LOCUS       sequence-308859-       10658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning 
            homology arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 10658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1582
FEATURES             Location/Qualifiers
     source          1..10658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(154..171)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(154..170)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(163..185)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(379..398)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     498..520
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(558..576)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        644..748
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(749..817,818..1609)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(967..986)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1780..2368
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2269..2288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2522..2539
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2656..2677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2692..2697,2698..2715,2716..2722)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2730..2746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2735..2757
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2754..2770
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2754..2770
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3042..3347
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(3395..3418)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3422..3445)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3449..3472)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3500..3631)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     protein_bind    3677..3710
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4063..4087
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4088..5113)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(4088..5113)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(5116..5140)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      7058..7381
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(9198..9227)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    9237..9270
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(9288..9308)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      9854..9863
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(10241..10546)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
ORIGIN
        1 ggcttcattc tgcatggttg tgcttaccga accggagata ttgacatcat atatgccttg
       61 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc
      121 tttttgacat acttcgggta actagtgccg gcgactggcc gtcgttttac aacgtcgtga
      181 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      241 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      301 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      361 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
      421 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
      481 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
      541 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      601 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      661 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      721 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      781 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      841 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      901 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      961 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     1021 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1081 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1141 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1201 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1261 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1321 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1381 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1441 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1501 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1561 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1621 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1681 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1741 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1801 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1861 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1921 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1981 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2041 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2101 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2161 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2221 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2281 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2341 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2401 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2461 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2521 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     2581 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     2641 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     2701 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     2761 cagctatgac catgttatcg attcctagga acccgaagta tgtcaaaaag aggtatgcta
