LOCUS       sequence-308843-       10925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9B(-10TC)_arr2
FEATURES             Location/Qualifiers
     source          1..10925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(9..23,24..29,30..46,47..53)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    29..45
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    61..77
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             96..107
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(114..140,141..143,144..2630,2631..2633,2634..4220)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     rep_origin      4914..5502
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5403..5422
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagaaaatc ataaaaaatt tatttgcttt gtgagcggat aacaattata acagattcaa
       61 ttgtgagcgg ataacaattt cacacagaat tcattaaaga ggagaaatta catatggata
      121 agaaatactc aataggctta gctatcggca caaatagcgt cggatgggcg gtgatcactg
      181 atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac cgccacagta
      241 tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg gaagcgactc
      301 gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt tgttatctac
      361 aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat cgacttgaag
      421 agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt ggaaatatag
      481 tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga aaaaaattgg
      541 tagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg catatgatta
      601 agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt gatgtggaca
      661 aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac cctattaacg
      721 caagtggagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca agacgattag
      781 aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cttatttggg aatctcattg
      841 ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca gaagatgcta
      901 aattacagct ttcaaaagat acttacgatg atgatttaga taatttattg gcgcaaattg
      961 gagatcaata tgctgatttg tttttggcag ctaagaattt atcagatgct attttacttt
     1021 cagatatcct aagagtaaat actgaaataa ctaaggctcc cctatcagct tcaatgatta
     1081 aacgctacga tgaacatcat caagacttga ctcttttaaa agctttagtt cgacaacaac
     1141 ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat gcaggttata
     1201 ttgatggggg agctagccaa gaagaatttt ataaatttat caaaccaatt ttagaaaaaa
     1261 tggatggtac tgaggaatta ttggtgaaac taaatcgtga agatttgctg cgcaagcaac
     1321 ggacctttga caacggctct attccccatc aaattcactt gggtgagctg catgctattt
     1381 tgagaagaca agaagacttt tatccatttt taaaagacaa tcgtgagaag attgaaaaaa
     1441 tcttgacttt tcgaattcct tattatgttg gtccattggc gcgtggcaat agtcgttttg
     1501 catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg
     1561 ataaaggtgc ttcagctcaa tcatttattg aacgcatgac aaactttgat aaaaatcttc
     1621 caaatgaaaa agtactacca aaacatagtt tgctttatga gtattttacg gtttataacg
     1681 aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa accagcattt ctttcaggtg
     1741 aacagaagaa agccattgtt gatttactct tcaaaacaaa tcgaaaagta accgttaagc
     1801 aattaaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa atttcaggag
     1861 ttgaagatag atttaatgct tcattaggta cctaccatga tttgctaaaa attattaaag
     1921 ataaagattt tttggataat gaagaaaatg aagatatctt agaggatatt gttttaacat
     1981 tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat gctcacctct
     2041 ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg ggacgtttgt
     2101 ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata ttagattttt
     2161 tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat gatagtttga
