LOCUS       sequence-308849-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi mreB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(RBSmut)_Psyn-sgRNAmreB
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1140..1174
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        1196..1271
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(2714..2728,2729..2734,2735..2751,2752..2758)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2734..2750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2766..2782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(2819..2845,2846..2848,2849..5335,5336..5338,
                     5339..6925)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7095..7886
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8009..8597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8498..8517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaataaaatt gtaagaaaaa ttgaaagaat ttttttacat aaaaagttac atttattgaa
       61 gtaatttctt acttgatact tttctatttc attaatcttt ctttcttctt ttatattatt
      121 tttattattt aaacactcct ttaaatctac actcccattt ttagtaaatc tatctttaag
      181 gccccttgaa actctatttt gaaatcttaa atcttttttt tcttgaaagt atttatagat
      241 tttttggtaa cattcttttt ttgaaaaatt aagttgatag tatatttcgg tccccaaatt
      301 tacccctaag tgtttgtgat aattagttgt gactttaaat tctttttcta atttgtaaag
      361 gtaattttgc agtgttttta gtttaacggg tgtttgacta tttcttttaa ggttttcatt
      421 aaagtaatag agtatgtttt tttgtgtgta tttcttatat gttttattta tgtacgctag
      481 tgttgagata aggactacta atttatactg taatttattg tagcaagttg tggttttact
      541 ggttttgttt atattagata agttttccat ttatagctcc ttgaataccg gattttttat
      601 ctataaagta tatatatcac aattttgatt taaaagtaaa ttcttttttt aaaaatacaa
      661 aaatttttta taaattatat agaccatgat attgtattaa tactaattga ttttaattta
      721 aggttgaact aaaataaaca tagttttgta gaaaataatt tttcattatt ttgctacttg
      781 atattggatc gtaaaaatat tagggcttta caaagttctt ttaaaagaga atttagtaaa
      841 gccctaagtc ttttaataaa aaattttttg taaaaaagtt ggcaaaaata gtttttgcta
      901 tatatttata tataagaaaa aattcaatac tcaaggagat aaaaaatgga aaacttaaac
      961 aataatccac aaaaaattaa tcaaataaat aagcaaacta attttattgg ttttgaaaat
     1021 tttatagaac ttgataatca agttaatcaa actaaagaaa aaagtaaatt aagtaagata
     1081 ggtaagaaat tacccggaat aagcagtcaa gtctagtgcg gccggaattc taaagatctt
     1141 tgacagctag ctcagtccta ggtataatac tagtgatatc tattgcaaca acagagtttt
     1201 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     1261 cgagtcggtg ctttttttgg cgcgccaaat aatgcccgtg tccctgttga atagggacac
     1321 ggttttttaa caattttttt attcctaggc cttagtggcc ggtacccggg gatccttatt
     1381 actggccgct ttctagcctg gatacttgtc tggctagttg cattagagag tcagctagag
     1441 ccctagggct agctgtttgt gtattcggag ccagtgtagt ttttcttttt actaacgata
     1501 cgggtaataa ttgattgcct tttacagcct gaccttgaga caattgcaaa agtctgtcca
     1561 cagaggtttg acctaataat ctaaaatctt gtttgatagt agtaagtgga ggtatgtagc
     1621 aactagaatc ttctgtatca tcataaccca caactgaaat atcagcccca actctaagtc
     1681 cactttcagt aatagctcgc atagctccta aagccatttg atcattagca actaacatag
     1741 cagtagggac aatgccttca tttaacattt gcattgtttg ctgaaatcct gacatagcag
     1801 accaatctcc ttctctttct gctattggtt gtatttgatt tcttgttaaa tacttgtgcc
     1861 atcctgctaa tctcaatctg gcacttactg aacttaaggg acctgcaagt agtgcaattt
     1921 gttgatggcc taatgcaaca aggtgttcaa ctcctaacct tgttccatct tcgtgagaaa
     1981 aaattataga gtttattggg gtttggtccg acacatccaa aaacaatgct ggaacattag
     2041 tacatgctgc ttcaactgca atcgcatctt ggtcatcaag aggataatta attataagtc
     2101 cactaacacg ttgagctaat aaattatgta ctgctgcctt acaagcctct acacctgatc
