LOCUS       sequence-308847-       11057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9G_arr2
FEATURES             Location/Qualifiers
     source          1..11057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(3037..3051,3052..3057,3058..3074,3075..3081)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    3057..3073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3089..3105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3124..3135
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(3142..3168,3169..3171,3172..5658,5659..5661,
                     5662..7248)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7418..7951
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      8074..8662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8563..8582
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctacactccc atttttagta aatctatctt taaggcccct tgaaactcta ttttgaaatc
       61 ttaaatcttt tttttcttga aagtatttat agattttttg gtaacattct ttttttgaaa
      121 aattaagttg atagtatatt tcggtcccca aatttacccc taagtgtttg tgataattag
      181 ttgtgacttt aaattctttt tctaatttgt aaaggtaatt ttgcagtgtt tttagtttaa
      241 cgggtgtttg actatttctt ttaaggtttt cattaaagta atagagtatg tttttttgtg
      301 tgtatttctt atatgtttta tttatgtacg ctagtgttga gataaggact actaatttat
      361 actgtaattt attgtagcaa gttgtggttt tactggtttt gtttatatta gataagtttt
      421 ccatttatag ctccttgaat accggatttt ttatctataa agtatatata tcacaatttt
      481 gatttaaaag taaattcttt ttttaaaaat acaaaaattt tttataaatt atatagacca
      541 tgatattgta ttaatactaa ttgattttaa tttaaggttg aactaaaata aacatagttt
      601 tgtagaaaat aatttttcat tattttgcta cttgatattg gatcgtaaaa atattagggc
      661 tttacaaagt tcttttaaaa gagaatttag taaagcccta agtcttttaa taaaaaattt
      721 tttgtaaaaa agttggcaaa aatagttttt gctatatatt tatatataag aaaaaattca
      781 atactcaagg agataaaaaa tggaaaactt aaacaataat ccacaaaaaa ttaatcaaat
      841 aaataagcaa actaatttta ttggttttga aaattttata gaacttgata atcaagttaa
      901 tcaaactaaa gaaaaaagta aattaagtaa gataggtaag aaattacccg gaataagcag
      961 tcaagtctag tgcggccgca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     1021 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     1081 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgaggaggta
     1141 acgtcatggt aaaagattgg attcctattt ctcatgataa ttataaacaa gtacaaggac
     1201 cattttatca tggaactaaa gctaatttag caattggtga tttactaact acaggattta
     1261 tttctcattt tgaagatggt cgaattctta aacatattta tttttcagct ttaatggaac
     1321 cagcagtttg gggagctgaa cttgctatgt cactatctgg tcttgaaggt cgtggttata
     1381 tatatatagt tgaaccaaca ggaccatttg aagatgatcc aaatcttaca aataaaaaat
     1441 ttcctggtaa tccaacacaa tcttatagaa cttgtgaacc tttaagaatt gttggtgttg
     1501 ttgaagattg ggaaggacat cctgttgaat taataagagg aatgttagat tcattagaag
     1561 atttaaaacg tcgtggttta catgttattg aagattaacc tggcgcgcca aataatgccc
     1621 gtgtccctgt tgaataggga cacggttttt taacaatttt tttattccta ggccttagtg
     1681 gccggtaccc ggggatcctt attactggcc gctttctagc ctggatactt gtctggctag
     1741 ttgcattaga gagtcagcta gagccctagg gctagctgtt tgtgtattcg gagccagtgt
     1801 agtttttctt tttactaacg atacgggtaa taattgattg ccttttacag cctgaccttg
     1861 agacaattgc aaaagtctgt ccacagaggt ttgacctaat aatctaaaat cttgtttgat
     1921 agtagtaagt ggaggtatgt agcaactaga atcttctgta tcatcataac ccacaactga
     1981 aatatcagcc ccaactctaa gtccactttc agtaatagct cgcatagctc ctaaagccat
     2041 ttgatcatta gcaactaaca tagcagtagg gacaatgcct tcatttaaca tttgcattgt
     2101 ttgctgaaat cctgacatag cagaccaatc tccttctctt tctgctattg gttgtatttg
     2161 atttcttgtt aaatacttgt gccatcctgc taatctcaat ctggcactta ctgaacttaa
