LOCUS       sequence-30895-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  TITLE     pBNP-NFAT-luciferase
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBNP-NFAT-luciferase
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(102..133)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    234..325
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(266..285)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    339..387
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgcca agctttttac caacagtacc ggaatgccaa gctggaattc gagcttccat
      121 tatataccct ctagagtcta gatcctagca cgcgcaggat ggaaaacaaa aggatagacg
      181 cgtcaggatg gaaaacaaaa ggatagacgc gtaagagctc ggtacctatc gatagagaaa
      241 tgttctggca cctgcacttg cactggggac agcctatttt gctagtttgt tttgtttcgt
      301 tttgttttga tggagagcgt atgttagtac tatcgattca cacaaaaaac caacacacag
      361 atgtaatgaa aataaagata ttttattgcg gccgctccaa gtacctcccg taccttaata
      421 ttacttactt atcatggtag cttgggctgg cgtaatagcg aagaggcccg caccgatcgc
      481 ccttcccaac agttgcgcag cctgaatggc gaatggcaaa ttgtaagcgt taatattttg
      541 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
      601 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
      661 tggaacaa
//