     2161 tctcaaccat tgatacaaca acgcttgcac caagttgatc agctctcgat ttaattgcgg
     2221 ctacaatttg actaggcgca tgtaatgcaa gtgatgatgt ggcaactcca attaaaagtg
     2281 attgtttccc cgcaagttgc tgagcaacgc gatttggtat ataattcaac tctgccatag
     2341 ctgcttcaac cttctctctt gtttttgcag atacatgact tgcttggttc acaactctcg
     2401 aaaccgtttg gtagctaacc ccggcatatt cagcaacatc atacaaagta acaggtttca
     2461 tggatcattc ctccatgata aaatttaaat ttctgacttt ggcaaatcct tttgcctgtt
     2521 aaaaagaata gcacaaaaat taaaaaaaaa gaaacgcaaa tagcaatagc attaaaaata
     2581 attattatta tttatttctt tgattaaaat caaaagcctt taatgtattt tcttgatctt
     2641 tcatgcgctt aacggtaaaa tccaaggtat taagttcacc ttccactttt ctctctctat
     2701 cttctctaga aaatcataaa aaatttattt gctttgtgag cggataacaa ttataataga
     2761 ttcaattgtg agcggataac aatttcacac agaattcatt aaagaagaga aattacatat
     2821 ggataagaaa tactcaatag gcttagctat cggcacaaat agcgtcggat gggcggtgat
     2881 cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca
     2941 cagtatcaaa aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc
     3001 gactcgtctc aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta
     3061 tctacaggag attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact
     3121 tgaagagtct tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa
     3181 tatagtagat gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa
     3241 attggtagat tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat
     3301 gattaagttt cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt
     3361 ggacaaacta tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat
     3421 taacgcaagt ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg
     3481 attagaaaat ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct
     3541 cattgctttg tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga
     3601 tgctaaatta cagctttcaa aagatactta cgatgatgat ttagataatt tattggcgca
     3661 aattggagat caatatgctg atttgttttt ggcagctaag aatttatcag atgctatttt
     3721 actttcagat atcctaagag taaatactga aataactaag gctcccctat cagcttcaat
     3781 gattaaacgc tacgatgaac atcatcaaga cttgactctt ttaaaagctt tagttcgaca
     3841 acaacttcca gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg
     3901 ttatattgat gggggagcta gccaagaaga attttataaa tttatcaaac caattttaga
     3961 aaaaatggat ggtactgagg aattattggt gaaactaaat cgtgaagatt tgctgcgcaa
     4021 gcaacggacc tttgacaacg gctctattcc ccatcaaatt cacttgggtg agctgcatgc
     4081 tattttgaga agacaagaag acttttatcc atttttaaaa gacaatcgtg agaagattga
     4141 aaaaatcttg acttttcgaa ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg
     4201 ttttgcatgg atgactcgga agtctgaaga aacaattacc ccatggaatt ttgaagaagt
     4261 tgtcgataaa ggtgcttcag ctcaatcatt tattgaacgc atgacaaact ttgataaaaa
     4321 tcttccaaat gaaaaagtac taccaaaaca tagtttgctt tatgagtatt ttacggttta
     4381 taacgaattg acaaaggtca aatatgttac tgaaggaatg cgaaaaccag catttctttc
     4441 aggtgaacag aagaaagcca ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt
     4501 taagcaatta aaagaagatt atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc
     4561 aggagttgaa gatagattta atgcttcatt aggtacctac catgatttgc taaaaattat
     4621 taaagataaa gattttttgg ataatgaaga aaatgaagat atcttagagg atattgtttt
     4681 aacattgacc ttatttgaag atagggagat gattgaggaa agacttaaaa catatgctca
     4741 cctctttgat gataaggtga tgaaacagct taaacgtcgc cgttatactg gttggggacg
     4801 tttgtctcga aaattgatta atggtattag ggataagcaa tctggcaaaa caatattaga
     4861 ttttttgaaa tcagatggtt ttgccaatcg caattttatg cagctgatcc atgatgatag
