LOCUS       sequence-308846-       10990 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi flaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_P0826S-sgRNAflaB
FEATURES             Location/Qualifiers
     source          1..10990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        733..808
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(2251..2265,2266..2271,2272..2288,2289..2295)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2271..2287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2303..2319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2338..2349
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2356..2382,2383..2385,2386..4872,4873..4875,
                     4876..6462)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6632..7423
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7546..8134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8035..8054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtatatatat cacaattttg atttaaaagt aaattctttt tttaaaaata caaaaatttt
       61 ttataaatta tatagaccat gatattgtat taatactaat tgattttaat ttaaggttga
      121 actaaaataa acatagtttt gtagaaaata atttttcatt attttgctac ttgatattgg
      181 atcgtaaaaa tattagggct ttacaaagtt cttttaaaag agaatttagt aaagccctaa
      241 gtcttttaat aaaaaatttt ttgtaaaaaa gttggcaaaa atagtttttg ctatatattt
      301 atatataaga aaaaattcaa tactcaagga gataaaaaat ggaaaactta aacaataatc
      361 cacaaaaaat taatcaaata aataagcaaa ctaattttat tggttttgaa aattttatag
      421 aacttgataa tcaagttaat caaactaaag aaaaaagtaa attaagtaag ataggtaaga
      481 aattacccgg aataagcagt caagtctagt gcggccggaa acagctatga catgattacg
      541 aattcgagct cggcaataga agaatctata gaaagcataa aaagccttat agaaaacaaa
      601 gaatacatac aaaagttaca ttttataaaa aaattaataa ataaggttta caaaaaatta
      661 aattactttt tttaaaaact aaactttgaa agccttgtta taatataaaa tgaaaattta
      721 aatttctgac ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
      781 acttgaaaaa gtggcaccga gtcggtgctt tttttggcgc gccaaataat gcccgtgtcc
      841 ctgttgaata gggacacggt tttttaacaa tttttttatt cctaggcctt agtggccggt
      901 acccggggat ccttattact ggccgctttc tagcctggat acttgtctgg ctagttgcat
      961 tagagagtca gctagagccc tagggctagc tgtttgtgta ttcggagcca gtgtagtttt
     1021 tctttttact aacgatacgg gtaataattg attgcctttt acagcctgac cttgagacaa
     1081 ttgcaaaagt ctgtccacag aggtttgacc taataatcta aaatcttgtt tgatagtagt
     1141 aagtggaggt atgtagcaac tagaatcttc tgtatcatca taacccacaa ctgaaatatc
     1201 agccccaact ctaagtccac tttcagtaat agctcgcata gctcctaaag ccatttgatc
     1261 attagcaact aacatagcag tagggacaat gccttcattt aacatttgca ttgtttgctg
     1321 aaatcctgac atagcagacc aatctccttc tctttctgct attggttgta tttgatttct
     1381 tgttaaatac ttgtgccatc ctgctaatct caatctggca cttactgaac ttaagggacc
     1441 tgcaagtagt gcaatttgtt gatggcctaa tgcaacaagg tgttcaactc ctaaccttgt
     1501 tccatcttcg tgagaaaaaa ttatagagtt tattggggtt tggtccgaca catccaaaaa
     1561 caatgctgga acattagtac atgctgcttc aactgcaatc gcatcttggt catcaagagg
     1621 ataattaatt ataagtccac taacacgttg agctaataaa ttatgtactg ctgccttaca
     1681 agcctctaca cctgatctct caaccattga tacaacaacg cttgcaccaa gttgatcagc
     1741 tctcgattta attgcggcta caatttgact aggcgcatgt aatgcaagtg atgatgtggc
     1801 aactccaatt aaaagtgatt gtttccccgc aagttgctga gcaacgcgat ttggtatata
     1861 attcaactct gccatagctg cttcaacctt ctctcttgtt tttgcagata catgacttgc
     1921 ttggttcaca actctcgaaa ccgtttggta gctaaccccg gcatattcag caacatcata
     1981 caaagtaaca ggtttcatgg atcattcctc catgataaaa tttaaatttc tgactttggc
     2041 aaatcctttt gcctgttaaa aagaatagca caaaaattaa aaaaaaagaa acgcaaatag
