LOCUS       sequence-308853-       11315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(RBSmut)_arr2
FEATURES             Location/Qualifiers
     source          1..11315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(3184..3198,3199..3204,3205..3221,3222..3228)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    3204..3220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3236..3252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(3289..3315,3316..3318,3319..5805,5806..5808,
                     5809..7395)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7565..8356
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8479..9067
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8968..8987
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgaataaaat tgtaagaaaa attgaaagaa tttttttaca taaaaagtta catttattga
       61 agtaatttct tacttgatac ttttctattt cattaatctt tctttcttct tttatattat
      121 ttttattatt taaacactcc tttaaatcta cactcccatt tttagtaaat ctatctttaa
      181 ggccccttga aactctattt tgaaatctta aatctttttt ttcttgaaag tatttataga
      241 ttttttggta acattctttt tttgaaaaat taagttgata gtatatttcg gtccccaaat
      301 ttacccctaa gtgtttgtga taattagttg tgactttaaa ttctttttct aatttgtaaa
      361 ggtaattttg cagtgttttt agtttaacgg gtgtttgact atttctttta aggttttcat
      421 taaagtaata gagtatgttt ttttgtgtgt atttcttata tgttttattt atgtacgcta
      481 gtgttgagat aaggactact aatttatact gtaatttatt gtagcaagtt gtggttttac
      541 tggttttgtt tatattagat aagttttcca tttatagctc cttgaatacc ggatttttta
      601 tctataaagt atatatatca caattttgat ttaaaagtaa attctttttt taaaaataca
      661 aaaatttttt ataaattata tagaccatga tattgtatta atactaattg attttaattt
      721 aaggttgaac taaaataaac atagttttgt agaaaataat ttttcattat tttgctactt
      781 gatattggat cgtaaaaata ttagggcttt acaaagttct tttaaaagag aatttagtaa
      841 agccctaagt cttttaataa aaaatttttt gtaaaaaagt tggcaaaaat agtttttgct
      901 atatatttat atataagaaa aaattcaata ctcaaggaga taaaaaatgg aaaacttaaa
      961 caataatcca caaaaaatta atcaaataaa taagcaaact aattttattg gttttgaaaa
     1021 ttttatagaa cttgataatc aagttaatca aactaaagaa aaaagtaaat taagtaagat
     1081 aggtaagaaa ttacccggaa taagcagtca agtctagtgc ggccgcatgg cttgttatga
     1141 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     1201 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     1261 aacaaagtta aacatcatga ggaggtaacg tcatggtaaa agattggatt cctatttctc
     1321 atgataatta taaacaagta caaggaccat tttatcatgg aactaaagct aatttagcaa
     1381 ttggtgattt actaactaca ggatttattt ctcattttga agatggtcga attcttaaac
     1441 atatttattt ttcagcttta atggaaccag cagtttgggg agctgaactt gctatgtcac
     1501 tatctggtct tgaaggtcgt ggttatatat atatagttga accaacagga ccatttgaag
     1561 atgatccaaa tcttacaaat aaaaaatttc ctggtaatcc aacacaatct tatagaactt
     1621 gtgaaccttt aagaattgtt ggtgttgttg aagattggga aggacatcct gttgaattaa
     1681 taagaggaat gttagattca ttagaagatt taaaacgtcg tggtttacat gttattgaag
     1741 attaacctgg cgcgccaaat aatgcccgtg tccctgttga atagggacac ggttttttaa
     1801 caattttttt attcctaggc cttagtggcc ggtacccggg gatccttatt actggccgct
     1861 ttctagcctg gatacttgtc tggctagttg cattagagag tcagctagag ccctagggct
     1921 agctgtttgt gtattcggag ccagtgtagt ttttcttttt actaacgata cgggtaataa
     1981 ttgattgcct tttacagcct gaccttgaga caattgcaaa agtctgtcca cagaggtttg
     2041 acctaataat ctaaaatctt gtttgatagt agtaagtgga ggtatgtagc aactagaatc
     2101 ttctgtatca tcataaccca caactgaaat atcagcccca actctaagtc cactttcagt
     2161 aatagctcgc atagctccta aagccatttg atcattagca actaacatag cagtagggac
     2221 aatgccttca tttaacattt gcattgtttg ctgaaatcct gacatagcag accaatctcc
     2281 ttctctttct gctattggtt gtatttgatt tcttgttaaa tacttgtgcc atcctgctaa