     2221 catttaaaga agacattcaa aaagcacaag tgtctggaca aggcgatagt ttacatgaac
     2281 atattgcaaa tttagctggt agccctgcta ttaaaaaagg tattttacag actgtaaaag
     2341 ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc gttattgaaa
     2401 tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac
     2461 gaatcgaaga aggtatcaaa gaattaggaa gtcagattct taaagagcat cctgttgaaa
     2521 atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga agagacatgt
     2581 atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat gccattgttc
     2641 cacaaagttt ccttaaagac gattcaatag acaataaggt cttaacgcgt tctgataaaa
     2701 atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg aaaaactatt
     2761 ggagacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat ttaacgaaag
     2821 ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc caattggttg
     2881 aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg aatactaaat
     2941 acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa tctaaattag
     3001 tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac aattaccatc
     3061 atgcccatga tgcgtatcta aatgccgtcg ttggaactgc tttgattaag aaatatccaa
     3121 aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt aaaatgattg
     3181 ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac tctaatatca
     3241 tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa cgccctctaa
     3301 tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat tttgccacag
     3361 tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa gtccagacag
     3421 gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt attgctcgta
     3481 aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta gcttattcag
     3541 tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt
     3601 tactagggat cacaattctg gaaagaagtt cctttgaaaa aaatccgatt gactttttag
     3661 aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct aaatatagtc
     3721 tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa ttacaaaaag
     3781 gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct agtcattatg
     3841 aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg gagcagcata
     3901 agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt gttattttag
     3961 cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac aaaccaatac
     4021 gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga gctcccgctg
     4081 cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca aaagaagttt
     4141 tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc attgatttga
     4201 gtcagctagg aggtgactaa taaaagcttg atcagatccc agcttttttt tgaagtgcct
     4261 ggcagtaagt tgaaaaaatt tgaaataaac ttggactatg ttaataaaaa aatacaaatt
     4321 aacgaacaaa aagatttgaa ttattatata atttagttaa aagcaatttt aaatgaggga
     4381 ggtttccatc atatggctaa acctttaagt caagaagaaa gtactttaat tgaaagagct
     4441 actgctacta ttaatagtat tcctattagt gaagattata gtgttgctag tgctgcttta
     4501 agtagtgatg gtagaatttt tactggtgtt aatgtttatc attttactgg tggtccttgt
     4561 gctgaattag ttgttttagg tactgccgcc gctgctgctg ctggtaatct cacttgtatt
     4621 gttgctattg gtaatgaaaa tagaggtatt ttaagtcctt gtggtagatg tagacaagtt
     4681 ttattagatt tacatcctgg tattaaagct attgttaaag atagtgatgg tcaacctact
     4741 gctgttggta ttagagaatt attacctagt ggttatgttt gggaaggtta atgacgtcgc
     4801 cggagcggtc gagttctgga ccgaccggct cgggttctca tgaccaaaat cccttaacgt
