LOCUS       sequence-308867-       10535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-to-T base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10535)
  AUTHORS   Arbab M, Shen MW, Mok B, Wilson C, Matuszek Z, Cassa CA, Liu DR
  TITLE     Determinants of Base Editing Outcomes from Target Library Analysis 
            and Machine Learning.
  JOURNAL   Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 
            10.1016/j.cell.2020.05.037.
  PUBMED    32533916
REFERENCE   2  (bases 1 to 10535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037."
COMMENT     Sequence Label: p2T-CMV-BE4maxCP1028-BlastR
FEATURES             Location/Qualifiers
     source          1..10535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             116..136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             137..820
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2000..3718
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5111..5359
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5390..5638
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5681..5701
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5710..5727
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5750..5767)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5756..5980
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6051..6067)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6051..6067)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6064..6086)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6075..6091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6099..6105,6106..6123,6124..6129))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6144..6165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    6282..6403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6293..6312)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6347..6366
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6561..6959)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6978..7025)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7073..7402)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7087..7222
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7141..7160)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7387..7407
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(7526..7713,7714..7725)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(7786..8374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7866..7885)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8545..9336,9337..9405))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9168..9187
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9406..9510)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9589..9608)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9800..9811,9812..9949)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        9958..10337
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(10338..10535,1..6)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10492..10512
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
       61 tatagggaga gccgccacca tgaaacggac agccgacgga agcgagttcg agtcaccaaa
      121 gaagaagcgg aaagtctcct cagagactgg gcctgtcgcc gtcgatccaa ccctgcgccg
      181 ccggattgaa cctcacgagt ttgaagtgtt ctttgacccc cgggagctga gaaaggagac
      241 atgcctgctg tacgagatca actggggagg caggcactcc atctggaggc acacctctca
      301 gaacacaaat aagcacgtgg aggtgaactt catcgagaag tttaccacag agcggtactt
      361 ctgccccaat accagatgta gcatcacatg gtttctgagc tggtcccctt gcggagagtg
      421 tagcagggcc atcaccgagt tcctgtccag atatccacac gtgacactgt ttatctacat
      481 cgccaggctg tatcaccacg cagacccaag gaataggcag ggcctgcgcg atctgatcag
      541 ctccggcgtg accatccaga tcatgacaga gcaggagtcc ggctactgct ggcggaactt
      601 cgtgaattat tctcctagca acgaggccca ctggcctagg tacccacacc tgtgggtgcg
      661 cctgtacgtg ctggagctgt attgcatcat cctgggcctg cccccttgtc tgaatatcct
      721 gcggagaaag cagccccagc tgaccttctt tacaatcgcc ctgcagtctt gtcactatca
      781 gaggctgcca ccccacatcc tgtgggccac aggcctgaag tctggaggat ctagcggagg
      841 atcctctggc agcgagacac caggaacaag cgagtcagca acaccagaga gcagtggcgg
      901 cagcagcggc ggcagcgaaa tcggcaaggc taccgccaag tacttcttct acagcaacat
      961 catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct
     1021 gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac
     1081 cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac
     1141 aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag
     1201 aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc
     1261 tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga
     1321 gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct
     1381 ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc
     1441 cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa
     1501 gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta
     1561 tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca
     1621 caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct
     1681 ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat
     1741 cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc
     1801 cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt
     1861 gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct
     1921 gtctcagctg ggaggcgacg gcggatcagg aggctctggc ggttcaggtg gatcaggcgg
     1981 tagcggaggt tcaggtggta tggacaagaa gtacagcatc ggcctggcca tcggcaccaa
     2041 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
     2101 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga
     2161 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
     2221 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
     2281 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
     2341 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
     2401 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
     2461 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
     2521 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
     2581 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
     2641 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
     2701 gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
     2761 caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga
     2821 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     2881 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     2941 ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct
     3001 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     3061 gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa
     3121 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     3181 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat
     3241 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa
     3301 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     3361 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     3421 cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg
     3481 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
     3541 gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat
     3601 gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa
     3661 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
     3721 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
     3781 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