     2101 caatagcatt aaaaataatt attattattt atttctttga ttaaaatcaa aagcctttaa
     2161 tgtattttct tgatctttca tgcgcttaac ggtaaaatcc aaggtattaa gttcaccttc
     2221 cacttttctc tctctatctt ctctagaaaa tcataaaaaa tttatttgct ttgtgagcgg
     2281 ataacaatta taatagattc aattgtgagc ggataacaat ttcacacaga attcattaaa
     2341 gaggagaaat tacatatgga taagaaatac tcaataggct tagctatcgg cacaaatagc
     2401 gtcggatggg cggtgatcac tgatgaatat aaggttccgt ctaaaaagtt caaggttctg
     2461 ggaaatacag accgccacag tatcaaaaaa aatcttatag gggctctttt atttgacagt
     2521 ggagagacag cggaagcgac tcgtctcaaa cggacagctc gtagaaggta tacacgtcgg
     2581 aagaatcgta tttgttatct acaggagatt ttttcaaatg agatggcgaa agtagatgat
     2641 agtttctttc atcgacttga agagtctttt ttggtggaag aagacaagaa gcatgaacgt
     2701 catcctattt ttggaaatat agtagatgaa gttgcttatc atgagaaata tccaactatc
     2761 tatcatctgc gaaaaaaatt ggtagattct actgataaag cggatttgcg cttaatctat
     2821 ttggccttag cgcatatgat taagtttcgt ggtcattttt tgattgaggg agatttaaat
     2881 cctgataata gtgatgtgga caaactattt atccagttgg tacaaaccta caatcaatta
     2941 tttgaagaaa accctattaa cgcaagtgga gtagatgcta aagcgattct ttctgcacga
     3001 ttgagtaaat caagacgatt agaaaatctc attgctcagc tccccggtga gaagaaaaat
     3061 ggcttatttg ggaatctcat tgctttgtca ttgggtttga cccctaattt taaatcaaat
     3121 tttgatttgg cagaagatgc taaattacag ctttcaaaag atacttacga tgatgattta
     3181 gataatttat tggcgcaaat tggagatcaa tatgctgatt tgtttttggc agctaagaat
     3241 ttatcagatg ctattttact ttcagatatc ctaagagtaa atactgaaat aactaaggct
     3301 cccctatcag cttcaatgat taaacgctac gatgaacatc atcaagactt gactctttta
     3361 aaagctttag ttcgacaaca acttccagaa aagtataaag aaatcttttt tgatcaatca
     3421 aaaaacggat atgcaggtta tattgatggg ggagctagcc aagaagaatt ttataaattt
     3481 atcaaaccaa ttttagaaaa aatggatggt actgaggaat tattggtgaa actaaatcgt
     3541 gaagatttgc tgcgcaagca acggaccttt gacaacggct ctattcccca tcaaattcac
     3601 ttgggtgagc tgcatgctat tttgagaaga caagaagact tttatccatt tttaaaagac
     3661 aatcgtgaga agattgaaaa aatcttgact tttcgaattc cttattatgt tggtccattg
     3721 gcgcgtggca atagtcgttt tgcatggatg actcggaagt ctgaagaaac aattacccca
     3781 tggaattttg aagaagttgt cgataaaggt gcttcagctc aatcatttat tgaacgcatg
     3841 acaaactttg ataaaaatct tccaaatgaa aaagtactac caaaacatag tttgctttat
     3901 gagtatttta cggtttataa cgaattgaca aaggtcaaat atgttactga aggaatgcga
     3961 aaaccagcat ttctttcagg tgaacagaag aaagccattg ttgatttact cttcaaaaca
     4021 aatcgaaaag taaccgttaa gcaattaaaa gaagattatt tcaaaaaaat agaatgtttt
     4081 gatagtgttg aaatttcagg agttgaagat agatttaatg cttcattagg tacctaccat
     4141 gatttgctaa aaattattaa agataaagat tttttggata atgaagaaaa tgaagatatc
     4201 ttagaggata ttgttttaac attgacctta tttgaagata gggagatgat tgaggaaaga
     4261 cttaaaacat atgctcacct ctttgatgat aaggtgatga aacagcttaa acgtcgccgt
     4321 tatactggtt ggggacgttt gtctcgaaaa ttgattaatg gtattaggga taagcaatct
     4381 ggcaaaacaa tattagattt tttgaaatca gatggttttg ccaatcgcaa ttttatgcag
     4441 ctgatccatg atgatagttt gacatttaaa gaagacattc aaaaagcaca agtgtctgga
     4501 caaggcgata gtttacatga acatattgca aatttagctg gtagccctgc tattaaaaaa
     4561 ggtattttac agactgtaaa agttgttgat gaattggtca aagtaatggg gcggcataag
     4621 ccagaaaata tcgttattga aatggcacgt gaaaatcaga caactcaaaa gggccagaaa
     4681 aattcgcgag agcgtatgaa acgaatcgaa gaaggtatca aagaattagg aagtcagatt
     4741 cttaaagagc atcctgttga aaatactcaa ttgcaaaatg aaaagctcta tctctattat
     4801 ctccaaaatg gaagagacat gtatgtggac caagaattag atattaatcg tttaagtgat
     4861 tatgatgtcg atgccattgt tccacaaagt ttccttaaag acgattcaat agacaataag
     4921 gtcttaacgc gttctgataa aaatcgtggt aaatcggata acgttccaag tgaagaagta
