LOCUS       sequence-308965-        9132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multi-cassette for SapTrap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1820
FEATURES             Location/Qualifiers
     source          1..9132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1700..2023
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(3840..3869)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    3879..3912
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4819..4836)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4819..4835)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4828..4850)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      4899..4985
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5077..5104
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(5263..5280)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5434..6022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5514..5533)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6169..6978)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6892..6911
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6970..6989
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     7091..7107
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7091..7107
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7112..7131
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7112..7130
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(7160..7225)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(7253..7384)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             complement(7406..7426)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    7451..7484
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7837..7861
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7862..8887)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7862..8887)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(8890..8914)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccttggaaaa aatcgaaaaa agaacatttc ccgatgcatt ttcctatttc tcgttgccga
       61 caacgtataa acgttgaata ctcagtcaac gaatttggct gctggcttgg actctcgcac
      121 ggaagagacg cgtgtgtaga tttacgacga aagcaaaaat gatcaccaaa aacggaacgt
      181 tgagctggac ggaaatagtg gtaaagtgac atgattatag tttgaagatt tctaatttca
      241 caattagagc aaatgttgtt cggtatttat tttcaacggt atttatacta ttttccacct
      301 ttttctagaa cattcgagct gcttgttgca aaaggagggc gactcacatt cggtacatgg
      361 aaaagtagtg tacacaataa agagacccag atacattttc cgtctgcgtc tctttgcacc
      421 caccgggagt attttcaaac gaatgcatct aggaccttct agaacattct gtaaggctgc
      481 agaatgcggg tatataagga aagcgggctc agaggaagcc aacacgcttt gttctagtgc
      541 atctaaaaaa cttcgaaaat atgtcaaatc ttctcactgt tcatcaaaat cttccagcac
      601 ttccagttga cgcgacaagt gatgaagttc gcaagaacct catggatatg ttccgtgaca
      661 gacaagcctt ctccgagcac acctggaaga tgcttctctc agtgtgccgt tcttgggctg
      721 cctggtgcaa gcttaacaac cgcaagtggt tcccagccga gccagaagac gttcgtgact
      781 acttgctcta cctccaagct cgtggattgg cggtgaagac gatccaacaa catctcggac
      841 agctcaatat gctccatcgc cgatctggac ttccacgccc atcagattct aatgccgtat
      901 ctctcgtaat gagacgtatc cgcaaggaaa atgttgatgc tggagagaga gccaagcaag
      961 ccctcgcctt cgaaaggtaa gtttaaatat taatgtattc aatcctaaga cttgacgaaa
     1021 ttttcagaac cgacttcgat caggtccgtt ctcttatgga aaattccgat cgttgccagg
     1081 acatccgaaa cctcgccttc ctcggaattg cttacaacac acttctccgt atcgccgaaa
     1141 ttgcccgcat tcgcgtcaag gatattagtc gcacagacgg aggaagaatg ctcatccaca
     1201 tcggacgtac aaaaacgctg gtctcgactg ccggagtcga aaaagcgctc tcccttggag
     1261 ttaccaagct cgttgagcgc tggatttcgg tttccggagt cgccgatgat ccaaacaact
     1321 acctgttctg tcgtgttcgc aagaacggag ttgctgcccc gagtgcaaca tcacaactct
     1381 cgactcgagc tcttgaaggt aagtttaaac aacatattag ctaagaataa gctgaaaata
     1441 aattttcagg aatcttcgaa gctacccacc gtctcatcta cggagccaag gatgattccg
     1501 gacaacgtta cttggcttgg tctggacatt ccgcccgtgt cggagctgcc cgtgacatgg
     1561 ctcgcgctgg agtttccatc ccagagatta tgcaagccgg aggttggacc aacgtcaaca
     1621 ttgtgatgaa ctatattaga aacttggatt ctgaaactgg cgccatggtt cgtcttctcg
     1681 aagacggtga ttaagataaa tgcaaaatcc tttcaagcat tcccttcttc tctatcactc
     1741 ttctttcttt ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt
     1801 ttatgacttt ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat
