LOCUS       sequence-308845-       11561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11561)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9H_arr2
FEATURES             Location/Qualifiers
     source          1..11561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(2794..2808,2809..2814,2815..2831,2832..2838)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2814..2830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2846..2862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2881..2892
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2899..2925,2926..2928,2929..5415,5416..5418,
                     5419..7005)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7193..8212
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     rep_origin      8335..8923
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8824..8843
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgtttgact atttctttta aggttttcat taaagtaata gagtatgttt ttttgtgtgt
       61 atttcttata tgttttattt atgtacgcta gtgttgagat aaggactact aatttatact
      121 gtaatttatt gtagcaagtt gtggttttac tggttttgtt tatattagat aagttttcca
      181 tttatagctc cttgaatacc ggatttttta tctataaagt atatatatca caattttgat
      241 ttaaaagtaa attctttttt taaaaataca aaaatttttt ataaattata tagaccatga
      301 tattgtatta atactaattg attttaattt aaggttgaac taaaataaac atagttttgt
      361 agaaaataat ttttcattat tttgctactt gatattggat cgtaaaaata ttagggcttt
      421 acaaagttct tttaaaagag aatttagtaa agccctaagt cttttaataa aaaatttttt
      481 gtaaaaaagt tggcaaaaat agtttttgct atatatttat atataagaaa aaattcaata
      541 ctcaaggaga taaaaaatgg aaaacttaaa caataatcca caaaaaatta atcaaataaa
      601 taagcaaact aattttattg gttttgaaaa ttttatagaa cttgataatc aagttaatca
      661 aactaaagaa aaaagtaaat taagtaagat aggtaagaaa ttacccggaa taagcagtca
      721 agtctagtgc ggccgcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
      781 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
      841 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga ggaggtaacg
      901 tcatggtaaa agattggatt cctatttctc atgataatta taaacaagta caaggaccat
      961 tttatcatgg aactaaagct aatttagcaa ttggtgattt actaactaca ggatttattt
     1021 ctcattttga agatggtcga attcttaaac atatttattt ttcagcttta atggaaccag
     1081 cagtttgggg agctgaactt gctatgtcac tatctggtct tgaaggtcgt ggttatatat
     1141 atatagttga accaacagga ccatttgaag atgatccaaa tcttacaaat aaaaaatttc
     1201 ctggtaatcc aacacaatct tatagaactt gtgaaccttt aagaattgtt ggtgttgttg
     1261 aagattggga aggacatcct gttgaattaa taagaggaat gttagattca ttagaagatt
     1321 taaaacgtcg tggtttacat gttattgaag attaacctgg cgcgccaaat aatgcccgtg
     1381 tccctgttga atagggacac ggttttttaa caattttttt attcctaggc cttagtggcc
     1441 ggtacccggg gatccttatt actggccgct ttctagcctg gatacttgtc tggctagttg
     1501 cattagagag tcagctagag ccctagggct agctgtttgt gtattcggag ccagtgtagt
     1561 ttttcttttt actaacgata cgggtaataa ttgattgcct tttacagcct gaccttgaga
     1621 caattgcaaa agtctgtcca cagaggtttg acctaataat ctaaaatctt gtttgatagt
     1681 agtaagtgga ggtatgtagc aactagaatc ttctgtatca tcataaccca caactgaaat