     2221 gggacctgca agtagtgcaa tttgttgatg gcctaatgca acaaggtgtt caactcctaa
     2281 ccttgttcca tcttcgtgag aaaaaattat agagtttatt ggggtttggt ccgacacatc
     2341 caaaaacaat gctggaacat tagtacatgc tgcttcaact gcaatcgcat cttggtcatc
     2401 aagaggataa ttaattataa gtccactaac acgttgagct aataaattat gtactgctgc
     2461 cttacaagcc tctacacctg atctctcaac cattgataca acaacgcttg caccaagttg
     2521 atcagctctc gatttaattg cggctacaat ttgactaggc gcatgtaatg caagtgatga
     2581 tgtggcaact ccaattaaaa gtgattgttt ccccgcaagt tgctgagcaa cgcgatttgg
     2641 tatataattc aactctgcca tagctgcttc aaccttctct cttgtttttg cagatacatg
     2701 acttgcttgg ttcacaactc tcgaaaccgt ttggtagcta accccggcat attcagcaac
     2761 atcatacaaa gtaacaggtt tcatggatca ttcctccatg ataaaattta aatttctgac
     2821 tttggcaaat ccttttgcct gttaaaaaga atagcacaaa aattaaaaaa aaagaaacgc
     2881 aaatagcaat agcattaaaa ataattatta ttatttattt ctttgattaa aatcaaaagc
     2941 ctttaatgta ttttcttgat ctttcatgcg cttaacggta aaatccaagg tattaagttc
     3001 accttccact tttctctctc tatcttctct agaaaatcat aaaaaattta tttgctttgt
     3061 gagcggataa caattataat agattcaatt gtgagcggat aacaatttca cacagaattc
     3121 attaaagagg agaaattaca tatggataag aaatactcaa taggcttagc tatcggcaca
     3181 aatagcgtcg gatgggcggt gatcactgat gaatataagg ttccgtctaa aaagttcaag
     3241 gttctgggaa atacagaccg ccacagtatc aaaaaaaatc ttataggggc tcttttattt
     3301 gacagtggag agacagcgga agcgactcgt ctcaaacgga cagctcgtag aaggtataca
     3361 cgtcggaaga atcgtatttg ttatctacag gagatttttt caaatgagat ggcgaaagta
     3421 gatgatagtt tctttcatcg acttgaagag tcttttttgg tggaagaaga caagaagcat
     3481 gaacgtcatc ctatttttgg aaatatagta gatgaagttg cttatcatga gaaatatcca
     3541 actatctatc atctgcgaaa aaaattggta gattctactg ataaagcgga tttgcgctta
     3601 atctatttgg ccttagcgca tatgattaag tttcgtggtc attttttgat tgagggagat
     3661 ttaaatcctg ataatagtga tgtggacaaa ctatttatcc agttggtaca aacctacaat
     3721 caattatttg aagaaaaccc tattaacgca agtggagtag atgctaaagc gattctttct
     3781 gcacgattga gtaaatcaag acgattagaa aatctcattg ctcagctccc cggtgagaag
     3841 aaaaatggct tatttgggaa tctcattgct ttgtcattgg gtttgacccc taattttaaa
     3901 tcaaattttg atttggcaga agatgctaaa ttacagcttt caaaagatac ttacgatgat
     3961 gatttagata atttattggc gcaaattgga gatcaatatg ctgatttgtt tttggcagct
     4021 aagaatttat cagatgctat tttactttca gatatcctaa gagtaaatac tgaaataact
     4081 aaggctcccc tatcagcttc aatgattaaa cgctacgatg aacatcatca agacttgact
     4141 cttttaaaag ctttagttcg acaacaactt ccagaaaagt ataaagaaat cttttttgat
     4201 caatcaaaaa acggatatgc aggttatatt gatgggggag ctagccaaga agaattttat
     4261 aaatttatca aaccaatttt agaaaaaatg gatggtactg aggaattatt ggtgaaacta
     4321 aatcgtgaag atttgctgcg caagcaacgg acctttgaca acggctctat tccccatcaa
     4381 attcacttgg gtgagctgca tgctattttg agaagacaag aagactttta tccattttta
     4441 aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc gaattcctta ttatgttggt
     4501 ccattggcgc gtggcaatag tcgttttgca tggatgactc ggaagtctga agaaacaatt
     4561 accccatgga attttgaaga agttgtcgat aaaggtgctt cagctcaatc atttattgaa
     4621 cgcatgacaa actttgataa aaatcttcca aatgaaaaag tactaccaaa acatagtttg
     4681 ctttatgagt attttacggt ttataacgaa ttgacaaagg tcaaatatgt tactgaagga
     4741 atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag ccattgttga tttactcttc
     4801 aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa
     4861 tgttttgata gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc attaggtacc
     4921 taccatgatt tgctaaaaat tattaaagat aaagattttt tggataatga agaaaatgaa
     4981 gatatcttag aggatattgt tttaacattg accttatttg aagataggga gatgattgag
     5041 gaaagactta aaacatatgc tcacctcttt gatgataagg tgatgaaaca gcttaaacgt