     4981 gtcaaaaaga tgaaaaacta ttggagacaa cttctaaacg ccaagttaat cactcaacgt
     5041 aagtttgata atttaacgaa agctgaacgt ggaggtttga gtgaacttga taaagctggt
     5101 tttatcaaac gccaattggt tgaaactcgc caaatcacta agcatgtggc acaaattttg
     5161 gatagtcgca tgaatactaa atacgatgaa aatgataaac ttattcgaga ggttaaagtg
     5221 attaccttaa aatctaaatt agtttctgac ttccgaaaag atttccaatt ctataaagta
     5281 cgtgagatta acaattacca tcatgcccat gatgcgtatc taaatgccgt cgttggaact
     5341 gctttgatta agaaatatcc aaaacttgaa tcggagtttg tctatggtga ttataaagtt
     5401 tatgatgttc gtaaaatgat tgctaagtct gagcaagaaa taggcaaagc aaccgcaaaa
     5461 tatttctttt actctaatat catgaacttc ttcaaaacag aaattacact tgcaaatgga
     5521 gagattcgca aacgccctct aatcgaaact aatggggaaa ctggagaaat tgtctgggat
     5581 aaagggcgag attttgccac agtgcgcaaa gtattgtcca tgccccaagt caatattgtc
     5641 aagaaaacag aagtccagac aggcggattc tccaaggagt caattttacc aaaaagaaat
     5701 tcggacaagc ttattgctcg taaaaaagac tgggatccaa aaaaatatgg tggttttgat
     5761 agtccaacgg tagcttattc agtcctagtg gttgctaagg tggaaaaagg gaaatcgaag
     5821 aagttaaaat ccgttaaaga gttactaggg atcacaattc tggaaagaag ttcctttgaa
     5881 aaaaatccga ttgacttttt agaagctaaa ggatataagg aagttaaaaa agacttaatc
     5941 attaaactac ctaaatatag tctttttgag ttagaaaacg gtcgtaaacg gatgctggct
     6001 agtgccggag aattacaaaa aggaaatgag ctggctctgc caagcaaata tgtgaatttt
     6061 ttatatttag ctagtcatta tgaaaagttg aagggtagtc cagaagataa cgaacaaaaa
     6121 caattgtttg tggagcagca taagcattat ttagatgaga ttattgagca aatcagtgaa
     6181 ttttctaagc gtgttatttt agcagatgcc aatttagata aagttcttag tgcatataac
     6241 aaacatagag acaaaccaat acgtgaacaa gcagaaaata ttattcattt atttacgttg
     6301 acgaatcttg gagctcccgc tgcttttaaa tattttgata caacaattga tcgtaaacga
     6361 tatacgtcta caaaagaagt tttagatgcc actcttatcc atcaatccat cactggtctt
     6421 tatgaaacac gcattgattt gagtcagcta ggaggtgact aataaaagct tgatcagatc
     6481 tcagcttttt tttgaagtgc ctggcagtaa gttgaaaaaa tttgaaataa acttggacta
     6541 tgttaataaa aaaatacaaa ttaacgaaca aaaagatttg aattattata taatttagtt
     6601 aaaagcaatt ttaaatgagg gaggtttcca tatgagggaa gcggtgatcg ccgaagtatc
     6661 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     6721 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     6781 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     6841 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     6901 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     6961 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     7021 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     7081 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     7141 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     7201 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     7261 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     7321 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     7381 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgacgtc gccggagcgg
     7441 tcgagttctg gaccgaccgg ctcgggttct catgaccaaa atcccttaac gtgagttttc
     7501 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7561 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7621 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7681 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7741 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7801 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7861 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7921 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7981 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8041 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8101 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8161 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcactag atccccggca