     4861 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4921 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4981 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5041 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5101 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5161 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5221 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5281 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5341 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5401 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5461 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5521 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcactagat
     5581 ccccggcaag ttcatcctta gggttttcat atctcttaaa aagtcaattt ctgcttgatt
     5641 aatttcttgt ggattattgt ttttgcggtt tttcattttt tatctccttg agtattgaat
     5701 tttttcttat atataaatat atagcaaaaa ctatttttgc caactttttt acaaaaaatt
     5761 ttttattaaa acacttaggg ctttgctaaa ttctctttta aaagaactta gtaaagccct
     5821 atggatttaa gaactgatta tacttacgta gtaaaaatac tacagatatt aatagtatag
     5881 accatatcaa aatcataatc aatttatatt tatctaaaat tgtagtccat acaaaataca
     5941 aaaaaattag gtttttctaa attctatttt aaaagaactt agtaacacct aatctctaaa
     6001 ttacttacaa aattctaaaa ttagattttt agttcttcat atttctttaa aagtttttta
     6061 agaaaatctt tttgattttc aaaaatttca tgcatcataa aactagtaaa tttggatttt
     6121 tttttgtaaa aatcataact ttcttgggtt ttaagttgaa atcttaatgg ttttattgga
     6181 ttttgcctag atttttttaa cgccggactt tctttatctt ttataaaatt ataagaattt
     6241 ttaataccat tttctattaa ataatcttct tttataagcc cctcttgcag tgcagttgct
     6301 aatcttaaat acttatacgc ttgagttttt gctacatcaa agtcttttat aaaatcaaga
     6361 aaagttttat aaccatcaaa tttataatat tgattatctt ttatttcttt taaaatttta
     6421 atattttcca ccctgtggaa tatttctttt ttgaaattgt catttaactt ttcctttagt
     6481 gtcttgtaat aagaaatttt actttcatta atcattaaat tttgttcaac ataagtatct
     6541 atcttttttg ttaaaacttt aacaacacgt tttttttcca taaaaatttt cctttatttt
     6601 tgcaacattt tccttaattg atataattgt tactatttta gattttttaa tatccactta
     6661 agaattaatc cctcactaga atatttttta ttatagaatt catatacatg tttttctaaa
     6721 tctataaatt tatcaacaat tgctttatta tagggtgtat ctatcttttc ttttcttaat
     6781 aatcgtctca tattctttaa ataacaacaa acactgccgg ttttaaacct aaattctatg
     6841 taatatattt ttgaaaaagt atatacattt tcaaccccgt ttatttcata ctttacaaaa
     6901 acattattta ttggttttct ataaccatag taaatgccta aaaatttgtc cccgtctttc
     6961 ataggatata gataaagttt ttgaatctct gatttatcta ataatttttc aaaaaaaatt
     7021 atacaccgct tttgtttgtt actaactact ctatgcaatg cctttaatat ttttgtgtga
     7081 tatatatttt tatcaccttt tttttctttc agcaggaaga tactttcttt tggaaattta
     7141 attttttgtt catttattct tgcagcctta agtccgattt tcatatttaa tccttatata
     7201 gcctttctat ttaattcttt taaactcaat aaactttttt ggtttttcat tagttctaat
     7261 aattcgcaat aatatgtgca aaacacttta ttgtattcta ttttcttttg gttattcaaa
     7321 tattctttta taattggctt tagaagttca atatttactt ctttttttag ttgttcaata
     7381 aggatattga aaatgtttgt ttttaaatct tgataatttt gttgagaatt ttcttttttt
     7441 ctttcaattg acttctctaa tttacgcttt atattgttta aatcgctata tttatgactt
     7501 tcaataataa aatgaggctt aaatttgtaa ttttcgtata ttttttggaa acttgtttct
     7561 aattgtttag aattatattc atttttttca aattcttttt gagtgttgca tagaatttcc
     7621 tttaattttt cttgtttttc tttcatacaa gatttagtaa aatgtatatt tttatttttt
     7681 agtaatttaa tttctattat ttttatgatt ttaagtattt tgattatatt atctttatca
     7741 atatttaaat tgaataaaat tgtaagaaaa attgaaagaa tttttttaca taaaaagtta
     7801 catttattga agtaatttct tacttgatac ttttctattt cattaatctt tctttcttct
     7861 tttatattat ttttattatt taaacactcc tttaaatcta cactcccatt tttagtaaat
     7921 ctatctttaa ggccccttga aactctattt tgaaatctta aatctttttt ttcttgaaag
     7981 tatttataga ttttttggta acattctttt tttgaaaaat taagttgata gtatatttcg