     2341 tctcaatctg gcacttactg aacttaaggg acctgcaagt agtgcaattt gttgatggcc
     2401 taatgcaaca aggtgttcaa ctcctaacct tgttccatct tcgtgagaaa aaattataga
     2461 gtttattggg gtttggtccg acacatccaa aaacaatgct ggaacattag tacatgctgc
     2521 ttcaactgca atcgcatctt ggtcatcaag aggataatta attataagtc cactaacacg
     2581 ttgagctaat aaattatgta ctgctgcctt acaagcctct acacctgatc tctcaaccat
     2641 tgatacaaca acgcttgcac caagttgatc agctctcgat ttaattgcgg ctacaatttg
     2701 actaggcgca tgtaatgcaa gtgatgatgt ggcaactcca attaaaagtg attgtttccc
     2761 cgcaagttgc tgagcaacgc gatttggtat ataattcaac tctgccatag ctgcttcaac
     2821 cttctctctt gtttttgcag atacatgact tgcttggttc acaactctcg aaaccgtttg
     2881 gtagctaacc ccggcatatt cagcaacatc atacaaagta acaggtttca tggatcattc
     2941 ctccatgata aaatttaaat ttctgacttt ggcaaatcct tttgcctgtt aaaaagaata
     3001 gcacaaaaat taaaaaaaaa gaaacgcaaa tagcaatagc attaaaaata attattatta
     3061 tttatttctt tgattaaaat caaaagcctt taatgtattt tcttgatctt tcatgcgctt
     3121 aacggtaaaa tccaaggtat taagttcacc ttccactttt ctctctctat cttctctaga
     3181 aaatcataaa aaatttattt gctttgtgag cggataacaa ttataataga ttcaattgtg
     3241 agcggataac aatttcacac agaattcatt aaagaagaga aattacatat ggataagaaa
     3301 tactcaatag gcttagctat cggcacaaat agcgtcggat gggcggtgat cactgatgaa
     3361 tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa
     3421 aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc gactcgtctc
     3481 aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag
     3541 attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct
     3601 tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat
     3661 gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat
     3721 tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt
     3781 cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta
     3841 tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt
     3901 ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaaaat
     3961 ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct cattgctttg
     4021 tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga tgctaaatta
     4081 cagctttcaa aagatactta cgatgatgat ttagataatt tattggcgca aattggagat
     4141 caatatgctg atttgttttt ggcagctaag aatttatcag atgctatttt actttcagat
     4201 atcctaagag taaatactga aataactaag gctcccctat cagcttcaat gattaaacgc
     4261 tacgatgaac atcatcaaga cttgactctt ttaaaagctt tagttcgaca acaacttcca
     4321 gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat
     4381 gggggagcta gccaagaaga attttataaa tttatcaaac caattttaga aaaaatggat
     4441 ggtactgagg aattattggt gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc
     4501 tttgacaacg gctctattcc ccatcaaatt cacttgggtg agctgcatgc tattttgaga
     4561 agacaagaag acttttatcc atttttaaaa gacaatcgtg agaagattga aaaaatcttg
     4621 acttttcgaa ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg
     4681 atgactcgga agtctgaaga aacaattacc ccatggaatt ttgaagaagt tgtcgataaa
     4741 ggtgcttcag ctcaatcatt tattgaacgc atgacaaact ttgataaaaa tcttccaaat
     4801 gaaaaagtac taccaaaaca tagtttgctt tatgagtatt ttacggttta taacgaattg
     4861 acaaaggtca aatatgttac tgaaggaatg cgaaaaccag catttctttc aggtgaacag
     4921 aagaaagcca ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt taagcaatta
     4981 aaagaagatt atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc aggagttgaa
     5041 gatagattta atgcttcatt aggtacctac catgatttgc taaaaattat taaagataaa
     5101 gattttttgg ataatgaaga aaatgaagat atcttagagg atattgtttt aacattgacc
     5161 ttatttgaag atagggagat gattgaggaa agacttaaaa catatgctca cctctttgat
     5221 gataaggtga tgaaacagct taaacgtcgc cgttatactg gttggggacg tttgtctcga
