LOCUS       sequence-308996-        8944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  New color variant of Fucci (Fluorescent ubiquitination-based cell 
            cycle indicator) : Fucci(CA)5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Ando R, Sakaue-Sawano A, Shoda K, Miyawaki A
  TITLE     Two coral fluorescent proteins of distinct colors for sharp 
            visualization of cell-cycle progression.
  JOURNAL   Cell Struct Funct. 2023 Jun 30. doi: 10.1247/csf.23028.
  PUBMED    37394513
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Struct
            Funct. 2023 Jun 30. doi: 10.1247/csf.23028."
COMMENT     Sequence Label: tFucci(CA)5
FEATURES             Location/Qualifiers
     source          1..8944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   672..706
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1009..1388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1390..1669
                     /label=chicken beta-actin promoter
     intron          1670..2686
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2610..2632
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2694..2713
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2753..2762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3806..3862
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4604..4933
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    5006..5555
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5150..5167)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5377..5396
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6026..6043)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6032..6256
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6303..6319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6327..6343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6351..6357,6358..6375,6376..6381))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6396..6417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6651..6713
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6754..6774)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6754..6772)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6793..6809)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6817..6833
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6841..6847,6848..6865,6866..6871))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6886..6907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7024..7041)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7195..7783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7275..7294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7954..8745,8746..8814))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8577..8596
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8815..8919)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg gggcgcgcca
      661 ttctagatta accctagaaa gatagtctgc gtaaaattga cgcatgcatt cttgaaatat
      721 tgctctctct ttctaaatag cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc
      781 gcttggagct cccgtgaggc gtgcttgtca atgcggtaag tgtcactgat tttgaactat
      841 aacgaccgcg tgagtcaaaa tgacgcatga ttatctttta cgtgactttt aagatttaac
      901 tcatacgata attatattgt tatttcatgt tctacttacg tgataactta ttatatatat
      961 attttcttgt tatagatatc aactagaatg ctagcatggg cccatctcga cattgattat
     1021 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     1081 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     1141 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     1201 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     1261 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     1321 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     1381 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     1441 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     1501 gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag
     1561 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
     1621 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
     1681 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
     1741 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
     1801 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
     1861 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
     1921 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
     1981 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
     2041 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     2101 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
     2161 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
     2221 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
     2281 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
     2341 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     2401 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     2461 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     2521 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     2581 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     2641 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     2701 gctggttatt gtgctgtctc atcattttgg caaagaattg gtcgacggat ccgccaccat
     2761 ggagaacgtc aggagaaaga caggcattca gacggaaatg aaaacgaagc tccacatgga
     2821 tggtatggtg aatggacaca gctttgagat caaaggggag gggaagggaa gcccgtacga
     2881 gggggtgcag acaatgaagc tgaaggttac taagggcgcc ccacttccct tctctataga
     2941 cattcttttg cctcagtgca tgtatggcag caaacctttc attaaatatc ctgagaacat
     3001 ccccgattat ttgaaactct catttccaga gggtatcact tgggagagga caatgacatt
     3061 cgaggacggc gcagtatgcg atgtatctaa tgattccaga ctggtcggca attgctttat
     3121 ctacactgtg aagtttcagg gagtcaattt tccacttgat ggccccgtta tgcaaaagaa
     3181 gacccgaggc tgggaaccct ccactgaagt gctgtacgaa tgtgatgggt ggatgcgcgg
     3241 gttggtggac atagctctga aactggaaaa cggtggacat tatatgtgta attttaagac
     3301 cacctacaaa tccaagaaag gtcttgaggt gccaccttac catttcgtgg atcataaact
     3361 cgacctgctc agtcataaca ccgacggagc cacctttgag gagttcgaac aaggtgaaat
