LOCUS       sequence-308926-        3723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1836
FEATURES             Location/Qualifiers
     source          1..3723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     109..126
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(285..312)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(404..490)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     539..561
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     553..570
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     554..570
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             597..617
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1637..1960
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(1981..2000)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1982..2000)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2005..2021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2005..2021)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2123..2142)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2134..2943
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2201..2220)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3090..3678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3579..3598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
       61 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
      121 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      181 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      241 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
      301 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
      361 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
      421 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
      481 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
      541 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatatgccaa
      601 agaagaagcg taaggtcctg cagatggtct ccaagggaga ggctgtcatc aaggagttca
      661 tgcgtttcaa ggtccacatg gagggatcca tgaacggaca cgagttcgag atcgagggag
      721 agggagaggg acgtccatac gagggaaccc aaaccgccaa gctcaaggtc accaagggag
      781 gtaagtttgt gataatccaa tttcaattcg caatggtcat cgttttttca ggaccactcc
      841 cattctcctg ggacatcctc tccccacaat tcatgtacgg atcccgtgcc ttcatcaagc
      901 acccagccga catcccagac tactacaagc aatccttccc agagggattc aagtgggagc
      961 gtgtgatgaa cttcgaggta agtttttact ccgcttttaa caatggttgt ttgacatcat
     1021 tttttcagga cggaggagcc gtcaccgtca cccaagacac ctccctcgag gacggaaccc
     1081 tcatctacaa ggtcaagctc cgtggaacca acttcccacc agacggacca gtcatgcaaa
     1141 agaagaccat gggatgggag gcctccaccg agcgtctcta cccagaggac ggagtcctca
     1201 aaggtaagtt tgatgaaacg gtttcgtctt atatacacta atggtacttt tcaggagaca
     1261 tcaagatggc cctccgtctc aaggacggag gacgttacct cgccgacttc aagaccacct
     1321 acaaggccaa gaagccagtc caaatgccag gagcctacaa cgtcgaccgt aagctcgaca
     1381 tcacctccca caacgaggac tacaccgtcg tcgagcaata cgagcgttcc gagggacgtc
     1441 actccaccgg aggaatggac gagctctaca agtccggagg atccggagga accggaggat
     1501 cctctctttc tcacggattc ccaccagctg ttgctgctca agatgatgga acccttccaa
     1561 tgtcttgcgc tcaagagtct ggaatggatc gtcacccagc tgcttgcgct tccgctcgta
     1621 tcaacgtcta agataaatgc aaaatccttt caagcattcc cttcttctct atcactcttc
     1681 tttctttttg tcaaaaaatt ctctcgctaa tttatttgct tttttaatgt tattatttta
     1741 tgacttttta tagtcactga aaagtttgca tctgagtgaa gtgaatgcta tcaaaatgtg
     1801 attctgtctg atgtactttc acaatctctc ttcaattcca ttttgaagtg ctttaaaccc
     1861 gaaaggttga gaaaaatgcg agcgctcaaa tatttgtatt gtgttcgttg agtgacccaa
     1921 caaaaagagg aaactttatt gtgccgccaa gaaaaaagtc tcagccatcc agctgatatc
     1981 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     2041 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     2101 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     2161 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     2221 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     2281 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     2341 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     2401 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     2461 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     2521 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     2581 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2641 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2701 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2761 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2821 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2881 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2941 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     3001 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     3061 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3121 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3181 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3241 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3301 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3361 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3421 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3481 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3541 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3601 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3661 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3721 tgg
//
