LOCUS       sequence-30889-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Sen GL, Reuter JA, Webster DE, Zhu L, Khavari PA
  TITLE     DNMT1 maintains progenitor function in self-renewing somatic tissue.
  JOURNAL   Nature. 2010 Jan 28. 463(7280):563-7.
  PUBMED    20081831
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jan 28. 463(7280):563-7."
COMMENT     Sequence Label: shRNA-DNMT1i(CDS)
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(103..119)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(104..120)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(138..554)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(154..170)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(186..208)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ggatttggga tcttatagtt ctgtatgaga ccacagatcc ccggaaccaa gcaagaagtg
       61 attcaagaga tcacttcttg cttggttcct ttttaagctt atcgataccg tcgacctcga
      121 gagatcgatc taattccggc gcctagagaa ggagtgaggg ctggataaag ggaggatcga
      181 ggcggggtcg aacgaggagg ttcaaggggg agagacgggg cggatggagg aagaggaggc
      241 ggaggcttag ggtgtacaaa gggcttgacc cagggagggg ggtcaaaagc caaggcttcc
      301 caggtcacga tgtaggggac ctggtctggg tgtccatgcg ggccaggtga aaagaccttg
      361 atcttaacct gggtgatgag gtctcggtta aaggtgccgt ctcgcggcca tccgacgtta
      421 aaggttggcc attctgcaga gcagaaggta acccaacgtc tcttcttgac atctaccgac
      481 tggttgtgag cgatccgctc gacatctttc cagtgaccta aggtcaaact taagggagtg
      541 gtaacagtct ggccctaatt ttcagacaaa tacagaaaca cagtcagaca gagacaacac
      601 agaacgatgc tgcagcgctg cagcagacaa gacgcgcggc ttcggttcca aaccgaaagc
      661 aaaaattcag acggaggcgg gaactgtttt aggttctcgt ctcctaccag aaccacatat
      721 cctgacgggg tcggattcca catcgactcc cttcctcagg tcgggccaca aaaacggccc
      781 ccaaagtccc tgggacgtct cccagggttg cggccgggtg ttcagaactc gtcagttcca
      841 ccacgggtcc gccagataca gagctagtta gctaactagt acagacgcag gcgcaaacat
      901 tanatgccgg cacaaacccg c
//