     1741 atcagcccca actctaagtc cactttcagt aatagctcgc atagctccta aagccatttg
     1801 atcattagca actaacatag cagtagggac aatgccttca tttaacattt gcattgtttg
     1861 ctgaaatcct gacatagcag accaatctcc ttctctttct gctattggtt gtatttgatt
     1921 tcttgttaaa tacttgtgcc atcctgctaa tctcaatctg gcacttactg aacttaaggg
     1981 acctgcaagt agtgcaattt gttgatggcc taatgcaaca aggtgttcaa ctcctaacct
     2041 tgttccatct tcgtgagaaa aaattataga gtttattggg gtttggtccg acacatccaa
     2101 aaacaatgct ggaacattag tacatgctgc ttcaactgca atcgcatctt ggtcatcaag
     2161 aggataatta attataagtc cactaacacg ttgagctaat aaattatgta ctgctgcctt
     2221 acaagcctct acacctgatc tctcaaccat tgatacaaca acgcttgcac caagttgatc
     2281 agctctcgat ttaattgcgg ctacaatttg actaggcgca tgtaatgcaa gtgatgatgt
     2341 ggcaactcca attaaaagtg attgtttccc cgcaagttgc tgagcaacgc gatttggtat
     2401 ataattcaac tctgccatag ctgcttcaac cttctctctt gtttttgcag atacatgact
     2461 tgcttggttc acaactctcg aaaccgtttg gtagctaacc ccggcatatt cagcaacatc
     2521 atacaaagta acaggtttca tggatcattc ctccatgata aaatttaaat ttctgacttt
     2581 ggcaaatcct tttgcctgtt aaaaagaata gcacaaaaat taaaaaaaaa gaaacgcaaa
     2641 tagcaatagc attaaaaata attattatta tttatttctt tgattaaaat caaaagcctt
     2701 taatgtattt tcttgatctt tcatgcgctt aacggtaaaa tccaaggtat taagttcacc
     2761 ttccactttt ctctctctat cttctctaga aaatcataaa aaatttattt gctttgtgag
     2821 cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac agaattcatt
     2881 aaagaggaga aattacatat ggataagaaa tactcaatag gcttagctat cggcacaaat
     2941 agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt
     3001 ctgggaaata cagaccgcca cagtatcaaa aaaaatctta taggggctct tttatttgac
     3061 agtggagaga cagcggaagc gactcgtctc aaacggacag ctcgtagaag gtatacacgt
     3121 cggaagaatc gtatttgtta tctacaggag attttttcaa atgagatggc gaaagtagat
     3181 gatagtttct ttcatcgact tgaagagtct tttttggtgg aagaagacaa gaagcatgaa
     3241 cgtcatccta tttttggaaa tatagtagat gaagttgctt atcatgagaa atatccaact
     3301 atctatcatc tgcgaaaaaa attggtagat tctactgata aagcggattt gcgcttaatc
     3361 tatttggcct tagcgcatat gattaagttt cgtggtcatt ttttgattga gggagattta
     3421 aatcctgata atagtgatgt ggacaaacta tttatccagt tggtacaaac ctacaatcaa
     3481 ttatttgaag aaaaccctat taacgcaagt ggagtagatg ctaaagcgat tctttctgca
     3541 cgattgagta aatcaagacg attagaaaat ctcattgctc agctccccgg tgagaagaaa
     3601 aatggcttat ttgggaatct cattgctttg tcattgggtt tgacccctaa ttttaaatca
     3661 aattttgatt tggcagaaga tgctaaatta cagctttcaa aagatactta cgatgatgat
     3721 ttagataatt tattggcgca aattggagat caatatgctg atttgttttt ggcagctaag
     3781 aatttatcag atgctatttt actttcagat atcctaagag taaatactga aataactaag
     3841 gctcccctat cagcttcaat gattaaacgc tacgatgaac atcatcaaga cttgactctt
     3901 ttaaaagctt tagttcgaca acaacttcca gaaaagtata aagaaatctt ttttgatcaa
     3961 tcaaaaaacg gatatgcagg ttatattgat gggggagcta gccaagaaga attttataaa
     4021 tttatcaaac caattttaga aaaaatggat ggtactgagg aattattggt gaaactaaat
     4081 cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg gctctattcc ccatcaaatt
