LOCUS       sequence-308973-       13254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  activating MTOR mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan
            A, Beck AH, Sabatini DM
  TITLE     A diverse array of cancer-associated MTOR mutations are 
            hyperactivating and can predict rapamycin sensitivity.
  JOURNAL   Cancer Discov. 2014 May;4(5):554-63. doi: 
            10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14.
  PUBMED    24631838
REFERENCE   2  (bases 1 to 13254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0929"; journalName: "Cancer Discov"; date: 
            "2014-05"; volume: "4"; issue: "5"; pages: "554-63"
COMMENT     Sequence Label: pcDNA3-FLAG-MTOR-I2500F
FEATURES             Location/Qualifiers
     source          1..13254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        136..515
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        516..719
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     670..690
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     764..783
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        764..782
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      868..877
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3749..3758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6958..7236
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             6970..7242
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
     primer_bind     complement(8657..8673)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8657..8673)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8708..8726)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8709..8726)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8746..8763)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8752..8976
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9022..9450
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9109..9128)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9319..9340
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9459..9479)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9464..9793
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9644..9779
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9706..9725
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9860..10654
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9914..9933)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10524..10543
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10828..10949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10865..10884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10919..10938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10998..11014)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10998..11014)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11011..11033)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11022..11038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11046..11052,11053..11070,11071..11076))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11091..11112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11229..11246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11400..11988)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11480..11499)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12159..12950,12951..13019))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12782..12801
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13020..13124)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13199..13218)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
       61 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
      121 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
      181 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      241 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      301 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      361 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      421 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      481 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      541 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      601 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      661 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      721 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      781 gggagaccca agcttggtac cgagctcgga tccactagta acggccgcca gtgtgctgga
      841 attctgcaga tatccatcac actggcggcc gccatggatt ataaagatga tgatgatatg
      901 cttggaaccg gacctgccgc cgccaccacc gctgccacca catctagcaa tgtgagcgtc
      961 ctgcagcagt ttgccagtgg cctaaagagc cggaatgagg aaaccagggc caaagccgcc
     1021 aaggagctcc agcactatgt caccatggaa ctccgagaga tgagtcaaga ggagtctact
