LOCUS       sequence-308888-        3567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1841
FEATURES             Location/Qualifiers
     source          1..3567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     302..321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     555..572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(731..758)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(850..936)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     985..1007
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     999..1016
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1000..1016
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1043..1063
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1927..2250
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2271..2290)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2272..2290)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2295..2311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2295..2311)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2413..2432)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2424..3233
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2491..2510)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(3380..3567,1..401)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
       61 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      481 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      541 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
      601 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
      661 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
      721 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
      781 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
      841 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
      901 ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
      961 cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta
     1021 agctcgggcc ccaaataata tgccaaagaa gaagcgtaag gtcctgcaga tggtctccaa
     1081 gggagaggct gtcatcaagg agttcatgcg tttcaaggtc cacatggagg gatccatgaa
     1141 cggacacgag ttcgagatcg agggagaggg agagggacgt ccatacgagg gaacccaaac
     1201 cgccaagctc aaggtcacca agggaggtaa gtttgtgata atccaatttc aattcgcaat
     1261 ggtcatcgtt ttttcaggac cactcccatt ctcctgggac atcctctccc cacaattcat
     1321 gtacggatcc cgtgccttca tcaagcaccc agccgacatc ccagactact acaagcaatc
     1381 cttcccagag ggattcaagt gggagcgtgt gatgaacttc gaggtaagtt tttactccgc
     1441 ttttaacaat ggttgtttga catcattttt tcaggacgga ggagccgtca ccgtcaccca
     1501 agacacctcc ctcgaggacg gaaccctcat ctacaaggtc aagctccgtg gaaccaactt
     1561 cccaccagac ggaccagtca tgcaaaagaa gaccatggga tgggaggcct ccaccgagcg
     1621 tctctaccca gaggacggag tcctcaaagg taagtttgat gaaacggttt cgtcttatat
     1681 acactaatgg tacttttcag gagacatcaa gatggccctc cgtctcaagg acggaggacg
     1741 ttacctcgcc gacttcaaga ccacctacaa ggccaagaag ccagtccaaa tgccaggagc
     1801 ctacaacgtc gaccgtaagc tcgacatcac ctcccacaac gaggactaca ccgtcgtcga
     1861 gcaatacgag cgttccgagg gacgtcactc caccggagga atggacgagc tctacaagta
     1921 agataaatgc aaaatccttt caagcattcc cttcttctct atcactcttc tttctttttg
     1981 tcaaaaaatt ctctcgctaa tttatttgct tttttaatgt tattatttta tgacttttta
     2041 tagtcactga aaagtttgca tctgagtgaa gtgaatgcta tcaaaatgtg attctgtctg
     2101 atgtactttc acaatctctc ttcaattcca ttttgaagtg ctttaaaccc gaaaggttga
     2161 gaaaaatgcg agcgctcaaa tatttgtatt gtgttcgttg agtgacccaa caaaaagagg
     2221 aaactttatt gtgccgccaa gaaaaaagtc tcagccatcc agctgatatc ccctatagtg
     2281 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2341 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     2401 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     2461 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     2521 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     2581 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     2641 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     2701 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     2761 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     2821 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     2881 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     2941 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     3001 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     3061 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     3121 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     3181 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     3241 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct
     3301 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     3361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3541 cgtagttagg ccaccacttc aagaact
//