     4141 cacttgggtg agctgcatgc tattttgaga agacaagaag acttttatcc atttttaaaa
     4201 gacaatcgtg agaagattga aaaaatcttg acttttcgaa ttccttatta tgttggtcca
     4261 ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga agtctgaaga aacaattacc
     4321 ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag ctcaatcatt tattgaacgc
     4381 atgacaaact ttgataaaaa tcttccaaat gaaaaagtac taccaaaaca tagtttgctt
     4441 tatgagtatt ttacggttta taacgaattg acaaaggtca aatatgttac tgaaggaatg
     4501 cgaaaaccag catttctttc aggtgaacag aagaaagcca ttgttgattt actcttcaaa
     4561 acaaatcgaa aagtaaccgt taagcaatta aaagaagatt atttcaaaaa aatagaatgt
     4621 tttgatagtg ttgaaatttc aggagttgaa gatagattta atgcttcatt aggtacctac
     4681 catgatttgc taaaaattat taaagataaa gattttttgg ataatgaaga aaatgaagat
     4741 atcttagagg atattgtttt aacattgacc ttatttgaag atagggagat gattgaggaa
     4801 agacttaaaa catatgctca cctctttgat gataaggtga tgaaacagct taaacgtcgc
     4861 cgttatactg gttggggacg tttgtctcga aaattgatta atggtattag ggataagcaa
     4921 tctggcaaaa caatattaga ttttttgaaa tcagatggtt ttgccaatcg caattttatg
     4981 cagctgatcc atgatgatag tttgacattt aaagaagaca ttcaaaaagc acaagtgtct
     5041 ggacaaggcg atagtttaca tgaacatatt gcaaatttag ctggtagccc tgctattaaa
     5101 aaaggtattt tacagactgt aaaagttgtt gatgaattgg tcaaagtaat ggggcggcat
     5161 aagccagaaa atatcgttat tgaaatggca cgtgaaaatc agacaactca aaagggccag
     5221 aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta tcaaagaatt aggaagtcag
     5281 attcttaaag agcatcctgt tgaaaatact caattgcaaa atgaaaagct ctatctctat
     5341 tatctccaaa atggaagaga catgtatgtg gaccaagaat tagatattaa tcgtttaagt
     5401 gattatgatg tcgatgccat tgttccacaa agtttcctta aagacgattc aatagacaat
     5461 aaggtcttaa cgcgttctga taaaaatcgt ggtaaatcgg ataacgttcc aagtgaagaa
     5521 gtagtcaaaa agatgaaaaa ctattggaga caacttctaa acgccaagtt aatcactcaa
     5581 cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt tgagtgaact tgataaagct
     5641 ggttttatca aacgccaatt ggttgaaact cgccaaatca ctaagcatgt ggcacaaatt
     5701 ttggatagtc gcatgaatac taaatacgat gaaaatgata aacttattcg agaggttaaa
     5761 gtgattacct taaaatctaa attagtttct gacttccgaa aagatttcca attctataaa
     5821 gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt atctaaatgc cgtcgttgga
     5881 actgctttga ttaagaaata tccaaaactt gaatcggagt ttgtctatgg tgattataaa
     5941 gtttatgatg ttcgtaaaat gattgctaag tctgagcaag aaataggcaa agcaaccgca
     6001 aaatatttct tttactctaa tatcatgaac ttcttcaaaa cagaaattac acttgcaaat
     6061 ggagagattc gcaaacgccc tctaatcgaa actaatgggg aaactggaga aattgtctgg
     6121 gataaagggc gagattttgc cacagtgcgc aaagtattgt ccatgcccca agtcaatatt
     6181 gtcaagaaaa cagaagtcca gacaggcgga ttctccaagg agtcaatttt accaaaaaga
     6241 aattcggaca agcttattgc tcgtaaaaaa gactgggatc caaaaaaata tggtggtttt
     6301 gatagtccaa cggtagctta ttcagtccta gtggttgcta aggtggaaaa agggaaatcg
     6361 aagaagttaa aatccgttaa agagttacta gggatcacaa ttctggaaag aagttccttt
     6421 gaaaaaaatc cgattgactt tttagaagct aaaggatata aggaagttaa aaaagactta
     6481 atcattaaac tacctaaata tagtcttttt gagttagaaa acggtcgtaa acggatgctg