     1081 cgcttctatg accaactgaa ccatcacatt tttgaattgg tttccagctc agatgccaat
     1141 gagaggaaag gtggcatctt ggccatagct agcctcatag gagtggaagg tgggaatgcc
     1201 acccgaattg gcagatttgc caactatctt cggaacctcc tcccctccaa tgacccagtt
     1261 gtcatggaaa tggcatccaa ggccattggc cgtcttgcca tggcagggga cacttttacc
     1321 gctgagtacg tggaatttga ggtgaagcga gccctggaat ggctgggtgc tgaccgcaat
     1381 gagggccgga gacatgcagc tgtcctggtt ctccgtgagc tggccatcag cgtccctacc
     1441 ttcttcttcc agcaagtgca acccttcttt gacaacattt ttgtggccgt gtgggacccc
     1501 aaacaggcca tccgtgaggg agctgtagcc gcccttcgtg cctgtctgat tctcacaacc
     1561 cagcgtgagc cgaaggagat gcagaagcct cagtggtaca ggcacacatt tgaagaagca
     1621 gagaagggat ttgatgagac cttggccaaa gagaagggca tgaatcggga tgatcggatc
     1681 catggagcct tgttgatcct taacgagctg gtccgaatca gcagcatgga gggagagcgt
     1741 ctgagagaag aaatggaaga aatcacacag cagcagctgg tacacgacaa gtactgcaaa
     1801 gatctcatgg gcttcggaac aaaacctcgt cacattaccc ccttcaccag tttccaggct
     1861 gtacagcccc agcagtcaaa tgccttggtg gggctgctgg ggtacagctc tcaccaaggc
     1921 ctcatgggat ttgggacctc ccccagtcca gctaagtcca ccctggtgga gagccggtgt
     1981 tgcagagact tgatggagga gaaatttgat caggtgtgcc agtgggtgct gaaatgcagg
     2041 aatagcaaga actcgctgat ccaaatgaca atccttaatt tgttgccccg cttggctgca
     2101 ttccgacctt ctgccttcac agatacccag tatctccaag ataccatgaa ccatgtccta
     2161 agctgtgtca agaaggagaa ggaacgtaca gcggccttcc aagccctggg gctactttct
     2221 gtggctgtga ggtctgagtt taaggtctat ttgcctcgcg tgctggacat catccgagcg
     2281 gccctgcccc caaaggactt cgcccataag aggcagaagg caatgcaggt ggacgccaca
     2341 gtcttcactt gcatcagcat gctggctcga gcaatggggc caggcatcca gcaggatatc
     2401 aaggagctgc tggagcccat gctggcagtg ggactaagcc ctgccctcac tgcagtgctc
     2461 tacgacctga gccgtcagat tccacagcta aagaaggaca ttcaagatgg gctactgaaa
     2521 atgctgtccc tggtccttat gcacaaaccc cttcgccacc caggcatgcc caagggcctg
     2581 gcccatcagc tggcctctcc tggcctcacg accctccctg aggccagcga tgtgggcagc
     2641 atcactcttg ccctccgaac gcttggcagc tttgaatttg aaggccactc tctgacccaa
     2701 tttgttcgcc actgtgcgga tcatttcctg aacagtgagc acaaggagat ccgcatggag
     2761 gctgcccgca cctgctcccg cctgctcaca ccctccatcc acctcatcag tggccatgct
     2821 catgtggtta gccagaccgc agtgcaagtg gtggcagatg tgcttagcaa actgctcgta
     2881 gttgggataa cagatcctga ccctgacatt cgctactgtg tcttggcgtc cctggacgag
     2941 cgctttgatg cacacctggc ccaggcggag aacttgcagg ccttgtttgt ggctctgaat
     3001 gaccaggtgt ttgagatccg ggagctggcc atctgcactg tgggccgact cagtagcatg
     3061 aaccctgcct ttgtcatgcc tttcctgcgc aagatgctca tccagatttt gacagagttg
     3121 gagcacagtg ggattggaag aatcaaagag cagagtgccc gcatgctggg gcacctggtc
     3181 tccaatgccc cccgactcat ccgcccctac atggagccta ttctgaaggc attaattttg
     3241 aaactgaaag atccagaccc tgatccaaac ccaggtgtga tcaataatgt cctggcaaca
     3301 ataggagaat tggcacaggt tagtggcctg gaaatgagga aatgggttga tgaacttttt
     3361 attatcatca tggacatgct ccaggattcc tctttgttgg ccaaaaggca ggtggctctg
     3421 tggaccctgg gacagttggt ggccagcact ggctatgtag tagagcccta caggaagtac
     3481 cctactttgc ttgaggtgct actgaatttt ctgaagactg agcagaacca gggtacacgc
     3541 agagaggcca tccgtgtgtt agggctttta ggggctttgg atccttacaa gcacaaagtg
     3601 aacattggca tgatagacca gtcccgggat gcctctgctg tcagcctgtc agaatccaag
     3661 tcaagtcagg attcctctga ctatagcact agtgaaatgc tggtcaacat gggaaacttg
     3721 cctctggatg agttctaccc agctgtgtcc atggtggccc tgatgcggat cttccgagac
     3781 cagtcactct ctcatcatca caccatggtt gtccaggcca tcaccttcat cttcaagtcc
     3841 ctgggactca aatgtgtgca gttcctgccc caggtcatgc ccacgttcct taatgtcatt
     3901 cgagtctgtg atggggccat ccgggaattt ttgttccagc agctgggaat gttggtgtcc
     3961 tttgtgaaga gccacatcag accttatatg gatgaaatag tcaccctcat gagagaattc
     4021 tgggtcatga acacctcaat tcagagcacg atcattcttc tcattgagca aattgtggta
     4081 gctcttgggg gtgaatttaa gctctacctg ccccagctga tcccacacat gctgcgtgtc
     4141 ttcatgcatg acaacagccc aggccgcatt gtctctatca agttactggc tgcaatccag
     4201 ctgtttggcg ccaacctgga tgactacctg catttactgc tgcctcctat tgttaagttg
     4261 tttgatgccc ctgaagctcc actgccatct cgaaaggcag cgctagagac tgtggaccgc
     4321 ctgacggagt ccctggattt cactgactat gcctcccgga tcattcaccc tattgttcga
     4381 acactggacc agagcccaga actgcgctcc acagccatgg acacgctgtc ttcacttgtt
     4441 tttcagctgg ggaagaagta ccaaattttc attccaatgg tgaataaagt tctggtgcga
     4501 caccgaatca atcatcagcg ctatgatgtg ctcatctgca gaattgtcaa gggatacaca
     4561 cttgctgatg aagaggagga tcctttgatt taccagcatc ggatgcttag gagtggccaa
     4621 ggggatgcat tggctagtgg accagtggaa acaggaccca tgaagaaact gcacgtcagc
     4681 accatcaacc tccaaaaggc ctggggcgct gccaggaggg tctccaaaga tgactggctg
     4741 gaatggctga gacggctgag cctggagctg ctgaaggact catcatcgcc ctccctgcgc
     4801 tcctgctggg ccctggcaca ggcctacaac ccgatggcca gggatctctt caatgctgca
     4861 tttgtgtcct gctggtctga actgaatgaa gatcaacagg atgagctcat cagaagcatc