     5101 cgccgttata ctggttgggg acgtttgtct cgaaaattga ttaatggtat tagggataag
     5161 caatctggca aaacaatatt agattttttg aaatcagatg gttttgccaa tcgcaatttt
     5221 atgcagctga tccatgatga tagtttgaca tttaaagaag acattcaaaa agcacaagtg
     5281 tctggacaag gcgatagttt acatgaacat attgcaaatt tagctggtag ccctgctatt
     5341 aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg
     5401 cataagccag aaaatatcgt tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc
     5461 cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag gtatcaaaga attaggaagt
     5521 cagattctta aagagcatcc tgttgaaaat actcaattgc aaaatgaaaa gctctatctc
     5581 tattatctcc aaaatggaag agacatgtat gtggaccaag aattagatat taatcgttta
     5641 agtgattatg atgtcgatgc cattgttcca caaagtttcc ttaaagacga ttcaatagac
     5701 aataaggtct taacgcgttc tgataaaaat cgtggtaaat cggataacgt tccaagtgaa
     5761 gaagtagtca aaaagatgaa aaactattgg agacaacttc taaacgccaa gttaatcact
     5821 caacgtaagt ttgataattt aacgaaagct gaacgtggag gtttgagtga acttgataaa
     5881 gctggtttta tcaaacgcca attggttgaa actcgccaaa tcactaagca tgtggcacaa
     5941 attttggata gtcgcatgaa tactaaatac gatgaaaatg ataaacttat tcgagaggtt
     6001 aaagtgatta ccttaaaatc taaattagtt tctgacttcc gaaaagattt ccaattctat
     6061 aaagtacgtg agattaacaa ttaccatcat gcccatgatg cgtatctaaa tgccgtcgtt
     6121 ggaactgctt tgattaagaa atatccaaaa cttgaatcgg agtttgtcta tggtgattat
     6181 aaagtttatg atgttcgtaa aatgattgct aagtctgagc aagaaatagg caaagcaacc
     6241 gcaaaatatt tcttttactc taatatcatg aacttcttca aaacagaaat tacacttgca
     6301 aatggagaga ttcgcaaacg ccctctaatc gaaactaatg gggaaactgg agaaattgtc
     6361 tgggataaag ggcgagattt tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat
     6421 attgtcaaga aaacagaagt ccagacaggc ggattctcca aggagtcaat tttaccaaaa
     6481 agaaattcgg acaagcttat tgctcgtaaa aaagactggg atccaaaaaa atatggtggt
     6541 tttgatagtc caacggtagc ttattcagtc ctagtggttg ctaaggtgga aaaagggaaa
     6601 tcgaagaagt taaaatccgt taaagagtta ctagggatca caattctgga aagaagttcc
     6661 tttgaaaaaa atccgattga ctttttagaa gctaaaggat ataaggaagt taaaaaagac
     6721 ttaatcatta aactacctaa atatagtctt tttgagttag aaaacggtcg taaacggatg
     6781 ctggctagtg ccggagaatt acaaaaagga aatgagctgg ctctgccaag caaatatgtg
     6841 aattttttat atttagctag tcattatgaa aagttgaagg gtagtccaga agataacgaa
     6901 caaaaacaat tgtttgtgga gcagcataag cattatttag atgagattat tgagcaaatc
     6961 agtgaatttt ctaagcgtgt tattttagca gatgccaatt tagataaagt tcttagtgca
     7021 tataacaaac atagagacaa accaatacgt gaacaagcag aaaatattat tcatttattt
     7081 acgttgacga atcttggagc tcccgctgct tttaaatatt ttgatacaac aattgatcgt
     7141 aaacgatata cgtctacaaa agaagtttta gatgccactc ttatccatca atccatcact
     7201 ggtctttatg aaacacgcat tgatttgagt cagctaggag gtgactaata aaagcttgat
     7261 cagatcccag cttttttttg aagtgcctgg cagtaagttg aaaaaatttg aaataaactt
     7321 ggactatgtt aataaaaaaa tacaaattaa cgaacaaaaa gatttgaatt attatataat
     7381 ttagttaaaa gcaattttaa atgagggagg tttccatatg ttacgcagca gcaacgatgt
     7441 tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg
     7501 cacatgtagg ctcggccctg accaagtcaa atccatgcgg gctgctcttg atcttttcgg
     7561 tcgtgagttc ggagacgtag ccacctactc ccaacatcag ccggactccg attacctcgg
     7621 gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt
     7681 tggcgctctc gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg agatctatat
     7741 ctatgatctc gcagtctccg gcgagcaccg gaggcagggc attgccaccg cgctcatcaa
     7801 tctcctcaag catgaggcca acgcgcttgg tgcttatgtg atctacgtgc aagcagatta
     7861 cggtgacgat cccgcagtgg ctctctatac aaagttgggc atacgggaag aagtgatgca
     7921 ctttgatatc gacccaagta ccgccaccta atgacgtcgc cggagcggtc gagttctgga