     5281 aaattgatta atggtattag ggataagcaa tctggcaaaa caatattaga ttttttgaaa
     5341 tcagatggtt ttgccaatcg caattttatg cagctgatcc atgatgatag tttgacattt
     5401 aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca tgaacatatt
     5461 gcaaatttag ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt
     5521 gatgaattgg tcaaagtaat ggggcggcat aagccagaaa atatcgttat tgaaatggca
     5581 cgtgaaaatc agacaactca aaagggccag aaaaattcgc gagagcgtat gaaacgaatc
     5641 gaagaaggta tcaaagaatt aggaagtcag attcttaaag agcatcctgt tgaaaatact
     5701 caattgcaaa atgaaaagct ctatctctat tatctccaaa atggaagaga catgtatgtg
     5761 gaccaagaat tagatattaa tcgtttaagt gattatgatg tcgatgccat tgttccacaa
     5821 agtttcctta aagacgattc aatagacaat aaggtcttaa cgcgttctga taaaaatcgt
     5881 ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga
     5941 caacttctaa acgccaagtt aatcactcaa cgtaagtttg ataatttaac gaaagctgaa
     6001 cgtggaggtt tgagtgaact tgataaagct ggttttatca aacgccaatt ggttgaaact
     6061 cgccaaatca ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac taaatacgat
     6121 gaaaatgata aacttattcg agaggttaaa gtgattacct taaaatctaa attagtttct
     6181 gacttccgaa aagatttcca attctataaa gtacgtgaga ttaacaatta ccatcatgcc
     6241 catgatgcgt atctaaatgc cgtcgttgga actgctttga ttaagaaata tccaaaactt
     6301 gaatcggagt ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat gattgctaag
     6361 tctgagcaag aaataggcaa agcaaccgca aaatatttct tttactctaa tatcatgaac
     6421 ttcttcaaaa cagaaattac acttgcaaat ggagagattc gcaaacgccc tctaatcgaa
     6481 actaatgggg aaactggaga aattgtctgg gataaagggc gagattttgc cacagtgcgc
     6541 aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa cagaagtcca gacaggcgga
     6601 ttctccaagg agtcaatttt accaaaaaga aattcggaca agcttattgc tcgtaaaaaa
     6661 gactgggatc caaaaaaata tggtggtttt gatagtccaa cggtagctta ttcagtccta
     6721 gtggttgcta aggtggaaaa agggaaatcg aagaagttaa aatccgttaa agagttacta
     6781 gggatcacaa ttctggaaag aagttccttt gaaaaaaatc cgattgactt tttagaagct
     6841 aaaggatata aggaagttaa aaaagactta atcattaaac tacctaaata tagtcttttt
     6901 gagttagaaa acggtcgtaa acggatgctg gctagtgccg gagaattaca aaaaggaaat
     6961 gagctggctc tgccaagcaa atatgtgaat tttttatatt tagctagtca ttatgaaaag
     7021 ttgaagggta gtccagaaga taacgaacaa aaacaattgt ttgtggagca gcataagcat
     7081 tatttagatg agattattga gcaaatcagt gaattttcta agcgtgttat tttagcagat
     7141 gccaatttag ataaagttct tagtgcatat aacaaacata gagacaaacc aatacgtgaa
     7201 caagcagaaa atattattca tttatttacg ttgacgaatc ttggagctcc cgctgctttt
     7261 aaatattttg atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga agttttagat
     7321 gccactctta tccatcaatc catcactggt ctttatgaaa cacgcattga tttgagtcag
     7381 ctaggaggtg actaataaaa gcttgatcag atctcagctt ttttttgaag tgcctggcag
     7441 taagttgaaa aaatttgaaa taaacttgga ctatgttaat aaaaaaatac aaattaacga
     7501 acaaaaagat ttgaattatt atataattta gttaaaagca attttaaatg agggaggttt
     7561 ccatatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     7621 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     7681 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     7741 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     7801 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     7861 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     7921 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     7981 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     8041 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     8101 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     8161 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     8221 