     3841 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
     3901 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
     3961 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
     4021 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
     4081 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
     4141 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
     4201 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
     4261 taagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     4321 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca
     4381 gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta
     4441 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc
     4501 cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa
     4561 caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga
     4621 ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca
     4681 gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc
     4741 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat
     4801 cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa
     4861 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa
     4921 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg
     4981 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa
     5041 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag agcggcggga gcggcgggag
     5101 cggggggagc actaatctga gcgacatcat tgagaaggag actgggaaac agctggtcat
     5161 tcaggagtcc atcctgatgc tgcctgagga ggtggaggaa gtgatcggca acaagccaga
     5221 gtctgacatc ctggtgcaca ccgcctacga cgagtccaca gatgagaatg tgatgctgct
     5281 gacctctgac gcccccgagt ataagccttg ggccctggtc atccaggatt ctaacggcga
     5341 gaataagatc aagatgctga gcggaggatc cggaggatct ggaggcagca ccaacctgtc
     5401 tgacatcatc gagaaggaaa caggcaagca gctggtcatc caggagagca tcctgatgct
     5461 gcccgaagaa gtcgaagaag tgatcggaaa caagcctgag agcgatatcc tggtccatac
     5521 cgcctacgac gagagtaccg acgaaaatgt gatgctgctg acatccgacg ccccagagta
     5581 taagccctgg gctctggtca tccaggattc caacggagag aacaaaatca aaatgctgtc
     5641 tggcggctca aaaagaaccg ccgacggcag cgaattcgag cccaagaaga agaggaaagt
     5701 ctaaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     5761 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5821 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5881 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5941 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     6001 agctggggct cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6061 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6121 aagtgtaaag cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6181 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6241 gcggggagag gcggtttgcg tattgggcgc tacagacatg ataagataca ttgatgagtt
     6301 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     6361 tattgcttta tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact
     6421 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     6481 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct
     6541 gctcctcggc cacgaagtgc ttagccctcc cacacataac cagagggcag caattcacga
     6601 atcccaactg ccgtcggctg tccatcactg tccttcacta tggctttgat cccaggatgc
     6661 agatcgagaa gcacctgtcg gcaccgtccg caggggctca agatgcccct gttctcattt
     6721 ccgatcgcga cgatacaagt caggttgcca gctgccgcag cagcagcagt gcccagcacc
     6781 acgagttctg cacaaggtcc cccagtaaaa tgatatacat tgacaccagt gaagatgcgg
     6841 ccgtcgctag agagagctgc gctggcgacg ctgtagtctt cagagatggg gatgctgttg
     6901 attgtagccg ttgctctttc aatgagggtg gattcttctt gagacaaagg cttggccatg
     6961 gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga tgattaattg
     7021 tcaacacgtg ctgatcagat ccgaaaatgg atatacaagc tcccgggagc tttttgcaaa
     7081 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gcagaggcgg
     7141 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     7201 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     7261 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     7321 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     7381 ttccacaccc taactgacac acattccaca gaattcactg actcgctgcg ctcggtcgtt
     7441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7501 ggggataacg caggaaagaa catgtaagtg atctccaaaa aataagtact ttttgactgt
     7561 aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa
     7621 gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt
     7681 aagtacagta atcaagtaaa attactcaag tactttacac ctctgcatgt gagcaaaagg
     7741 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7801 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7861 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7921 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7981 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8041 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8101 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8161 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8221 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8281 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8341 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8401 gtctgacact cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8461 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8521 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8581 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8641 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc
     8701 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8761 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8821 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8881 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8941 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9001 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9061 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9121 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9181 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9241 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9301 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9361 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9421 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9481 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9541 cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta caatctgctc
     9601 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     9661 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     9721 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgggt
     9781 accggcatat ggttcttgac agaggtgtaa aaagtactca aaaattttac tcaagtgaaa
     9841 gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt
     9901 acttgagtaa aagtaaaaaa gtactccatt aaaattgtac ttgagtatta acgcgttgac
     9961 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
    10021 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
    10081 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
    10141 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
    10201 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
    10261 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
    10321 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
    10381 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
    10441 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
    10501 gcggtaggcg tgtacggtgg gaggtctata taagc
//
