LOCUS       sequence-308850-       11341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_Psyn-sgRNA500
FEATURES             Location/Qualifiers
     source          1..11341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..55
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        572..647
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(2090..2104,2105..2110,2111..2127,2128..2134)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2142..2158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2177..2188
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2195..2221,2222..2224,2225..4711,4712..4714,
                     4715..6301)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6471..7262
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7385..7973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7874..7893
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccggaatt ctaaagatct ttgacagcta gctcagtcct aggtataata ctagtggaag
       61 agccaaagtc aacacttata gacaaatatg atctttctaa ccttcacgaa atagcatcag
      121 ggggagcacc tttgtctaag gaagtaggag aggcagttgc aaagaggttt catttacccg
      181 gaattaggca aggatatggg ttaacagaaa ccacatctgc aattttaatt acacctgaag
      241 gagatgataa accaggagct gttgggaagg ttgttccttt ttttgaggct aaagttgttg
      301 atttagatac tggaaagact ttaggagtta atcaaagagg cgagttatgt gtaagaggac
      361 ctatgataat gtcaggatac gtaaataatc cagaagctac aaatgcattg atagataaag
      421 atggttggtt acactcaggc gatatagctt attgggacga agatgaacac ttttttatag
      481 ttgatagatt gaaatctttg attaagtata aaggttatca agtagctcca gcagaattgg
      541 aaagtatttt attgcaacac ccagctcttc cgttttagag ctagaaatag caagttaaaa
      601 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttggcgcg
      661 ccaaataatg cccgtgtccc tgttgaatag ggacacggtt ttttaacaat ttttttattc
      721 ctaggcctta gtggccggta cccggggatc cttattactg gccgctttct agcctggata
      781 cttgtctggc tagttgcatt agagagtcag ctagagccct agggctagct gtttgtgtat
      841 tcggagccag tgtagttttt ctttttacta acgatacggg taataattga ttgcctttta
      901 cagcctgacc ttgagacaat tgcaaaagtc tgtccacaga ggtttgacct aataatctaa
      961 aatcttgttt gatagtagta agtggaggta tgtagcaact agaatcttct gtatcatcat
     1021 aacccacaac tgaaatatca gccccaactc taagtccact ttcagtaata gctcgcatag
     1081 ctcctaaagc catttgatca ttagcaacta acatagcagt agggacaatg ccttcattta
     1141 acatttgcat tgtttgctga aatcctgaca tagcagacca atctccttct ctttctgcta
     1201 ttggttgtat ttgatttctt gttaaatact tgtgccatcc tgctaatctc aatctggcac
     1261 ttactgaact taagggacct gcaagtagtg caatttgttg atggcctaat gcaacaaggt
     1321 gttcaactcc taaccttgtt ccatcttcgt gagaaaaaat tatagagttt attggggttt
     1381 ggtccgacac atccaaaaac aatgctggaa cattagtaca tgctgcttca actgcaatcg
     1441 catcttggtc atcaagagga taattaatta taagtccact aacacgttga gctaataaat
     1501 tatgtactgc tgccttacaa gcctctacac ctgatctctc aaccattgat acaacaacgc
     1561 ttgcaccaag ttgatcagct ctcgatttaa ttgcggctac aatttgacta ggcgcatgta
     1621 atgcaagtga tgatgtggca actccaatta aaagtgattg tttccccgca agttgctgag
     1681 caacgcgatt tggtatataa ttcaactctg ccatagctgc ttcaaccttc tctcttgttt
     1741 ttgcagatac atgacttgct tggttcacaa ctctcgaaac cgtttggtag ctaaccccgg
     1801 catattcagc aacatcatac aaagtaacag gtttcatgga tcattcctcc atgataaaat
     1861 ttaaatttct gactttggca aatccttttg cctgttaaaa agaatagcac aaaaattaaa
     1921 aaaaaagaaa cgcaaatagc aatagcatta aaaataatta ttattattta tttctttgat
     1981 taaaatcaaa agcctttaat gtattttctt gatctttcat gcgcttaacg gtaaaatcca
     2041 aggtattaag ttcaccttcc acttttctct ctctatcttc tctagaaaat cataaaaaat
     2101 ttatttgctt tgtgagcgga taacaattat aatagattca attgtgagcg gataacaatt
     2161 tcacacagaa ttcattaaag aggagaaatt acatatggat aagaaatact caataggctt
     2221 agctatcggc acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc
     2281 taaaaagttc aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg
     2341 ggctctttta tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg
     2401 tagaaggtat acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga
     2461 gatggcgaaa gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga
     2521 agacaagaag catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca
     2581 tgagaaatat ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc
     2641 ggatttgcgc ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt
     2701 gattgaggga gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt
     2761 acaaacctac aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa
     2821 agcgattctt tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct
     2881 ccccggtgag aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac
     2941 ccctaatttt aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga
     3001 tacttacgat gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt
     3061 gtttttggca gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa
     3121 tactgaaata actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca
     3181 tcaagacttg actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga
     3241 aatctttttt gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca
     3301 agaagaattt tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt
     3361 attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc
     3421 tattccccat caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt
     3481 ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc
     3541 ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc
     3601 tgaagaaaca attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca
     3661 atcatttatt gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc
     3721 aaaacatagt ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata
     3781 tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt
     3841 tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt
     3901 caaaaaaata gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc
     3961 ttcattaggt acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa
     4021 tgaagaaaat gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag
     4081 ggagatgatt gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa
     4141 acagcttaaa cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg
     4201 tattagggat aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc
     4261 caatcgcaat tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca
     4321 aaaagcacaa gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg
     4381 tagccctgct attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa
     4441 agtaatgggg cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac
     4501 aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa
     4561 agaattagga agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga
     4621 aaagctctat ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga
     4681 tattaatcgt ttaagtgatt atgatgtcga tgccattgtt ccacaaagtt tccttaaaga
     4741 cgattcaata gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa
     4801 cgttccaagt gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc
     4861 caagttaatc actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag
     4921 tgaacttgat aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa
     4981 gcatgtggca caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact
     5041 tattcgagag gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga
     5101 tttccaattc tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct
     5161 aaatgccgtc gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt
     5221 ctatggtgat tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat
     5281 aggcaaagca accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga
     5341 aattacactt gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac
     5401 tggagaaatt gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat
     5461 gccccaagtc aatattgtca agaaaacaga agtccagaca ggcggattct ccaaggagtc
     5521 aattttacca aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa
     5581 aaaatatggt ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt
     5641 ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattct
     5701 ggaaagaagt tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga
     5761 agttaaaaaa gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg
     5821 tcgtaaacgg atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc
     5881 aagcaaatat gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc
     5941 agaagataac gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat
     6001 tattgagcaa atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa
     6061 agttcttagt gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat
     6121 tattcattta tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac
     6181 aacaattgat cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca
     6241 tcaatccatc actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgacta
     6301 ataaaagctt gatcagatct cagctttttt ttgaagtgcc tggcagtaag ttgaaaaaat
     6361 ttgaaataaa cttggactat gttaataaaa aaatacaaat taacgaacaa aaagatttga
     6421 attattatat aatttagtta aaagcaattt taaatgaggg aggtttccat atgagggaag
     6481 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     6541 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     6601 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     6661 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     6721 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     6781 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     6841 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     6901 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     6961 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     7021 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     7081 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     7141 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     7201 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     7261 aatgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc atgaccaaaa