     4921 tttgacattt aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca
     4981 tgaacatatt gcaaatttag ctggtagccc tgctattaaa aaaggtattt tacagactgt
     5041 aaaagttgtt gatgaattgg tcaaagtaat ggggcggcat aagccagaaa atatcgttat
     5101 tgaaatggca cgtgaaaatc agacaactca aaagggccag aaaaattcgc gagagcgtat
     5161 gaaacgaatc gaagaaggta tcaaagaatt aggaagtcag attcttaaag agcatcctgt
     5221 tgaaaatact caattgcaaa atgaaaagct ctatctctat tatctccaaa atggaagaga
     5281 catgtatgtg gaccaagaat tagatattaa tcgtttaagt gattatgatg tcgatgccat
     5341 tgttccacaa agtttcctta aagacgattc aatagacaat aaggtcttaa cgcgttctga
     5401 taaaaatcgt ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa
     5461 ctattggaga caacttctaa acgccaagtt aatcactcaa cgtaagtttg ataatttaac
     5521 gaaagctgaa cgtggaggtt tgagtgaact tgataaagct ggttttatca aacgccaatt
     5581 ggttgaaact cgccaaatca ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac
     5641 taaatacgat gaaaatgata aacttattcg agaggttaaa gtgattacct taaaatctaa
     5701 attagtttct gacttccgaa aagatttcca attctataaa gtacgtgaga ttaacaatta
     5761 ccatcatgcc catgatgcgt atctaaatgc cgtcgttgga actgctttga ttaagaaata
     5821 tccaaaactt gaatcggagt ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat
     5881 gattgctaag tctgagcaag aaataggcaa agcaaccgca aaatatttct tttactctaa
     5941 tatcatgaac ttcttcaaaa cagaaattac acttgcaaat ggagagattc gcaaacgccc
     6001 tctaatcgaa actaatgggg aaactggaga aattgtctgg gataaagggc gagattttgc
     6061 cacagtgcgc aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa cagaagtcca
     6121 gacaggcgga ttctccaagg agtcaatttt accaaaaaga aattcggaca agcttattgc
     6181 tcgtaaaaaa gactgggatc caaaaaaata tggtggtttt gatagtccaa cggtagctta
     6241 ttcagtccta gtggttgcta aggtggaaaa agggaaatcg aagaagttaa aatccgttaa
     6301 agagttacta gggatcacaa ttctggaaag aagttccttt gaaaaaaatc cgattgactt
     6361 tttagaagct aaaggatata aggaagttaa aaaagactta atcattaaac tacctaaata
     6421 tagtcttttt gagttagaaa acggtcgtaa acggatgctg gctagtgccg gagaattaca
     6481 aaaaggaaat gagctggctc tgccaagcaa atatgtgaat tttttatatt tagctagtca
     6541 ttatgaaaag ttgaagggta gtccagaaga taacgaacaa aaacaattgt ttgtggagca
     6601 gcataagcat tatttagatg agattattga gcaaatcagt gaattttcta agcgtgttat
     6661 tttagcagat gccaatttag ataaagttct tagtgcatat aacaaacata gagacaaacc
     6721 aatacgtgaa caagcagaaa atattattca tttatttacg ttgacgaatc ttggagctcc
     6781 cgctgctttt aaatattttg atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga
     6841 agttttagat gccactctta tccatcaatc catcactggt ctttatgaaa cacgcattga
     6901 tttgagtcag ctaggaggtg actaataaaa gcttgatcag atctcagctt ttttttgaag
     6961 tgcctggcag taagttgaaa aaatttgaaa taaacttgga ctatgttaat aaaaaaatac
     7021 aaattaacga acaaaaagat ttgaattatt atataattta gttaaaagca attttaaatg
     7081 agggaggttt ccatatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     7141 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     7201 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     7261 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     7321 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     7381 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     7441 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     7501 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     7561 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     7621 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     7681 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     7741 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     7801 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     7861 agatcaccaa ggtagtcggc aaataatgac gtcgccggag cggtcgagtt ctggaccgac