     6541 gctagtgccg gagaattaca aaaaggaaat gagctggctc tgccaagcaa atatgtgaat
     6601 tttttatatt tagctagtca ttatgaaaag ttgaagggta gtccagaaga taacgaacaa
     6661 aaacaattgt ttgtggagca gcataagcat tatttagatg agattattga gcaaatcagt
     6721 gaattttcta agcgtgttat tttagcagat gccaatttag ataaagttct tagtgcatat
     6781 aacaaacata gagacaaacc aatacgtgaa caagcagaaa atattattca tttatttacg
     6841 ttgacgaatc ttggagctcc cgctgctttt aaatattttg atacaacaat tgatcgtaaa
     6901 cgatatacgt ctacaaaaga agttttagat gccactctta tccatcaatc catcactggt
     6961 ctttatgaaa cacgcattga tttgagtcag ctaggaggtg actaataaaa gcttgatcag
     7021 atcccagctt ttttttgaag tgcctggcag taagttgaaa aaatttgaaa taaacttgga
     7081 ctatgttaat aaaaaaatac aaattaacga acaaaaagat ttgaattatt atataattta
     7141 gttaaaagca attttaaatg agggaggttt ccatcatatg gatagaagtg gtaaacctga
     7201 attaactgct actagtgttg aaaaattttt aattgaaaaa tttgatagtg ttagtgattt
     7261 aatgcaatta agtgaaggtg aagaaagtag agcttttagt tttgatgttg gtggtagagg
     7321 ttatgtttta agagttaata gttgtgctga tggtttttat aaagatagat atgtttatag
     7381 acattttgct agtgctgctt tacctattcc tgaagtttta gatattggtg aatttagtga
     7441 aagtttaact tattgtatta gtagaagagc acaaggtgtt actttacaag atttacctga
     7501 aactgaatta cctgctgttt tacaacctgt tgctgaagct atggatgcta ttgcggcggc
     7561 tgatctcagt caaacttcgg gttttggtcc ttttggtcct caaggtattg gtcaatatac
     7621 tacttggaga gattttattt gtgctattgc tgatcctcat gtttatcatt ggcaaactgt
     7681 tatggatgat actgttagtg ctagtgttgc tcaagcatta gatgaattaa tgttatgggc
     7741 tgaagattgt cctgaagtta gacatttagt tcatgctgat tttggtagta ataatgtttt
     7801 aactgataat ggtagaatta ctgctgttat tgattggagt gaagctatgt ttggtgatag
     7861 tcaatatgaa gttgctaata tttttttttg gagaccttgg ttagcttgta tggaacaaca
     7921 aactagatat tttgaaagaa gacatcctga attagctggt agtcctagat taagagctta
     7981 tatgttaaga attggtttag atcaattata tcaaagttta gttgatggta attttgatga
     8041 tgctgcttgg gctcaaggta gatgtgatgc tattgttaga agtggtgctg gtactgttgg
     8101 tagaactcaa attgctagaa gaagtgctgc tgtttggact gatggttgtg ttgaagtttt
     8161 agctgatagt ggtaatagaa gacctagtac tagacctaga gctaaagaat aatgacgtcg
     8221 ccggagcggt cgagttctgg accgaccggc tcgggttctc atgaccaaaa tcccttaacg
     8281 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8341 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8401 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8461 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8521 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8581 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8641 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8701 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8761 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8821 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8881 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8941 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcactaga
     9001 tccccggcaa gttcatcctt agggttttca tatctcttaa aaagtcaatt tctgcttgat
     9061 taatttcttg tggattattg tttttgcggt ttttcatttt ttatctcctt gagtattgaa
