LOCUS       sequence-308848-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi flaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(-10TC)_Psyn-sgRNAflaB
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..35
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        57..132
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(1575..1589,1590..1595,1596..1612,1613..1619)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1595..1611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1627..1643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1662..1673
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1680..1706,1707..1709,1710..4196,4197..4199,
                     4200..5786)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5956..6747
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6870..7458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7359..7378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgacagcta gctcagtcct aggtataata ctagtgaaaa tttaaatttc tgacttgttt
       61 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
      121 ccgagtcggt gctttttttg gcgcgccaaa taatgcccgt gtccctgttg aatagggaca
      181 cggtttttta acaatttttt tattcctagg ccttagtggc cggtacccgg ggatccttat
      241 tactggccgc tttctagcct ggatacttgt ctggctagtt gcattagaga gtcagctaga
      301 gccctagggc tagctgtttg tgtattcgga gccagtgtag tttttctttt tactaacgat
      361 acgggtaata attgattgcc ttttacagcc tgaccttgag acaattgcaa aagtctgtcc
      421 acagaggttt gacctaataa tctaaaatct tgtttgatag tagtaagtgg aggtatgtag
      481 caactagaat cttctgtatc atcataaccc acaactgaaa tatcagcccc aactctaagt
      541 ccactttcag taatagctcg catagctcct aaagccattt gatcattagc aactaacata
      601 gcagtaggga caatgccttc atttaacatt tgcattgttt gctgaaatcc tgacatagca
      661 gaccaatctc cttctctttc tgctattggt tgtatttgat ttcttgttaa atacttgtgc
      721 catcctgcta atctcaatct ggcacttact gaacttaagg gacctgcaag tagtgcaatt
      781 tgttgatggc ctaatgcaac aaggtgttca actcctaacc ttgttccatc ttcgtgagaa
      841 aaaattatag agtttattgg ggtttggtcc gacacatcca aaaacaatgc tggaacatta
      901 gtacatgctg cttcaactgc aatcgcatct tggtcatcaa gaggataatt aattataagt
      961 ccactaacac gttgagctaa taaattatgt actgctgcct tacaagcctc tacacctgat
     1021 ctctcaacca ttgatacaac aacgcttgca ccaagttgat cagctctcga tttaattgcg
     1081 gctacaattt gactaggcgc atgtaatgca agtgatgatg tggcaactcc aattaaaagt
     1141 gattgtttcc ccgcaagttg ctgagcaacg cgatttggta tataattcaa ctctgccata
     1201 gctgcttcaa ccttctctct tgtttttgca gatacatgac ttgcttggtt cacaactctc
     1261 gaaaccgttt ggtagctaac cccggcatat tcagcaacat catacaaagt aacaggtttc
     1321 atggatcatt cctccatgat aaaatttaaa tttctgactt tggcaaatcc ttttgcctgt
     1381 taaaaagaat agcacaaaaa ttaaaaaaaa agaaacgcaa atagcaatag cattaaaaat
     1441 aattattatt atttatttct ttgattaaaa tcaaaagcct ttaatgtatt ttcttgatct
     1501 ttcatgcgct taacggtaaa atccaaggta ttaagttcac cttccacttt tctctctcta
     1561 tcttctctag aaaatcataa aaaatttatt tgctttgtga gcggataaca attataacag
     1621 attcaattgt gagcggataa caatttcaca cagaattcat taaagaggag aaattacata
     1681 tggataagaa atactcaata ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga
     1741 tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc
     1801 acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag
     1861 cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt
     1921 atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac
     1981 ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa
     2041 atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa
     2101 aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata
     2161 tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg
     2221 tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta
     2281 ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac
     2341 gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc
     2401 tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag
     2461 atgctaaatt acagctttca aaagatactt acgatgatga tttagataat ttattggcgc
     2521 aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt
     2581 tactttcaga tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa
     2641 tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac
     2701 aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag
     2761 gttatattga tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag
     2821 aaaaaatgga tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca
     2881 agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg
     2941 ctattttgag aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg
     3001 aaaaaatctt gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc
     3061 gttttgcatg gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag
     3121 ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa
     3181 atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt
     3241 ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt
     3301 caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg
     3361 ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt
     3421 caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta
     3481 ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt
     3541 taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc
     3601 acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac
     3661 gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag
     3721 attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata
     3781 gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac
     3841 atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg
     3901 taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta
     3961 ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta
     4021 tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg
     4081 ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag
     4141 acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatgcca
     4201 ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg
     4261 ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa
     4321 actattggag acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa
     4381 cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat
     4441 tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata
     4501 ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta
     4561 aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt
     4621 accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat
     4681 atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa
     4741 tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta
     4801 atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc
     4861 ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg
     4921 ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtcc
     4981 agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg
     5041 ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt
     5101 attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta
     5161 aagagttact agggatcaca attctggaaa gaagttcctt tgaaaaaaat ccgattgact
     5221 ttttagaagc taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat
     5281 atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac
     5341 aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc
     5401 attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc
     5461 agcataagca ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta
     5521 ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac
     5581 caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc
     5641 ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag
     5701 aagttttaga tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg
     5761 atttgagtca gctaggaggt gactaataaa agcttgatca gatctcagct tttttttgaa
     5821 gtgcctggca gtaagttgaa aaaatttgaa ataaacttgg actatgttaa taaaaaaata
     5881 caaattaacg aacaaaaaga tttgaattat tatataattt agttaaaagc aattttaaat
     5941 gagggaggtt tccatatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     6001 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     6061 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     6121 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     6181 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     6241 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     6301 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     6361 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     6421 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     6481 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     6541 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     6601 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     6661 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     6721 gagatcacca aggtagtcgg caaataatga cgtcgccgga gcggtcgagt tctggaccga
