LOCUS       sequence-30899-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Carroll P, Muttucumaru DG, Parish T
  TITLE     Use of a tetracycline-inducible system for conditional expression in
            Mycobacterium tuberculosis and Mycobacterium smegmatis.
  JOURNAL   Appl Environ Microbiol. 2005 Jun . 71(6):3077-84.
  PUBMED    15933004
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2005 Jun . 71(6):3077-84."
COMMENT     Sequence Label: pTACT1
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(204..250)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    342..360
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    427..445
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(543..562)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 atcgcggcct cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
       61 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
      121 aacatcagag attttgagac acaacgtggc tttccccccc ccccgacgtc aggtggctag
      181 ctgatcaccg cggccatgat ggcataaaac gaaaggccca gtctttcgac tgagcctttc
      241 gttttatttg atgcctggca gtcgatcgta cgctagttaa ctacgtcgac atcgataagc
      301 ttcgaattcg agctcggtac catcaagctt attttaatta tactctatca atgatagagt
      361 gtcaatattt tttttagttt ttcatgaact cgaggggatc caaataaaaa actagtttga
      421 caaataactc tatcaatgat agagtgtcaa caaaaaggag gaattaatga tgtctagatt
      481 agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg
      541 tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca
      601 tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca
      661 tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa
      721 aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg
      781 gcctac
//
