagttt aataatctaa
     6601 cctgaaaaat aatgaagaga atcctgactg atgaggcatc tgaaggattt tatttacaga
     6661 tacctcaagg attcaaaatc aagggaagaa gaaatcacag caaagaacca ccatcttatt
     6721 caggattgct tgaaaggtgc aaggagaaat tgccagaatg ctgcagttca gcacaagata
     6781 tgtgctggtg tggcttctca caatgaaacg gactctgctt tgacatcgcc ccttcccacc
     6841 atactgctca taataacatt ttaggggcca aggggaataa tataaagggc gaattccagc
     6901 acactggcgg ccgttactag tggatccgag ctcggtacca agcttgatgc ataatttaaa
     6961 tgaattcgcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
     7021 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
     7081 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     7141 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     7201 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
     7261 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
     7321 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
     7381 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
     7441 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
     7501 ccaagctgtg accggcgcct acgctagatg accgagtaca agcccacggt gcgcctcgcc
     7561 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     7621 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     7681 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     7741 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     7801 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     7861 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     7921 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7981 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     8041 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     8101 tgcatgaccc gcaagcccgg tgcctgagtc gacaatcaac ctctggatta caaaatttgt
     8161 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8221 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8281 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8341 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8401 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     8461 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8521 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     8581 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8641 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     8701 tccctttggg ccgcctcccc gcctggtacc gatgacagag ttagaagatc gcttcaggaa
     8761 gctatttggc acgacttcta caacgggaga cagcacagta gattctgaag atgaacctcc
     8821 taaaaaagaa aaaagggtgg actgggatga gtattggaac cctgaaatcg atagcttcca
     8881 gtgctttgtg aaacttcgag gagtctcttt gttgaggact tttgagttct cccttgaggc
     8941 tcccacagat acaataaata tttgagattg aaccctgtcg agtatctgtg taatcttttt
     9001 tacctgtgag gtctcggaat ccgggccgag aacttcgcag cgagctcatt gtaccgcgaa
     9061 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9121 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9181 tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc
     9241 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9301 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
     9361 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9421 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9481 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9541 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9601 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9661 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9961 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10201 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10381 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10441 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10501 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10561 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10621 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10681 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10741 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10801 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10861 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10921 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10981 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11041 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    11101 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11161 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11221 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11281 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11341 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11401 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11461 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11521 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    11581 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    11641 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    11701 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    11761 gagagtgcac
//