     8041 gtccccaaat ttacccctaa gtgtttgtga taattagttg tgactttaaa ttctttttct
     8101 aatttgtaaa ggtaattttg cagtgttttt agtttaacgg gtgtttgact atttctttta
     8161 aggttttcat taaagtaata gagtatgttt ttttgtgtgt atttcttata tgttttattt
     8221 atgtacgcta gtgttgagat aaggactact aatttatact gtaatttatt gtagcaagtt
     8281 gtggttttac tggttttgtt tatattagat aagttttcca tttatagctc cttgaatacc
     8341 ggatttttta tctataaagt atatatatca caattttgat ttaaaagtaa attctttttt
     8401 taaaaataca aaaatttttt ataaattata tagaccatga tattgtatta atactaattg
     8461 attttaattt aaggttgaac taaaataaac atagttttgt agaaaataat ttttcattat
     8521 tttgctactt gatattggat cgtaaaaata ttagggcttt acaaagttct tttaaaagag
     8581 aatttagtaa agccctaagt cttttaataa aaaatttttt gtaaaaaagt tggcaaaaat
     8641 agtttttgct atatatttat atataagaaa aaattcaata ctcaaggaga taaaaaatgg
     8701 aaaacttaaa caataatcca caaaaaatta atcaaataaa taagcaaact aattttattg
     8761 gttttgaaaa ttttatagaa cttgataatc aagttaatca aactaaagaa aaaagtaaat
     8821 taagtaagat aggtaagaaa ttacccggaa taagcagtca agtctagtgc ggccgcatgg
     8881 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     8941 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     9001 gtcgccctaa aacaaagtta aacatcatga ggaggtaacg tcatggtaaa agattggatt
     9061 cctatttctc atgataatta taaacaagta caaggaccat tttatcatgg aactaaagct
     9121 aatttagcaa ttggtgattt actaactaca ggatttattt ctcattttga agatggtcga
     9181 attcttaaac atatttattt ttcagcttta atggaaccag cagtttgggg agctgaactt
     9241 gctatgtcac tatctggtct tgaaggtcgt ggttatatat atatagttga accaacagga
     9301 ccatttgaag atgatccaaa tcttacaaat aaaaaatttc ctggtaatcc aacacaatct
     9361 tatagaactt gtgaaccttt aagaattgtt ggtgttgttg aagattggga aggacatcct
     9421 gttgaattaa taagaggaat gttagattca ttagaagatt taaaacgtcg tggtttacat
     9481 gttattgaag attaacctgg cgcgccaaat aatgcccgtg tccctgttga atagggacac
     9541 ggttttttaa caattttttt attcctaggc cttagtggcc ggtacccggg gatccttatt
     9601 actggccgct ttctagcctg gatacttgtc tggctagttg cattagagag tcagctagag
     9661 ccctagggct agctgtttgt gtattcggag ccagtgtagt ttttcttttt actaacgata
     9721 cgggtaataa ttgattgcct tttacagcct gaccttgaga caattgcaaa agtctgtcca
     9781 cagaggtttg acctaataat ctaaaatctt gtttgatagt agtaagtgga ggtatgtagc
     9841 aactagaatc ttctgtatca tcataaccca caactgaaat atcagcccca actctaagtc
     9901 cactttcagt aatagctcgc atagctccta aagccatttg atcattagca actaacatag
     9961 cagtagggac aatgccttca tttaacattt gcattgtttg ctgaaatcct gacatagcag
    10021 accaatctcc ttctctttct gctattggtt gtatttgatt tcttgttaaa tacttgtgcc
    10081 atcctgctaa tctcaatctg gcacttactg aacttaaggg acctgcaagt agtgcaattt
    10141 gttgatggcc taatgcaaca aggtgttcaa ctcctaacct tgttccatct tcgtgagaaa
    10201 aaattataga gtttattggg gtttggtccg acacatccaa aaacaatgct ggaacattag
    10261 tacatgctgc ttcaactgca atcgcatctt ggtcatcaag aggataatta attataagtc
    10321 cactaacacg ttgagctaat aaattatgta ctgctgcctt acaagcctct acacctgatc
    10381 tctcaaccat tgatacaaca acgcttgcac caagttgatc agctctcgat ttaattgcgg
    10441 ctacaatttg actaggcgca tgtaatgcaa gtgatgatgt ggcaactcca attaaaagtg
    10501 attgtttccc cgcaagttgc tgagcaacgc gatttggtat ataattcaac tctgccatag
    10561 ctgcttcaac cttctctctt gtttttgcag atacatgact tgcttggttc acaactctcg
    10621 aaaccgtttg gtagctaacc ccggcatatt cagcaacatc atacaaagta acaggtttca
    10681 tggatcattc ctccatgata aaatttaaat ttctgacttt ggcaaatcct tttgcctgtt
    10741 aaaaagaata gcacaaaaat taaaaaaaaa gaaacgcaaa tagcaatagc attaaaaata
    10801 attattatta tttatttctt tgattaaaat caaaagcctt taatgtattt tcttgatctt
    10861 tcatgcgctt aacggtaaaa tccaaggtat taagttcacc ttccactttt ctctctctat
    10921 cttct
//
