LOCUS       sequence-30890-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Sen GL, Reuter JA, Webster DE, Zhu L, Khavari PA
  TITLE     DNMT1 maintains progenitor function in self-renewing somatic tissue.
  JOURNAL   Nature. 2010 Jan 28. 463(7280):563-7.
  PUBMED    20081831
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jan 28. 463(7280):563-7."
COMMENT     Sequence Label: shRNA-DNMT1i(3'UTR)
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(113..129)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(114..130)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(148..564)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(164..180)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(196..218)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 agtagtcttn gattgggaat cttataagtt ctgtatgaga ccacagatcc ccgtatgagt
       61 ggaaattaag attcaagaga tcttaatttc cactcatact ttttaagctt atcgataccg
      121 tcgacctcga gagatcgatc taattccggc gcctagagaa ggagtgaggg ctggataaag
      181 ggaggatcga ggcggggtcg aacgaggagg ttcaaggggg agagacgggg cggatggagg
      241 aagaggaggc ggaggcttag ggtgtacaaa gggcttgacc cagggagggg ggtcaaaagc
      301 caaggcttcc caggtcacga tgtaggggac ctggtctggg tgtccatgcg ggccaggtga
      361 aaagaccttg atcttaacct gggtgatgag gtctcggtta aaggtgccgt ctcgcggcca
      421 tccgacgtta aaggttggcc attctgcaga gcagaaggta acccaacgtc tcttcttgac
      481 atctaccgac tggttgtgag cgatccgctc gacatctttc cagtgaccta aggtcaaact
      541 taagggagtg gtaacagtct ggccctaatt ttcagacaaa tacagaaaca cagtcagaca
      601 gagacaacac agaacgatgc tgcagcgctg cagcagacaa gacgcgcggc ttcggttcca
      661 aaccgaaagc aaaaattcag acggaggcgg gaactgtttt aggttctcgt ctcctaccag
      721 aaccacatat cctgacgggg tcggattcca catcgactcc cttcctcagg tcgggccaca
      781 aaaacggccc ccaaagtccc tgggacgtct cccagggttg cggccgggtg ttcagaactc
      841 gtcagttcca ccacgggtcc gccagataca gagctagtta gctaactagt acaga
//