     9121 ttttttctta tatataaata tatagcaaaa actatttttg ccaacttttt tacaaaaaat
     9181 tttttattaa aacacttagg gctttgctaa attctctttt aaaagaactt agtaaagccc
     9241 tatggattta agaactgatt atacttacgt agtaaaaata ctacagatat taatagtata
     9301 gaccatatca aaatcataat caatttatat ttatctaaaa ttgtagtcca tacaaaatac
     9361 aaaaaaatta ggtttttcta aattctattt taaaagaact tagtaacacc taatctctaa
     9421 attacttaca aaattctaaa attagatttt tagttcttca tatttcttta aaagtttttt
     9481 aagaaaatct ttttgatttt caaaaatttc atgcatcata aaactagtaa atttggattt
     9541 ttttttgtaa aaatcataac tttcttgggt tttaagttga aatcttaatg gttttattgg
     9601 attttgccta gattttttta acgccggact ttctttatct tttataaaat tataagaatt
     9661 tttaatacca ttttctatta aataatcttc ttttataagc ccctcttgca gtgcagttgc
     9721 taatcttaaa tacttatacg cttgagtttt tgctacatca aagtctttta taaaatcaag
     9781 aaaagtttta taaccatcaa atttataata ttgattatct tttatttctt ttaaaatttt
     9841 aatattttcc accctgtgga atatttcttt tttgaaattg tcatttaact tttcctttag
     9901 tgtcttgtaa taagaaattt tactttcatt aatcattaaa ttttgttcaa cataagtatc
     9961 tatctttttt gttaaaactt taacaacacg ttttttttcc ataaaaattt tcctttattt
    10021 ttgcaacatt ttccttaatt gatataattg ttactatttt agatttttta atatccactt
    10081 aagaattaat ccctcactag aatatttttt attatagaat tcatatacat gtttttctaa
    10141 atctataaat ttatcaacaa ttgctttatt atagggtgta tctatctttt cttttcttaa
    10201 taatcgtctc atattcttta aataacaaca aacactgccg gttttaaacc taaattctat
    10261 gtaatatatt tttgaaaaag tatatacatt ttcaaccccg tttatttcat actttacaaa
    10321 aacattattt attggttttc tataaccata gtaaatgcct aaaaatttgt ccccgtcttt
    10381 cataggatat agataaagtt tttgaatctc tgatttatct aataattttt caaaaaaaat
    10441 tatacaccgc ttttgtttgt tactaactac tctatgcaat gcctttaata tttttgtgtg
    10501 atatatattt ttatcacctt ttttttcttt cagcaggaag atactttctt ttggaaattt
    10561 aattttttgt tcatttattc ttgcagcctt aagtccgatt ttcatattta atccttatat
    10621 agcctttcta tttaattctt ttaaactcaa taaacttttt tggtttttca ttagttctaa
    10681 taattcgcaa taatatgtgc aaaacacttt attgtattct attttctttt ggttattcaa
    10741 atattctttt ataattggct ttagaagttc aatatttact tcttttttta gttgttcaat
    10801 aaggatattg aaaatgtttg tttttaaatc ttgataattt tgttgagaat tttctttttt
    10861 tctttcaatt gacttctcta atttacgctt tatattgttt aaatcgctat atttatgact
    10921 ttcaataata aaatgaggct taaatttgta attttcgtat attttttgga aacttgtttc
    10981 taattgttta gaattatatt catttttttc aaattctttt tgagtgttgc atagaatttc
    11041 ctttaatttt tcttgttttt ctttcataca agatttagta aaatgtatat ttttattttt
    11101 tagtaattta atttctatta tttttatgat tttaagtatt ttgattatat tatctttatc
    11161 aatatttaaa ttgaataaaa ttgtaagaaa aattgaaaga atttttttac ataaaaagtt
    11221 acatttattg aagtaatttc ttacttgata cttttctatt tcattaatct ttctttcttc
    11281 ttttatatta tttttattat ttaaacactc ctttaaatct acactcccat ttttagtaaa
    11341 tctatcttta aggccccttg aaactctatt ttgaaatctt aaatcttttt tttcttgaaa
    11401 gtatttatag attttttggt aacattcttt ttttgaaaaa ttaagttgat agtatatttc
    11461 ggtccccaaa tttaccccta agtgtttgtg ataattagtt gtgactttaa attctttttc
    11521 taatttgtaa aggtaatttt gcagtgtttt tagtttaacg g
//
