LOCUS       sequence-30888-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Sen GL, Reuter JA, Webster DE, Zhu L, Khavari PA
  TITLE     DNMT1 maintains progenitor function in self-renewing somatic tissue.
  JOURNAL   Nature. 2010 Jan 28. 463(7280):563-7.
  PUBMED    20081831
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jan 28. 463(7280):563-7."
COMMENT     Sequence Label: shRNA-DNMT1i(CDS)
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..57
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     42..58
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(127..341)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(187..206)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        352..851
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(387..410)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     784..803
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cgcctactcc ttctctaggc gccggaatta gatcgatctc tcgaggtcga cggtatcgat
       61 aagcttaaaa aggaaccaag caagaagtga tctcttgaat cacttcttgc ttggttccgg
      121 ggatctgtgg tctcatacag aacttataag attcccaaat ccaaagacat ttcacgttta
      181 tggtgatttc ccagaacaca tagcgacatg caaatattgc agggcgccac tcccctgtcc
      241 ctcacagcca tcttcctgcc agggcgcacg cgcgctgggt gttcccgcct agtgacactg
      301 ggcccgcgat tccttggagc gggttgatga cgtcagcgtt cgaattctac cgggtagggg
      361 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
      421 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
      481 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
      541 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
      601 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
      661 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
      721 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
      781 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
      841 cgggcctttc gacctgcagc ccaagctagc ttaccatgac cgagtacaag ccnacggtgc
      901 gcc
//