     1861 gtgattctgt ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa
     1921 cccgaaaggt tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc
     1981 caacaaaaag aggaaacttt attgtgccgc caagaaaaaa gtctcactga atctcaaata
     2041 ttttattaga aaacaccaac aatttagata tttctgtatc cacggttggc gttgacagcc
     2101 tctgatggtg gagacttgtc tgggcactgg cagtactcgg catcctttcc tggtcttccg
     2161 tctggtccag tctctccctg ggttccgtct ggtccttgac gtccttgctt tccagatggt
     2221 ccttctggtc caacggttcc tggctctcct ggtggtccgt gaagaccgtt ctgtccagct
     2281 ggtcctgttg gtccttgatc tcctcttggt ccctttggtc ctggtcttcc gattggatgc
     2341 tcggcgtcgc gtcccttctc tcctggtggt cctggctctc cgatctttcc gatttgaccg
     2401 gatgatcctt gctctcctgg atgtcctggt tgtccatctc ttcctgggtt tccattttgt
     2461 cctcttggtc ctggaagtcc acgtggtcct ggtcttccaa gagctcctgg tggtccaact
     2521 ggtccttgtg ggcaagtgaa gcatccgaca gactcgcgtt gtggagcgat atcatcagct
     2581 ccaactcctg gaacaccgtc aagtcctgga actccgtcga gtcctggaac tcctggaact
     2641 ccctttggtc cggatggtcc agctgggcac ttgttatcag cattgcagtt gcaggttggt
     2701 ccatttccat tggctccatt tggagtcttt ggctggttga agactggtgg aactgctggt
     2761 ggttgacctg gtggagcaga tggggatcca tcagcatgtg gagttggggt agcgttggtc
     2821 tcttcatatt gacaacggac acgcttgcta gatcttccga tgacaactat gtccttccac
     2881 aatccattgg tagattgcta gaaaattaaa aattatttaa aattcaaaat ataatttaac
     2941 tcactctgaa cacattcatc tcggtatcaa gcttctctct gaatccatca acctccgagt
     3001 agatggttga catggtcatg aagcatacca taagagtggc gacagtgacc gaagccgtca
     3061 cataacacgc aagttttaca gacatctctg aaattatcaa atttaggatt tcaaaataat
     3121 tcggccaaag aaatgtaact cacctagtct tcgagaagca gagacttgtc gatttcctac
     3181 cggattcgtc tccttttatt aggaaatctt tccgcaagtt ttcaagtttt ttttgttttt
     3241 tttttacatt accgacgaga cagtgtgcga gacgcagaga cgataaaaga ctggataaga
     3301 acgggagata aggagtacgg atgtaggtga ggaatgtggc cgaaaggaca gatgggcttt
     3361 attcgggaag attggttggg aaatgaacca gagaagaatg actgaataaa gttgttgaaa
     3421 cttggtgaga atggaagata gacaatcgag ggcagctagt ttccttcctc ctctatatat
     3481 ttttatgttt tcttttaaac cttcctccat atttctgaat tgaatgttgt tgcatattgt
     3541 gtgtccttat ttcatttcca tcatcctttt gtgtgtttcc cccttccacc aattcccccc
     3601 acgtcctaat gaaatgaaag cgagaagata aataaacttg cacattgtgt tcagatgaga
     3661 ggagcggaat acgaattggg agacggaaag agaaaaaaaa atgtttagat ttggattgaa
     3721 ttaatttttt agaaagcaag aaaaaaaaca caaagagaat aaataaataa taggaaaaaa
     3781 aacaaatatt cagtatttaa aatttgaaaa ttcaacgacg ttggcgtcga tcatccttag
     3841 aggtcctcct cggagataag cttttgctct ccctgaaaat aacttcgtat aatgtatgct
     3901 atacgaagtt attttaaact taccttgtag agctcgtcca ttccgtgggt gattccggcg
     3961 gcggtgacga actcgaggag gaccatgtgg tcacgcttct cgtttgggtc cttggagagg
     4021 gcggattggg tggagaggta gtggttgtct gggaggagga ctggtccgtc tccgattggg
     4081 gtgttttgtt ggtagtggtc ggcgagttgg acggatccgt cctcgatgtt gtgacggatc
     4141 tgaaaattta aatcagatta gttagttagt aaaatcatgt ttaaacttac cttgaagttg
     4201 accttgattc cgttcttttg cttgtcggcc atgatgtaga cgttgtggga gttgtagttg
     4261 tactcgagct tgtgtccgag gatgtttccg tcctccttga agtcgattcc ctgaaaattt
     4321 aaatatgtat ggttagttag taccgaactg tttaaactta ccttgagctc gatacggttg
     4381 acgagggtgt ctccctcgaa cttgacctcg gcacgggtct tgtagtttcc gtcgtcctga
     4441 aaatttaaat aatcagtagt tagtatatat atgtttaaac ttaccttgaa gaagatggta
     4501 cgctcttgga cgtatccctc tggcatggcg gacttgaaga agtcgtgacg cttcatgtgg
     4561 tctgggtaac gggagaagca ttggactccg tagcagaagg tggtgacgag ggttggccat
     4621 gggactggga gctttccggt ggtgcagatg aacttgaggg tgagctttcc gtaggtggcg
     4681 tctccctctc cctctccgga gacggagaac ttgtgtccgt tgacgtctcc gtcgagctcg
     4741 acgaggattg ggacaactcc agtgaacaat tcttctcctt tactcatcga agagcattat
     4801 ttggggcccg agcttaagac tggccgtcgt tttacaacgt cgtgactggg aaaacatcca
     4861 tgctagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     4921 cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     4981 caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag
     5041 gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg acggatggcc
     5101 tttttgcgtt tctacaaact cttcctggct agcggtacgc gtattaattg cgttgcgctc