     6781 ccggctcggg ttctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6841 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6901 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6961 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7021 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7081 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7141 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7201 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7261 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7321 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7381 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7441 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7501 tggccttttg ctcacatgtt ctttcctgca ctagatcccc ggcaagttca tccttagggt
     7561 tttcatatct cttaaaaagt caatttctgc ttgattaatt tcttgtggat tattgttttt
     7621 gcggtttttc attttttatc tccttgagta ttgaattttt tcttatatat aaatatatag
     7681 caaaaactat ttttgccaac ttttttacaa aaaatttttt attaaaacac ttagggcttt
     7741 gctaaattct cttttaaaag aacttagtaa agccctatgg atttaagaac tgattatact
     7801 tacgtagtaa aaatactaca gatattaata gtatagacca tatcaaaatc ataatcaatt
     7861 tatatttatc taaaattgta gtccatacaa aatacaaaaa aattaggttt ttctaaattc
     7921 tattttaaaa gaacttagta acacctaatc tctaaattac ttacaaaatt ctaaaattag
     7981 atttttagtt cttcatattt ctttaaaagt tttttaagaa aatctttttg attttcaaaa
     8041 atttcatgca tcataaaact agtaaatttg gatttttttt tgtaaaaatc ataactttct
     8101 tgggttttaa gttgaaatct taatggtttt attggatttt gcctagattt ttttaacgcc
     8161 ggactttctt tatcttttat aaaattataa gaatttttaa taccattttc tattaaataa
     8221 tcttctttta taagcccctc ttgcagtgca gttgctaatc ttaaatactt atacgcttga
     8281 gtttttgcta catcaaagtc ttttataaaa tcaagaaaag ttttataacc atcaaattta
     8341 taatattgat tatcttttat ttcttttaaa attttaatat tttccaccct gtggaatatt
     8401 tcttttttga aattgtcatt taacttttcc tttagtgtct tgtaataaga aattttactt
     8461 tcattaatca ttaaattttg ttcaacataa gtatctatct tttttgttaa aactttaaca
     8521 acacgttttt tttccataaa aattttcctt tatttttgca acattttcct taattgatat
     8581 aattgttact attttagatt ttttaatatc cacttaagaa ttaatccctc actagaatat
     8641 tttttattat agaattcata tacatgtttt tctaaatcta taaatttatc aacaattgct
     8701 ttattatagg gtgtatctat cttttctttt cttaataatc gtctcatatt ctttaaataa
     8761 caacaaacac tgccggtttt aaacctaaat tctatgtaat atatttttga aaaagtatat
     8821 acattttcaa ccccgtttat ttcatacttt acaaaaacat tatttattgg ttttctataa
     8881 ccatagtaaa tgcctaaaaa tttgtccccg tctttcatag gatatagata aagtttttga
     8941 atctctgatt tatctaataa tttttcaaaa aaaattatac accgcttttg tttgttacta
     9001 actactctat gcaatgcctt taatattttt gtgtgatata tatttttatc accttttttt
     9061 tctttcagca ggaagatact ttcttttgga aatttaattt tttgttcatt tattcttgca
     9121 gccttaagtc cgattttcat atttaatcct tatatagcct ttctatttaa ttcttttaaa
     9181 ctcaataaac ttttttggtt tttcattagt tctaataatt cgcaataata tgtgcaaaac
     9241 actttattgt attctatttt cttttggtta ttcaaatatt cttttataat tggctttaga
     9301 agttcaatat ttacttcttt ttttagttgt tcaataagga tattgaaaat gtttgttttt
     9361 aaatcttgat aattttgttg agaattttct ttttttcttt caattgactt ctctaattta
     9421 cgctttatat tgtttaaatc gctatattta tgactttcaa taataaaatg aggcttaaat
     9481 ttgtaatttt cgtatatttt ttggaaactt gtttctaatt gtttagaatt atattcattt
     9541 ttttcaaatt ctttttgagt gttgcataga atttccttta atttttcttg tttttctttc
     9601 atacaagatt tagtaaaatg tatattttta ttttttagta atttaatttc tattattttt
     9661 atgattttaa gtattttgat tatattatct ttatcaatat ttaaattgaa taaaattgta
     9721 agaaaaattg aaagaatttt tttacataaa aagttacatt tattgaagta atttcttact
     9781 tgatactttt ctatttcatt aatctttctt tcttctttta tattattttt attatttaaa
     9841 cactccttta aatctacact cccattttta gtaaatctat ctttaaggcc ccttgaaact
     9901 ctattttgaa atcttaaatc ttttttttct tgaaagtatt tatagatttt ttggtaacat
     9961 tctttttttg aaaaattaag ttgatagtat atttcggtcc ccaaatttac ccctaagtgt
    10021 ttgtgataat tagttgtgac tttaaattct ttttctaatt tgtaaaggta attttgcagt
    10081 gtttttagtt taacgggtgt ttgactattt cttttaaggt tttcattaaa gtaatagagt
    10141 atgttttttt gtgtgtattt cttatatgtt ttatttatgt acgctagtgt tgagataagg
    10201 actactaatt tatactgtaa tttattgtag caagttgtgg ttttactggt tttgtttata
    10261 ttagataagt tttccattta tagctccttg aataccggat tttttatcta taaagtatat
    10321 atatcacaat tttgatttaa aagtaaattc tttttttaaa aatacaaaaa ttttttataa
    10381 attatataga ccatgatatt gtattaatac taattgattt taatttaagg ttgaactaaa
    10441 ataaacatag ttttgtagaa aataattttt cattattttg ctacttgata ttggatcgta
    10501 aaaatattag ggctttacaa agttctttta aaagagaatt tagtaaagcc ctaagtcttt
    10561 taataaaaaa ttttttgtaa aaaagttggc aaaaatagtt tttgctatat atttatatat
    10621 aagaaaaaat tcaatactca aggagataaa aaatggaaaa cttaaacaat aatccacaaa
    10681 aaattaatca aataaataag caaactaatt ttattggttt tgaaaatttt atagaacttg
    10741 ataatcaagt taatcaaact aaagaaaaaa gtaaattaag taagataggt aagaaattac
    10801 ccggaataag cagtcaagtc tagtgcggcc ggaattctaa agatct
//