     8221 agttcatcct tagggttttc atatctctta aaaagtcaat ttctgcttga ttaatttctt
     8281 gtggattatt gtttttgcgg tttttcattt tttatctcct tgagtattga attttttctt
     8341 atatataaat atatagcaaa aactattttt gccaactttt ttacaaaaaa ttttttatta
     8401 aaacacttag ggctttgcta aattctcttt taaaagaact tagtaaagcc ctatggattt
     8461 aagaactgat tatacttacg tagtaaaaat actacagata ttaatagtat agaccatatc
     8521 aaaatcataa tcaatttata tttatctaaa attgtagtcc atacaaaata caaaaaaatt
     8581 aggtttttct aaattctatt ttaaaagaac ttagtaacac ctaatctcta aattacttac
     8641 aaaattctaa aattagattt ttagttcttc atatttcttt aaaagttttt taagaaaatc
     8701 tttttgattt tcaaaaattt catgcatcat aaaactagta aatttggatt tttttttgta
     8761 aaaatcataa ctttcttggg ttttaagttg aaatcttaat ggttttattg gattttgcct
     8821 agattttttt aacgccggac tttctttatc ttttataaaa ttataagaat ttttaatacc
     8881 attttctatt aaataatctt cttttataag cccctcttgc agtgcagttg ctaatcttaa
     8941 atacttatac gcttgagttt ttgctacatc aaagtctttt ataaaatcaa gaaaagtttt
     9001 ataaccatca aatttataat attgattatc ttttatttct tttaaaattt taatattttc
     9061 caccctgtgg aatatttctt ttttgaaatt gtcatttaac ttttccttta gtgtcttgta
     9121 ataagaaatt ttactttcat taatcattaa attttgttca acataagtat ctatcttttt
     9181 tgttaaaact ttaacaacac gttttttttc cataaaaatt ttcctttatt tttgcaacat
     9241 tttccttaat tgatataatt gttactattt tagatttttt aatatccact taagaattaa
     9301 tccctcacta gaatattttt tattatagaa ttcatataca tgtttttcta aatctataaa
     9361 tttatcaaca attgctttat tatagggtgt atctatcttt tcttttctta ataatcgtct
     9421 catattcttt aaataacaac aaacactgcc ggttttaaac ctaaattcta tgtaatatat
     9481 ttttgaaaaa gtatatacat tttcaacccc gtttatttca tactttacaa aaacattatt
     9541 tattggtttt ctataaccat agtaaatgcc taaaaatttg tccccgtctt tcataggata
     9601 tagataaagt ttttgaatct ctgatttatc taataatttt tcaaaaaaaa ttatacaccg
     9661 cttttgtttg ttactaacta ctctatgcaa tgcctttaat atttttgtgt gatatatatt
     9721 tttatcacct tttttttctt tcagcaggaa gatactttct tttggaaatt taattttttg
     9781 ttcatttatt cttgcagcct taagtccgat tttcatattt aatccttata tagcctttct
     9841 atttaattct tttaaactca ataaactttt ttggtttttc attagttcta ataattcgca
     9901 ataatatgtg caaaacactt tattgtattc tattttcttt tggttattca aatattcttt
     9961 tataattggc tttagaagtt caatatttac ttcttttttt agttgttcaa taaggatatt
    10021 gaaaatgttt gtttttaaat cttgataatt ttgttgagaa ttttcttttt ttctttcaat
    10081 tgacttctct aatttacgct ttatattgtt taaatcgcta tatttatgac tttcaataat
    10141 aaaatgaggc ttaaatttgt aattttcgta tattttttgg aaacttgttt ctaattgttt
    10201 agaattatat tcattttttt caaattcttt ttgagtgttg catagaattt cctttaattt
    10261 ttcttgtttt tctttcatac aagatttagt aaaatgtata tttttatttt ttagtaattt
    10321 aatttctatt atttttatga ttttaagtat tttgattata ttatctttat caatatttaa
    10381 attgaataaa attgtaagaa aaattgaaag aattttttta cataaaaagt tacatttatt
    10441 gaagtaattt cttacttgat acttttctat ttcattaatc tttctttctt cttttatatt
    10501 atttttatta tttaaacact cctttaaatc tacactccca tttttagtaa atctatcttt
    10561 aaggcccctt gaaactctat tttgaaatct taaatctttt ttttcttgaa agtatttata
    10621 gattttttgg taacattctt tttttgaaaa attaagttga tagtatattt cggtccccaa
    10681 atttacccct aagtgtttgt gataattagt tgtgacttta aattcttttt ctaatttgta
    10741 aaggtaattt tgcagtgttt ttagtttaac gggtgtttga ctatttcttt taaggttttc
    10801 attaaagtaa tagagtatgt ttttttgtgt gtatttctta tatgttttat ttatgtacgc
    10861 tagtgttgag ataaggacta ctaatttata ctgtaattta ttgtagcaag ttgtggtttt
    10921 actggttttg tttatattag ataagttttc catttatagc tccttgaata ccggattttt
    10981 tatctataaa
//