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     8281 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     8341 ggtagtcggc aaataatgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     8401 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8461 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8521 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8581 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8641 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8701 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8761 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8821 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8881 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8941 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9001 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9061 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9121 tcacatgttc tttcctgcac tagatccccg gcaagttcat ccttagggtt ttcatatctc
     9181 ttaaaaagtc aatttctgct tgattaattt cttgtggatt attgtttttg cggtttttca
     9241 ttttttatct ccttgagtat tgaatttttt cttatatata aatatatagc aaaaactatt
     9301 tttgccaact tttttacaaa aaatttttta ttaaaacact tagggctttg ctaaattctc
     9361 ttttaaaaga acttagtaaa gccctatgga tttaagaact gattatactt acgtagtaaa
     9421 aatactacag atattaatag tatagaccat atcaaaatca taatcaattt atatttatct
     9481 aaaattgtag tccatacaaa atacaaaaaa attaggtttt tctaaattct attttaaaag
     9541 aacttagtaa cacctaatct ctaaattact tacaaaattc taaaattaga tttttagttc
     9601 ttcatatttc tttaaaagtt ttttaagaaa atctttttga ttttcaaaaa tttcatgcat
     9661 cataaaacta gtaaatttgg attttttttt gtaaaaatca taactttctt gggttttaag
     9721 ttgaaatctt aatggtttta ttggattttg cctagatttt tttaacgccg gactttcttt
     9781 atcttttata aaattataag aatttttaat accattttct attaaataat cttcttttat
     9841 aagcccctct tgcagtgcag ttgctaatct taaatactta tacgcttgag tttttgctac
     9901 atcaaagtct tttataaaat caagaaaagt tttataacca tcaaatttat aatattgatt
     9961 atcttttatt tcttttaaaa ttttaatatt ttccaccctg tggaatattt cttttttgaa
    10021 attgtcattt aacttttcct ttagtgtctt gtaataagaa attttacttt cattaatcat
    10081 taaattttgt tcaacataag tatctatctt ttttgttaaa actttaacaa cacgtttttt
    10141 ttccataaaa attttccttt atttttgcaa cattttcctt aattgatata attgttacta
    10201 ttttagattt tttaatatcc acttaagaat taatccctca ctagaatatt ttttattata
    10261 gaattcatat acatgttttt ctaaatctat aaatttatca acaattgctt tattataggg
    10321 tgtatctatc ttttcttttc ttaataatcg tctcatattc tttaaataac aacaaacact
    10381 gccggtttta aacctaaatt ctatgtaata tatttttgaa aaagtatata cattttcaac
    10441 cccrtttatt tcatacttta caaaaacatt atttattggt tttctataac catagtaaat
    10501 gcctaaaaat ttgtccccgt ctttcatagg atatagataa agtttttgaa tctctgattt
    10561 atctaataat ttttcaaaaa aaattataca ccgcttttgt ttgttactaa ctactctatg
    10621 caatgccttt aatatttttg tgtgatatat atttttatca cctttttttt ctttcagcag
    10681 gaagatactt tcttttggaa atttaatttt ttgttcattt attcttgcag ccttaagtcc
    10741 gattttcata tttaatcctt atatagcctt tctatttaat tcttttaaac tcaataaact
    10801 tttttggttt ttcattagtt ctaataattc gcaataatat gtgcaaaaca ctttattgta
    10861 ttctattttc ttttggttat tcaaatattc ttttataatt ggctttagaa gttcaatatt
    10921 tacttctttt tttagttgtt caataaggat attgaaaatg tttgttttta aatcttgata
    10981 attttgttga gaattttctt tttttctttc aattgacttc tctaatttac gctttatatt
    11041 gtttaaatcg ctatatttat gactttcaat aataaaatga ggcttaaatt tgtaattttc
    11101 gtatattttt tggaaacttg tttctaattg tttagaatta tattcatttt tttcaaattc
    11161 tttttgagtg ttgcatagaa tttcctttaa tttttcttgt ttttctttca tacaagattt
    11221 agtaaaatgt atatttttat tttttagtaa tttaatttct attattttta tgattttaag
    11281 tattttgatt atattatctt tatcaatatt taaat
//