     7921 cggctcgggt tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7981 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8041 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8101 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8161 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8221 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8281 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8341 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8401 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8461 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8521 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8581 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8641 ggccttttgc tcacatgttc tttcctgcac tagatccccg gcaagttcat ccttagggtt
     8701 ttcatatctc ttaaaaagtc aatttctgct tgattaattt cttgtggatt attgtttttg
     8761 cggtttttca ttttttatct ccttgagtat tgaatttttt cttatatata aatatatagc
     8821 aaaaactatt tttgccaact tttttacaaa aaatttttta ttaaaacact tagggctttg
     8881 ctaaattctc ttttaaaaga acttagtaaa gccctatgga tttaagaact gattatactt
     8941 acgtagtaaa aatactacag atattaatag tatagaccat atcaaaatca taatcaattt
     9001 atatttatct aaaattgtag tccatacaaa atacaaaaaa attaggtttt tctaaattct
     9061 attttaaaag aacttagtaa cacctaatct ctaaattact tacaaaattc taaaattaga
     9121 tttttagttc ttcatatttc tttaaaagtt ttttaagaaa atctttttga ttttcaaaaa
     9181 tttcatgcat cataaaacta gtaaatttgg attttttttt gtaaaaatca taactttctt
     9241 gggttttaag ttgaaatctt aatggtttta ttggattttg cctagatttt tttaacgccg
     9301 gactttcttt atcttttata aaattataag aatttttaat accattttct attaaataat
     9361 cttcttttat aagcccctct tgcagtgcag ttgctaatct taaatactta tacgcttgag
     9421 tttttgctac atcaaagtct tttataaaat caagaaaagt tttataacca tcaaatttat
     9481 aatattgatt atcttttatt tcttttaaaa ttttaatatt ttccaccctg tggaatattt
     9541 cttttttgaa attgtcattt aacttttcct ttagtgtctt gtaataagaa attttacttt
     9601 cattaatcat taaattttgt tcaacataag tatctatctt ttttgttaaa actttaacaa
     9661 cacgtttttt ttccataaaa attttccttt atttttgcaa cattttcctt aattgatata
     9721 attgttacta ttttagattt tttaatatcc acttaagaat taatccctca ctagaatatt
     9781 ttttattata gaattcatat acatgttttt ctaaatctat aaatttatca acaattgctt
     9841 tattataggg tgtatctatc ttttcttttc ttaataatcg tctcatattc tttaaataac
     9901 aacaaacact gccggtttta aacctaaatt ctatgtaata tatttttgaa aaagtatata
     9961 cattttcaac cccgtttatt tcatacttta caaaaacatt atttattggt tttctataac
    10021 catagtaaat gcctaaaaat ttgtccccgt ctttcatagg atatagataa agtttttgaa
    10081 tctctgattt atctaataat ttttcaaaaa aaattataca ccgcttttgt ttgttactaa
    10141 ctactctatg caatgccttt aatatttttg tgtgatatat atttttatca cctttttttt
    10201 ctttcagcag gaagatactt tcttttggaa atttaatttt ttgttcattt attcttgcag
    10261 ccttaagtcc gattttcata tttaatcctt atatagcctt tctatttaat tcttttaaac
    10321 tcaataaact tttttggttt ttcattagtt ctaataattc gcaataatat gtgcaaaaca
    10381 ctttattgta ttctattttc ttttggttat tcaaatattc ttttataatt ggctttagaa
    10441 gttcaatatt tacttctttt tttagttgtt caataaggat attgaaaatg tttgttttta
    10501 aatcttgata attttgttga gaattttctt tttttctttc aattgacttc tctaatttac
    10561 gctttatatt gtttaaatcg ctatatttat gactttcaat aataaaatga ggcttaaatt
    10621 tgtaattttc gtatattttt tggaaacttg tttctaattg tttagaatta tattcatttt
    10681 tttcaaattc tttttgagtg ttgcatagaa tttcctttaa tttttcttgt ttttctttca
    10741 tacaagattt agtaaaatgt atatttttat tttttagtaa tttaatttct attattttta
    10801 tgattttaag tattttgatt atattatctt tatcaatatt taaatt
//