     7981 ccgaccggct cgggttctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8041 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8101 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8161 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8221 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8281 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8341 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8401 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8461 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8521 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8581 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8641 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8701 ttgctggcct tttgctcaca tgttctttcc tgcactagat ccccggcaag ttcatcctta
     8761 gggttttcat atctcttaaa aagtcaattt ctgcttgatt aatttcttgt ggattattgt
     8821 ttttgcggtt tttcattttt tatctccttg agtattgaat tttttcttat atataaatat
     8881 atagcaaaaa ctatttttgc caactttttt acaaaaaatt ttttattaaa acacttaggg
     8941 ctttgctaaa ttctctttta aaagaactta gtaaagccct atggatttaa gaactgatta
     9001 tacttacgta gtaaaaatac tacagatatt aatagtatag accatatcaa aatcataatc
     9061 aatttatatt tatctaaaat tgtagtccat acaaaataca aaaaaattag gtttttctaa
     9121 attctatttt aaaagaactt agtaacacct aatctctaaa ttacttacaa aattctaaaa
     9181 ttagattttt agttcttcat atttctttaa aagtttttta agaaaatctt tttgattttc
     9241 aaaaatttca tgcatcataa aactagtaaa tttggatttt tttttgtaaa aatcataact
     9301 ttcttgggtt ttaagttgaa atcttaatgg ttttattgga ttttgcctag atttttttaa
     9361 cgccggactt tctttatctt ttataaaatt ataagaattt ttaataccat tttctattaa
     9421 ataatcttct tttataagcc cctcttgcag tgcagttgct aatcttaaat acttatacgc
     9481 ttgagttttt gctacatcaa agtcttttat aaaatcaaga aaagttttat aaccatcaaa
     9541 tttataatat tgattatctt ttatttcttt taaaatttta atattttcca ccctgtggaa
     9601 tatttctttt ttgaaattgt catttaactt ttcctttagt gtcttgtaat aagaaatttt
     9661 actttcatta atcattaaat tttgttcaac ataagtatct atcttttttg ttaaaacttt
     9721 aacaacacgt tttttttcca taaaaatttt cctttatttt tgcaacattt tccttaattg
     9781 atataattgt tactatttta gattttttaa tatccactta agaattaatc cctcactaga
     9841 atatttttta ttatagaatt catatacatg tttttctaaa tctataaatt tatcaacaat
     9901 tgctttatta tagggtgtat ctatcttttc ttttcttaat aatcgtctca tattctttaa
     9961 ataacaacaa acactgccgg ttttaaacct aaattctatg taatatattt ttgaaaaagt
    10021 atatacattt tcaaccccgt ttatttcata ctttacaaaa acattattta ttggttttct
    10081 ataaccatag taaatgccta aaaatttgtc cccgtctttc ataggatata gataaagttt
    10141 ttgaatctct gatttatcta ataatttttc aaaaaaaatt atacaccgct tttgtttgtt
    10201 actaactact ctatgcaatg cctttaatat ttttgtgtga tatatatttt tatcaccttt
    10261 tttttctttc agcaggaaga tactttcttt tggaaattta attttttgtt catttattct
    10321 tgcagcctta agtccgattt tcatatttaa tccttatata gcctttctat ttaattcttt
    10381 taaactcaat aaactttttt ggtttttcat tagttctaat aattcgcaat aatatgtgca
    10441 aaacacttta ttgtattcta ttttcttttg gttattcaaa tattctttta taattggctt
    10501 tagaagttca atatttactt ctttttttag ttgttcaata aggatattga aaatgtttgt
    10561 ttttaaatct tgataatttt gttgagaatt ttcttttttt ctttcaattg acttctctaa
    10621 tttacgcttt atattgttta aatcgctata tttatgactt tcaataataa aatgaggctt
    10681 aaatttgtaa ttttcgtata ttttttggaa acttgtttct aattgtttag aattatattc
    10741 atttttttca aattcttttt gagtgttgca tagaatttcc tttaattttt cttgtttttc
    10801 tttcatacaa gatttagtaa aatgtatatt tttatttttt agtaatttaa tttctattat
    10861 ttttatgatt ttaagtattt tgattatatt atctttatca atatttaaat tgaataaaat
    10921 tgtaagaaaa attgaaagaa tttttttaca taaaaagtta catttattga agtaatttct
    10981 tacttgatac ttttctattt cattaatctt tctttcttct tttatattat ttttattatt
    11041 taaacactcc tttaaat
//