     2821 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata
     2881 tgatgtcaat atctccggtt cggtaagcac aaccatgcag aatgaagccc gtcgtctgcg
     2941 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga
     3001 aatgaacggc tcttttgccg acgagaacag gggctggtga aatgcagttt aaggtttaca
     3061 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
     3121 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtcc
     3181 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
     3241 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg
     3301 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
     3361 ttatacacag ccagtctgca ggtcgaacta gtctcttgtc atcgtcatcc ttgtaatcac
     3421 gcttgtcgtc gtcgtccttg tagtcacgct tatcgtcatc gtctttataa tcagatcctg
     3481 agccagaacc gccaccaccc ttcacgaacg ccgccgcctc cgggccaccg cttgattttt
     3541 ggcaggaaac catcacgttc ttccggtatg atctcaccgg tggccatccc acaacttgtg
     3601 ccttggccgg aggtttggct ggatctttag gcatcccgcc acctccggat ccgtaatcag
     3661 atccggctcc ctgaaaataa cttcgtataa tgtatgctat acgaagttat taaaaattgc
     3721 ggtcataaac tgaaacgtaa catatgataa ggtattttgt gtgcggtttt ttctatgatg
     3781 tttcaaaaaa atttatcaga agtaaaaaac ataagaagtg tacacagaac attgtgtaga
     3841 ttttgggata gaattattat taatgggggg agggagcaca atttttttcg actttttatt
     3901 acgaattaat tctatttttg aatctacaca atttcattgt tagaggtgac ttaaaagaag
     3961 ctaaaaaaca aagaaattaa gagaagtttt ttgataataa caaaaatagg gggtgggagc
     4021 acagggagaa agagcatgta gggatgttga agagtaattg gacccacttt gtacaagaaa
     4081 gctgggtcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     4141 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     4201 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     4261 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     4321 acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     4381 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     4441 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     4501 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     4561 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     4621 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     4681 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     4741 ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt
     4801 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
     4861 cgctgaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     4921 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     4981 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     5041 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     5101 caggcttttt catggagcct gcttttttgt acaaacttgc ggcaccgcct gacatattac
     5161 cttaaaattc aaaaattaat ttcagatcag tcataaccaa gaaaacaaaa taaacgttag
     5221 gacagaaaat tcaaattaat taaaaattat gctgaataag aaaattacat gcaaagcctt
     5281 attttcctca tagaaacagt tgaataacat attttttaat tgaaaacata gcaaaattcg
     5341 tggtaaaatt tgtcaaaacc ttggaaaaaa tcgaaaaaag aacatttccc gatgcatttt
     5401 cctatttctc gttgccgaca acgtataaac gttgaatact cagtcaacga atttggctgc
     5461 tggcttggac tctcgcacgg aagagacgcg tgtgtagatt tacgacgaaa gcaaaaatga
     5521 tcaccaaaaa cggaacgttg agctggacgg aaatagtggt aaagtgacat gattatagtt
     5581 tgaagatttc taatttcaca attagagcaa atgttgttcg gtatttattt tcaacggtat
     5641 ttatactatt ttccaccttt ttctagaaca ttcgagctgc ttgttgcaaa aggagggcga
     5701 ctcacattcg gtacatggaa aagtagtgta cacaataaag agacccagat acattttccg
     5761 tctgcgtctc tttgcaccca ccgggagtat tttcaaacga atgcatctag gaccttctag
     5821 aacattctgt aaggctgcag aatgcgggta tataaggaaa gcgggctcag aggaagccaa
     5881 cacgctttgt tctagtgcat ctaaaaaact tcgaaaatat gtcaaatctt ctcactgttc
     5941 atcaaaatct tccagcactt ccagttgacg cgacaagtga tgaagttcgc aagaacctca
     6001 tggatatgtt ccgtgacaga caagccttct ccgagcacac ctggaagatg cttctctcag
     6061 tgtgccgttc ttgggctgcc tggtgcaagc ttaacaaccg caagtggttc ccagccgagc
     6121 cagaagacgt tcgtgactac ttgctctacc tccaagctcg tggattggcg gtgaagacga
     6181 tccaacaaca tctcggacag ctcaatatgc tccatcgccg atctggactt ccacgcccat
     6241 cagattctaa tgccgtatct ctcgtaatga gacgtatccg caaggaaaat gttgatgctg
     6301 gagagagagc caagcaagcc ctcgccttcg aaaggtaagt ttaaatatta atgtattcaa
     6361 tcctaagact tgacgaaatt ttcagaaccg acttcgatca ggtccgttct cttatggaaa
     6421 attccgatcg ttgccaggac atccgaaacc tcgccttcct cggaattgct tacaacacac
     6481 ttctccgtat cgccgaaatt gcccgcattc gcgtcaagga tattagtcgc acagacggag
     6541 gaagaatgct catccacatc ggacgtacaa aaacgctggt ctcgactgcc ggagtcgaaa
     6601 aagcgctctc ccttggagtt accaagctcg ttgagcgctg gatttcggtt tccggagtcg
