LOCUS       sequence-30900-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Carroll P, Muttucumaru DG, Parish T
  TITLE     Use of a tetracycline-inducible system for conditional expression in
            Mycobacterium tuberculosis and Mycobacterium smegmatis.
  JOURNAL   Appl Environ Microbiol. 2005 Jun . 71(6):3077-84.
  PUBMED    15933004
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2005 Jun . 71(6):3077-84."
COMMENT     Sequence Label: pHLEGM2
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    101..119
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             226..852
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(302..321)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ggaagtcttc tcctttctca tatgtatatc tccttcttaa aggatccccg ggtaccgagc
       61 tcgaattcga gctcggtacc atcaagctta ttttaattat actctatcaa tgatagagtg
      121 tcaatatttt ttttagtttt tcatgaactc gaggggatcc aaataaaaaa ctagtttgac
      181 aaataactct atcaataata gagtgtcaac aaaaaggagg aattaatgat gtctagatta
      241 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt
      301 ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat
      361 gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat
      421 actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa
      481 agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg
      541 cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt
      601 ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc
      661 gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat
      721 agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca
      781 gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa
      841 agtgggtctt aaagcagcat aacc
//