     5161 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5221 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5281 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5341 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5401 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5461 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5521 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5581 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5641 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5701 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5761 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5821 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5881 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5941 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6001 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6061 gtggaacgac gcgtaactca cgttaaggga ttttggtcat gagcttgcgc cgtcccgtca
     6121 agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc
     6181 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     6241 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     6301 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     6361 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     6421 gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     6481 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     6541 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     6601 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     6661 ctggaatgct gtttttccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     6721 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     6781 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     6841 atcgggcttc ccatacaagc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     6901 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tcgacgtttc
     6961 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
     7021 tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacggg
     7081 ccagagctgc caggaaacag ctatgaccat gtaatacgac tcactatagg ggatatcagc
     7141 tggatggcgc tcttcgcgtc ttgtcatcgt catccttgta atcgatatca tgatctttat
     7201 aatcaccgtc atggtctttg tagtcagatc ctgagccaga accgccacca cccttcacga
     7261 acgccgccgc ctccgggcca ccgcttgatt tttggcagga aaccatcacg ttcttccggt
     7321 atgatctcac cggtggccat cccacaactt gtgccttggc cggaggtttg gctggatctt
     7381 taggcatccc gccacctccg gatccggatt gaaagtacag attctctcct ccgctgccgc
     7441 caccctgaaa ataacttcgt ataatgtatg ctatacgaag ttattaaaaa ttgcggtcat
     7501 aaactgaaac gtaacatatg ataaggtatt ttgtgtgcgg ttttttctat gatgtttcaa
     7561 aaaaatttat cagaagtaaa aaacataaga agtgtacaca gaacattgtg tagattttgg
     7621 gatagaatta ttattaatgg ggggagggag cacaattttt ttcgactttt tattacgaat
     7681 taattctatt tttgaatcta cacaatttca ttgttagagg tgacttaaaa gaagctaaaa
     7741 aacaaagaaa ttaagagaag ttttttgata ataacaaaaa tagggggtgg gagcacaggg
     7801 agaaagagca tgtagggatg ttgaagagta attggaccca ctttgtacaa gaaagctggg
     7861 tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt
     7921 ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag
     7981 tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga
     8041 aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc
     8101 ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct
     8161 ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc
     8221 cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat
     8281 tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa
     8341 gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt
     8401 gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt
     8461 gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag
     8521 cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca
     8581 ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga
     8641 attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa
     8701 cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc
     8761 ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg
     8821 agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct
     8881 ttttcatgga gcctgctttt ttgtacaaac ttgcggcacc gcctgacata ttaccttaaa
     8941 attcaaaaat taatttcaga tcagtcataa ccaagaaaac aaaataaacg ttaggacaga
     9001 aaattcaaat taattaaaaa ttatgctgaa taagaaaatt acatgcaaag ccttattttc
     9061 ctcatagaaa cagttgaata acatattttt taattgaaaa catagcaaaa ttcgtggtaa
     9121 aatttgtcaa aa
//