     4921 gagttggccc tcacctcaca agacatcgct gaagtcacac agaccctctt aaacttggct
     4981 gaattcatgg aacacagtga caagggcccc ctgccactga gagatgacaa tggcattgtt
     5041 ctgctgggtg agagagctgc caagtgccga gcatatgcca aagcactaca ctacaaagaa
     5101 ctggagttcc agaaaggccc cacccctgcc attctagaat ctctcatcag cattaataat
     5161 aagctacagc agccggaggc agcggccgga gtgttagaat atgccatgaa acactttgga
     5221 gagctggaga tccaggctac ctggtatgag aaactgcacg agtgggagga tgcccttgtg
     5281 gcctatgaca agaaaatgga caccaacaag gacgacccag agctgatgct gggccgcatg
     5341 cgctgcctcg aggccttggg ggaatggggt caactccacc agcagtgctg tgaaaagtgg
     5401 accctggtta atgatgagac ccaagccaag atggcccgga tggctgctgc agctgcatgg
     5461 ggtttaggtc agtgggacag catggaagaa tacacctgta tgatccctcg ggacacccat
     5521 gatggggcat tttatagagc tgtgctggca ctgcatcagg acctcttctc cttggcacaa
     5581 cagtgcattg acaaggccag ggacctgctg gatgctgaat taactgcaat ggcaggagag
     5641 agttacagtc gggcatatgg ggccatggtt tcttgccaca tgctgtccga gctggaggag
     5701 gttatccagt acaaacttgt ccccgagcga cgagagatca tccgccagat ctggtgggag
     5761 agactgcagg gctgccagcg tatcgtagag gactggcaga aaatccttat ggtgcggtcc
     5821 cttgtggtca gccctcatga agacatgaga acctggctca agtatgcaag cctgtgcggc
     5881 aagagtggca ggctggctct tgctcataaa actttagtgt tgctcctggg agttgatccg
     5941 tctcggcaac ttgaccatcc tctgccaaca gttcaccctc aggtgaccta tgcctacatg
     6001 aaaaacatgt ggaagagtgc ccgcaagatc gatgccttcc agcacatgca gcattttgtc
     6061 cagaccatgc agcaacaggc ccagcatgcc atcgctactg aggaccagca gcataagcag
     6121 gaactgcaca agctcatggc ccgatgcttc ctgaaacttg gagagtggca gctgaatcta
     6181 cagggcatca atgagagcac aatccccaaa gtgctgcagt actacagcgc cgccacagag
     6241 cacgaccgca gctggtacaa ggcctggcat gcgtgggcag tgatgaactt cgaagctgtg
     6301 ctacactaca aacatcagaa ccaagcccgc gatgagaaga agaaactgcg tcatgccagc
     6361 ggggccaaca tcaccaacgc caccactgcc gccaccacgg ccgccactgc caccaccact
     6421 gccagcaccg agggcagcaa cagtgagagc gaggccgaga gcaccgagaa cagccccacc
     6481 ccatcgccgc tgcagaagaa ggtcactgag gatctgtcca aaaccctcct gatgtacacg
     6541 gtgcctgccg tccagggctt cttccgttcc atctccttgt cacgaggcaa caacctccag
     6601 gatacactca gagttctcac cttatggttt gattatggtc actggccaga tgtcaatgag
     6661 gccttagtgg agggggtgaa agccatccag attgatacct ggctacaggt tatacctcag
     6721 ctcattgcaa gaattgatac gcccagaccc ttggtgggac gtctcattca ccagcttctc
     6781 acagacattg gtcggtacca cccccaggcc ctcatctacc cactgacagt ggcttctaag
     6841 tctaccacga cagcccggca caatgcagcc aacaagattc tgaagaacat gtgtgagcac
     6901 agcaacaccc tggtccagca ggccatgatg gtgagcgagg agctgatccg agtggccatc
     6961 ctctggcatg agatgtggca tgaaggcctg gaagaggcat ctcgtttgta ctttggggaa
     7021 aggaacgtga aaggcatgtt tgaggtgctg gagcccttgc atgctatgat ggaacggggc
     7081 ccccagactc tgaaggaaac atcctttaat caggcctatg gtcgagattt aatggaggcc
     7141 caagagtggt gcaggaagta catgaaatca gggaatgtca aggacctcac ccaagcctgg
     7201 gacctctatt atcatgtgtt ccgacgaatc tcaaagcagc tgcctcagct cacatcctta
     7261 gagctgcaat atgtttcccc aaaacttctg atgtgccggg accttgaatt ggctgtgcca
     7321 ggaacatatg accccaacca gccaatcatt cgcattcagt ccatagcacc gtctttgcaa
     7381 gtcatcacat ccaagcagag gccccggaaa ttgacactta tgggcagcaa cggacatgag
     7441 tttgttttcc ttctaaaagg ccatgaagat ctgcgccagg atgagcgtgt gatgcagctc
     7501 ttcggcctgg ttaacaccct tctggccaat gacccaacat ctcttcggaa aaacctcagc
     7561 atccagagat acgctgtcat ccctttatcg accaactcgg gcctcattgg ctgggttccc
     7621 cactgtgaca cactgcacgc cctcatccgg gactacaggg agaagaagaa gatccttctc
     7681 aacatcgagc atcgcatcat gttgcggatg gctccggact atgaccactt gactctgatg
     7741 cagaaggtgg aggtgtttga gcatgccgtc aataatacag ctggggacga cctggccaag
     7801 ctgctgtggc tgaaaagccc cagctccgag gtgtggtttg accgaagaac caattatacc
     7861 cgttctttag cggtcatgtc aatggttggg tatattttag gcctgggaga tagacaccca
     7921 tccaacctga tgctggaccg tctgagtggg aagatcctgc acattgactt tggggactgc
     7981 tttgaggttg ctatgacccg agagaagttt ccagagaaga ttccatttag actaacaaga
     8041 atgttgacca atgctatgga ggttacaggc ctggatggca actacagaat cacatgccac
     8101 acagtgatgg aggtgctgcg agagcacaag gacagtgtca tggccgtgct ggaagccttt
     8161 gtctatgacc ccttgctgaa ctggaggctg atggacacaa ataccaaagg caacaagcga
     8221 tcccgaacga ggacggattc ctactctgct ggccagtcag tcgaaatttt ggacggtgtg
     8281 gaacttggag agccagccca taagaaaacg gggaccacag tgccagaatc tattcattct
     8341 ttcattggag acggtttggt gaaaccagag gccctaaata agaaagctat ccagtttatt
     8401 aacagggttc gagataagct cactggtcgg gacttctctc atgatgacac tttggatgtt
     8461 ccaacgcaag ttgagctgct catcaaacaa gcgacatccc atgaaaacct ctgccagtgc
     8521 tatattggct ggtgcccttt ctggtaactg gaggcccaga tgtgcccatc acgttttttc
     8581 tgaggctttt gtactttagt aaatgcttcc actaaactga aaccatggtg agaaagtttg
     8641 actttgttaa atgggggatc cactagttct agagcggccg ctcgagcatg catctagagg
     8701 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
     8761 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     8821 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     8881 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     8941 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     9001 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     9061 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     9121 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     9181 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     9241 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9301 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9361 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9421 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     9481 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9541 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9601 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9661 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9721 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9781 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     9841 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     9901 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     9961 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
    10021 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
    10081 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
    10141 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
    10201 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
    10261 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
    10321 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
    10381 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
    10441 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
    10501 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
    10561 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
    10621 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
    10681 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
    10741 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
    10801 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
    10861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    10921 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
    10981 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    11041 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    11101 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    11161 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    11221 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    11281 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    11341 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    11401 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    11461 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    11521 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    11581 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    11641 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    11701 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    11761 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    11821 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    11881 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    11941 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    12001 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    12061 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    12121 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    12181 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    12241 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    12301 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    12361 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    12421 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    12481 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    12541 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    12601 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    12661 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    12721 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    12781 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    12841 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    12901 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    12961 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    13021 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    13081 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    13141 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
    13201 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgt
//