     7321 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7381 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7441 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7501 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7561 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7621 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7681 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7741 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7801 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7861 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7921 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7981 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8041 ctgcactaga tccccggcaa gttcatcctt agggttttca tatctcttaa aaagtcaatt
     8101 tctgcttgat taatttcttg tggattattg tttttgcggt ttttcatttt ttatctcctt
     8161 gagtattgaa ttttttctta tatataaata tatagcaaaa actatttttg ccaacttttt
     8221 tacaaaaaat tttttattaa aacacttagg gctttgctaa attctctttt aaaagaactt
     8281 agtaaagccc tatggattta agaactgatt atacttacgt agtaaaaata ctacagatat
     8341 taatagtata gaccatatca aaatcataat caatttatat ttatctaaaa ttgtagtcca
     8401 tacaaaatac aaaaaaatta ggtttttcta aattctattt taaaagaact tagtaacacc
     8461 taatctctaa attacttaca aaattctaaa attagatttt tagttcttca tatttcttta
     8521 aaagtttttt aagaaaatct ttttgatttt caaaaatttc atgcatcata aaactagtaa
     8581 atttggattt ttttttgtaa aaatcataac tttcttgggt tttaagttga aatcttaatg
     8641 gttttattgg attttgccta gattttttta acgccggact ttctttatct tttataaaat
     8701 tataagaatt tttaatacca ttttctatta aataatcttc ttttataagc ccctcttgca
     8761 gtgcagttgc taatcttaaa tacttatacg cttgagtttt tgctacatca aagtctttta
     8821 taaaatcaag aaaagtttta taaccatcaa atttataata ttgattatct tttatttctt
     8881 ttaaaatttt aatattttcc accctgtgga atatttcttt tttgaaattg tcatttaact
     8941 tttcctttag tgtcttgtaa taagaaattt tactttcatt aatcattaaa ttttgttcaa
     9001 cataagtatc tatctttttt gttaaaactt taacaacacg ttttttttcc ataaaaattt
     9061 tcctttattt ttgcaacatt ttccttaatt gatataattg ttactatttt agatttttta
     9121 atatccactt aagaattaat ccctcactag aatatttttt attatagaat tcatatacat
     9181 gtttttctaa atctataaat ttatcaacaa ttgctttatt atagggtgta tctatctttt
     9241 cttttcttaa taatcgtctc atattcttta aataacaaca aacactgccg gttttaaacc
     9301 taaattctat gtaatatatt tttgaaaaag tatatacatt ttcaaccccg tttatttcat
     9361 actttacaaa aacattattt attggttttc tataaccata gtaaatgcct aaaaatttgt
     9421 ccccgtcttt cataggatat agataaagtt tttgaatctc tgatttatct aataattttt
     9481 caaaaaaaat tatacaccgc ttttgtttgt tactaactac tctatgcaat gcctttaata
     9541 tttttgtgtg atatatattt ttatcacctt ttttttcttt cagcaggaag atactttctt
     9601 ttggaaattt aattttttgt tcatttattc ttgcagcctt aagtccgatt ttcatattta
     9661 atccttatat agcctttcta tttaattctt ttaaactcaa taaacttttt tggtttttca
     9721 ttagttctaa taattcgcaa taatatgtgc aaaacacttt attgtattct attttctttt
     9781 ggttattcaa atattctttt ataattggct ttagaagttc aatatttact tcttttttta
     9841 gttgttcaat aaggatattg aaaatgtttg tttttaaatc ttgataattt tgttgagaat
     9901 tttctttttt tctttcaatt gacttctcta atttacgctt tatattgttt aaatcgctat
     9961 atttatgact ttcaataata aaatgaggct taaatttgta attttcgtat attttttgga
    10021 aacttgtttc taattgttta gaattatatt catttttttc aaattctttt tgagtgttgc
    10081 atagaatttc ctttaatttt tcttgttttt ctttcataca agatttagta aaatgtatat
    10141 ttttattttt tagtaattta atttctatta tttttatgat tttaagtatt ttgattatat
    10201 tatctttatc aatatttaaa ttgaataaaa ttgtaagaaa aattgaaaga atttttttac
    10261 ataaaaagtt acatttattg aagtaatttc ttacttgata cttttctatt tcattaatct
    10321 ttctttcttc ttttatatta tttttattat ttaaacactc ctttaaatct acactcccat
    10381 ttttagtaaa tctatcttta aggccccttg aaactctatt ttgaaatctt aaatcttttt
    10441 tttcttgaaa gtatttatag attttttggt aacattcttt ttttgaaaaa ttaagttgat
    10501 agtatatttc ggtccccaaa tttaccccta agtgtttgtg ataattagtt gtgactttaa
    10561 attctttttc taatttgtaa aggtaatttt gcagtgtttt tagtttaacg ggtgtttgac
    10621 tatttctttt aaggttttca ttaaagtaat agagtatgtt tttttgtgtg tatttcttat
    10681 atgttttatt tatgtacgct agtgttgaga taaggactac taatttatac tgtaatttat
    10741 tgtagcaagt tgtggtttta ctggttttgt ttatattaga taagttttcc atttatagct
    10801 ccttgaatac cggatttttt atctataaag tatatatatc acaattttga tttaaaagta
    10861 aattcttttt ttaaaaatac aaaaattttt tataaattat atagaccatg atattgtatt
    10921 aatactaatt gattttaatt taaggttgaa ctaaaataaa catagttttg tagaaaataa
    10981 tttttcatta ttttgctact tgatattgga tcgtaaaaat attagggctt tacaaagttc
    11041 ttttaaaaga gaatttagta aagccctaag tcttttaata aaaaattttt tgtaaaaaag
    11101 ttggcaaaaa tagtttttgc tatatattta tatataagaa aaaattcaat actcaaggag
    11161 ataaaaaatg gaaaacttaa acaataatcc acaaaaaatt aatcaaataa ataagcaaac
    11221 taattttatt ggttttgaaa attttataga acttgataat caagttaatc aaactaaaga
    11281 aaaaagtaaa ttaagtaaga taggtaagaa attacccgga ataagcagtc aagtctagtg
    11341 c
//