     6661 ccgatgatcc aaacaactac ctgttctgtc gtgttcgcaa gaacggagtt gctgccccga
     6721 gtgcaacatc acaactctcg actcgagctc ttgaaggtaa gtttaaacaa catattagct
     6781 aagaataagc tgaaaataaa ttttcaggaa tcttcgaagc tacccaccgt ctcatctacg
     6841 gagccaagga tgattccgga caacgttact tggcttggtc tggacattcc gcccgtgtcg
     6901 gagctgcccg tgacatggct cgcgctggag tttccatccc agagattatg caagccggag
     6961 gttggaccaa cgtcaacatt gtgatgaact atattagaaa cttggattct gaaactggcg
     7021 ccatggttcg tcttctcgaa gacggtgatt aagataaatg caaaatcctt tcaagcattc
     7081 ccttcttctc tatcactctt ctttcttttt gtcaaaaaat tctctcgcta atttatttgc
     7141 ttttttaatg ttattatttt atgacttttt atagtcactg aaaagtttgc atctgagtga
     7201 agtgaatgct atcaaaatgt gattctgtct gatgtacttt cacaatctct cttcaattcc
     7261 attttgaagt gctttaaacc cgaaaggttg agaaaaatgc gagcgctcaa atatttgtat
     7321 tgtgttcgtt gagtgaccca acaaaaagag gaaactttat tgtgccgcca agaaaaaagt
     7381 ctcactgaat ctcaaatatt ttattagaaa acaccaacaa tttagatatt tctgtatcca
     7441 cggttggcgt tgacagcctc tgatggtgga gacttgtctg ggcactggca gtactcggca
     7501 tcctttcctg gtcttccgtc tggtccagtc tctccctggg ttccgtctgg tccttgacgt
     7561 ccttgctttc cagatggtcc ttctggtcca acggttcctg gctctcctgg tggtccgtga
     7621 agaccgttct gtccagctgg tcctgttggt ccttgatctc ctcttggtcc ctttggtcct
     7681 ggtcttccga ttggatgctc ggcgtcgcgt cccttctctc ctggtggtcc tggctctccg
     7741 atctttccga tttgaccgga tgatccttgc tctcctggat gtcctggttg tccatctctt
     7801 cctgggtttc cattttgtcc tcttggtcct ggaagtccac gtggtcctgg tcttccaaga
     7861 gctcctggtg gtccaactgg tccttgtggg caagtgaagc atccgacaga ctcgcgttgt
     7921 ggagcgatat catcagctcc aactcctgga acaccgtcaa gtcctggaac tccgtcgagt
     7981 cctggaactc ctggaactcc ctttggtccg gatggtccag ctgggcactt gttatcagca
     8041 ttgcagttgc aggttggtcc atttccattg gctccatttg gagtctttgg ctggttgaag
     8101 actggtggaa ctgctggtgg ttgacctggt ggagcagatg gggatccatc agcatgtgga
     8161 gttggggtag cgttggtctc ttcatattga caacggacac gcttgctaga tcttccgatg
     8221 acaactatgt ccttccacaa tccattggta gattgctaga aaattaaaaa ttatttaaaa
     8281 ttcaaaatat aatttaactc actctgaaca cattcatctc ggtatcaagc ttctctctga
     8341 atccatcaac ctccgagtag atggttgaca tggtcatgaa gcataccata agagtggcga
     8401 cagtgaccga agccgtcaca taacacgcaa gttttacaga catctctgaa attatcaaat
     8461 ttaggatttc aaaataattc ggccaaagaa atgtaactca cctagtcttc gagaagcaga
     8521 gacttgtcga tttcctaccg gattcgtctc cttttattag gaaatctttc cgcaagtttt
     8581 caagtttttt ttgttttttt tttacattac cgacgagaca gtgtgcgaga cgcagagacg
     8641 ataaaagact ggataagaac gggagataag gagtacggat gtaggtgagg aatgtggccg
     8701 aaaggacaga tgggctttat tcgggaagat tggttgggaa atgaaccaga gaagaatgac
     8761 tgaataaagt tgttgaaact tggtgagaat ggaagataga caatcgaggg cagctagttt
     8821 ccttcctcct ctatatattt ttatgttttc ttttaaacct tcctccatat ttctgaattg
     8881 aatgttgttg catattgtgt gtccttattt catttccatc atccttttgt gtgtttcccc
     8941 cttccaccaa ttccccccac gtcctaatga aatgaaagcg agaagataaa taaacttgca
     9001 cattgtgttc agatgagagg agcggaatac gaattgggag acggaaagag aaaaaaaaat
     9061 gtttagattt ggattgaatt aattttttag aaagcaagaa aaaaaacaca aagagaataa
     9121 ataaataata ggaaaaaaaa caaatattca gtatttaaaa tttgaaaatt caacgacgtt
     9181 ggcgtcgatc atccttagag gtcctcctcg gagataagct tttgctctcc ctgaaaataa
     9241 cttcgtataa tgtatgctat acgaagttat tttaaactta cctttccgga ttgaaagtac
     9301 agattctcct tgtagagctc gtccattccc atgacgtcgg tgaaggcctt ttgccactcc
     9361 ttgaagttga gctcggtctt ggagtgcttg agctcggtct tacggaagac gtacattggt
     9421 tggttcttga ggtagttggc ggccattggc ttggcgaagg tgtaggtggt acgggcggtg
     9481 gaacggtaac gctttccgtt tccggtggtg taggaccact tgaaggtgga gatgatggtc
     9541 tgaaaattta aatcagatta gttagttagt aaaatcatgt ttaaacttac cttgtcgttt
     9601 gggtaggtct tcttggaacg gcaccagtcg gcggcggtga gggagttggt catgactggt
     9661 ccgtcggctg ggaatccggt tcccttgact tgggcctctc cctgaaaatt taaatatgta
     9721 tggttagtta gtaccgaact gtttaaactt accttgatgt gggatccctc gtaggtgtaa
     9781 cggtagttga cggtgaggga ggctccgtcc tcgaattgca tggtacggtg gacttggtat
     9841 ccggatccgt cgaccatggc ggcttggaat ggggacattc cgtctgggta tgggaggtat
     9901 tggtggaatc cgtatccgat gtgtgggacg aggatccatg gggagaattg gaggtctccc
     9961 tgaaaattta aataatcagg gttagttagt atatatatgt ttaaacttac cttggtggac
    10021 ttgaggttga gctcctcgta tccgtcgttt gggtttccgg ttccttgtcc gaccatgtcg
    10081 aagtcgactc cgttgatgga tccgaagatg tggagctcgt gggtggctgg gagggaggcc
    10141 atgttgtcct cctctccctt ggagaccatc gatgctcctg aggctcccga tgctccctag
    10201 gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca
    10261 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
    10321 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
    10381 tatgcaccac cgggtaaagt tcacggggga ctttatctga cagcagacgt gcactggcca
    10441 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
    10501 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg
    10561 ttctcgtcgg caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
    10621 tgattttccg ctttccagcg ttcggcacgc agacgacg
//