     3421 cgctcacgcg cacttgagta aactggccgg atatccatca cactggcggc cgctcgagat
     3481 ggagcagcgc cgcgtcaccg acttcttcgc gcgccgccgc cccgggcccc cccgcatcgc
     3541 gccgcccaag ctggcctgcc gcacccccag ccccgccagg cccgcactcc gcgccccggc
     3601 ctccgctacc agtggcagcc gcaagcgcgc ccgcccgccc gccgcccccg gacgcgacca
     3661 ggccaggcca ccggcccgcg cggccgcgcg gctgtcggtg gacgaggttt ccagccccag
     3721 tacccccgag gccccagaca tcccagcctg cccttctccg ggccagaaga taaagaaaac
     3781 cggtcgcgcc aagcgcggca gcggcgccac caacttcagc ctgctgaagc aggccggcga
     3841 cgtggaggag aaccccggcc cccgcgaatt catgagtaaa ctccagaaag gtgtggagaa
     3901 agagatgaag attaagctca ctatgaactg caccgtgaac gaacggaact ttgtgatcac
     3961 agggcagggg gctggggaac cttatgatgg aactcagaca ctttacctga cagtggaggg
     4021 cggaaagaca ctgaacttca gcttcgatgt actgacccca gcctttcagt acggcaatag
     4081 ggcgtttacc aagtaccccg gtaacatacc agacttcttc aaacagacct tcagtggcgg
     4141 gggatacaca tggaaaagaa caatgaccta tgaagacggc ggcgtctcaa ccgttgagtc
     4201 cgatatcagc gttcaagggg actgcttcca ttataaaata caattcaacg gaaagttccc
     4261 cccccacggc ccagtcatgc aaaaggaaac agtcaaatgg gaaccttcca ccgaggtgat
     4321 gtacaaggac gataaaaacg atggagtgct gaagggcgac gtgaatatgg ccctgctgtt
     4381 gaaggatggt ggtcacctca gagtcgattt caacacttct tatattccca aaaataaggt
     4441 ggagaagatg ccagattacc acttcgtgga ccaccggatc gagatcctgg agaagcccga
     4501 aggacgccct gtcaagctct acgcaggggc tgtggccagg tacagcctgc tgcctgaaaa
     4561 gaacctgaat aagggatatc catcacactg gcggccgctc gagatgaatc ccagtatgaa
     4621 gcagaaacaa gaagaaatca aagagaatat aaagaatagt tctgtcccaa gaagaactct
     4681 gaagatgatt cagccttctg catctggatc tcttgttgga agagaaaatg agctgtccgc
     4741 aggcttgtcc aaaaggaaac atcggaatga ccacttaaca tctacaactt ccagccctgg
     4801 ggttattgtc ccagaatcta gtgaaaataa aaatcttgga ggagtcaccc aggagtcatt
     4861 tgatcttatg attaaagaaa atccatcctc tcagtattgg aaggaagtgg cagaaaaacg
     4921 gagaaaggcg ctgtaatcta gagttaacta acttaagcta gcaacggttt ccctctagcg
     4981 ggatcaattc cgcccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5041 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     5101 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5161 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5221 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5281 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5341 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5401 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5461 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5521 ggggacgtgg ttttcctttg aaaaacacga taataccatg gtcatgaaaa catttaacat
     5581 ttctcaacaa gatctagaat tagtagaagt agcgacagag aagattacaa tgctttatga
     5641 ggataataaa catcatgtgg gagcggcaat tcgtacgaaa acaggagaaa tcatttcggc
     5701 agtacatatt gaagcgtata taggacgagt aactgtttgt gcagaagcca ttgcgattgg
     5761 tagtgcagtt tcgaatggac aaaaggattt tgacacgatt gtagctgtta gacaccctta
     5821 ttctgacgaa gtagatagaa gtattcgagt ggtaagtcct tgtggtatgt gtagggagtt
     5881 gatttcagac tatgcaccag attgttttgt gttaatagaa atgaatggca agttagtcaa
     5941 aactacgatt gaagaactca ttccactcaa atatacccga aattaaaagt cgagatgcat
     6001 cgatgatcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6061 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6121 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     6181 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     6241 gcggtgggct ctatggcttc tgaggcggaa agaacctgca gcccaagctt ggcgtaatca
     6301 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6361 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6421 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatcttaaaa
     6481 gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac
     6541 ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta
     6601 atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa
     6661 ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaatct
     6721 agtatacgcg tttaattaat ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     6781 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6841 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6901 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6961 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7021 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7081 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7141 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7201 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7261 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7321 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7381 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7441 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7501 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7561 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7621 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7681 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7741 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7801 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7861 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7921 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7981 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8041 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8101 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8161 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8221 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8281 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8341 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8401 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8461 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8521 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8581 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8641 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8701 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8761 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8821 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8881 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8941 acct
//
