LOCUS       sequence-309002-        2726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human TFAM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2726)
  TITLE     Allen Institute for Cell Sciences Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-72:TFAM-mEGFP
FEATURES             Location/Qualifiers
     source          1..2726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1013..1015,1016..1729)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1055..1076)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1316..1335)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1663..1684
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acctaaagct acagggaagg aataaaaaca gactttgaag ttagtttatg acaacacata
       61 gattatacct gaatattaat atttccgaat caaaaatatg taccttcatt cagttcattt
      121 ttgtaaattg taacagtttg atagtttttg tttagagaga aaagatgtta agaattgatg
      181 aggctttgcc tgtaattctt ggtatcttga gtttttgttg gaatgaataa taaaggtcac
      241 tgaagtcccc atatctacct taacttaaac atatattgtt ttacaggaaa agctgaagac
      301 tgtaaaggaa aactggaaaa atctgtctga ctctgaaaag gaagtgagta ttacggttgt
      361 tagtctcaag tgtctactta ggatatctgt gagagaatgt attagggcaa ggcttgattc
      421 atgtgaagac aaccttgtat tactaataat acaagtattc taaaattatt ttcatattct
      481 cagaaccagt ttggcatttt tctcttggta gtttttgtct gcaaataaaa ttggtatttg
      541 aaaccacagc actcataagc ctgagtgtcc acttattcag tggcagtgat accctgctgc
      601 tgcccccttc ctgcacctca agatctgaac ttgtaacaat ttcattcaaa gagataacag
      661 tctatctctt acattttcat catctaactt ttatctcatg aaaatatttc ttctccagtc
      721 tgcctttata catgtagaat ggtaatgtaa tttctaaagt atatacagaa gcattccaga
      781 aattaattgc tattttaaat aatcatttta tctcaaaaaa taagtaaatc attttaacag
      841 ttatgctttt tctcagttat atattcagca tgctaaagag gacgaaactc gttatcataa
      901 tgaaatgaag tcttgggaag aacaaatgat tgaagttgga cgaaaggatc ttctacgtcg
      961 cacaataaag aaacaacgaa aatatggtgc tgaagagtgt ggaggaggca gcgtgagcaa
     1021 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     1081 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     1141 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     1201 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     1261 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     1321 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     1381 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     1441 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     1501 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     1561 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     1621 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1681 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa agtagaagat
     1741 tgagatgtgt tcacaatgga tagccacagg aaacgagtta ggtctcaata cctgaagcta
     1801 tcgtaaaatt aagaaaggat aaagttggta aaccttttat atttagtatc tttttattca
     1861 gctcatggac ttctgccagc ataatacttg ctttggaaaa cccagataaa ggttcatgca
     1921 aactttattt tgtgtttagg aactactgag gatcagagta atccaagcaa atgtgaatca
     1981 ttttaccttt gacaaaggta aatcagacta tgaagttttt tttatacagg atgatgacta
     2041 tggaaagagt actcttgttt ccttatatta tggaggcagg agtttcgttt tcaaaattgt
     2101 tacaaattgt agaagccacg gtgttctgtg atataagtgt gtgtttttca taaagcaggc
     2161 agaactcatc taggtaaatt acagttccta ggtataattc acattgtatt cagagttgat
     2221 ggttgtacat ataagtgatt gctggtttta gttgcaactt tgtataaaag ggactgagaa
     2281 atttataaac ttttttctta ctgtcttttt tctaaagtaa aaacaaagaa attatgtgcc
     2341 agatttatgc atattatttt atgttgcata gaataaaatt tttaatcttt aattttacat
     2401 ttcctaaata tattttaaga cgaaacattt gttctatagc ttttcccttt ttttaagtaa
     2461 ggaattttat ttttttctga attattttct ctcgtgagta tattgatcca gaaagaaaac
     2521 ttgtattatg tgtgttttaa aatgagaaat ctaaaaaacg aaaagtctcc aaagtctctg
     2581 gaatttgaaa cactttgcat aacgtataaa agcctgttta agagacagcc aactatggcc
     2641 tgtggatcaa atccagcctg ctgcctgctt tttatggcct gtgagctagg aattgtgttt
     2701 ataattttaa atgttttttt ttaaag
//
