LOCUS       sequence-309003-       10493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Kuo J, Yuan R, Sanchez C, Paulsson J, Silver PA
  TITLE     Toward a translationally independent RNA-based synthetic oscillator 
            using deactivated CRISPR-Cas.
  JOURNAL   Nucleic Acids Res. 2020 Aug 20;48(14):8165-8177. doi: 
            10.1093/nar/gkaa557.
  PUBMED    32609820
REFERENCE   2  (bases 1 to 10493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-08-20- 20"; volume: "48"; issue: 
            "14"; pages: "8165-8177"
COMMENT     Sequence Label: pJK520
FEATURES             Location/Qualifiers
     source          1..10493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..660)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     575..594
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(661..763)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(793..1419)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     1324..1343
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        1435..1490
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    1471..1489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             1508..1519
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(5624..5658)
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      complement(6146..6173)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(6189..6260)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(7000..7034)
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     terminator      7052..7095
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(7099..7117)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(7642..8592)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      8640..8862
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     misc_RNA        9407..9456
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             9486..9497
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             9506..10219
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             9506..10219
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(9536..9564)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10159..10179
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      10280..10366
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(10379..10473)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
       61 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      121 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      181 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
      241 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
      301 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
      361 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
      421 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
      481 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
      541 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
      601 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
      661 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
      721 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
      781 gatatcgacg tcttaagacc cactttcaca tttaagttgt ttttctaatc cgcatatgat
      841 caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc
      901 ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac
      961 ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc
     1021 atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag
     1081 agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg
     1141 acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc
     1201 cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc
     1261 gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt
     1321 ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc
     1381 gctgttaatc actttacttt tatctaatct agacatcatt aattcctaat ttttgttgac
     1441 actctatcgt tgatagagtt attttaccac tccctatcag tgatagagaa aagaattcaa
     1501 aagatctaaa gaggagaaag gatctatgtc aatttatcaa gaatttgtta ataaatatag
     1561 tttaagtaaa actctaagat ttgagttaat cccacagggt aaaacacttg aaaacataaa
     1621 agcaagaggt ttgattttag atgatgagaa aagagctaaa gactacaaaa aggctaaaca
     1681 aataattgat aaatatcatc agttttttat agaggagata ttaagttcgg tttgtattag
     1741 cgaagattta ttacaaaact attctgatgt ttattttaaa cttaaaaaga gtgatgatga
     1801 taatctacaa aaagatttta aaagtgcaaa agatacgata aagaaacaaa tatctgaata
     1861 tataaaggac tcagagaaat ttaagaattt gtttaatcaa aaccttatcg atgctaaaaa
     1921 agggcaagag tcagatttaa ttctatggct aaagcaatct aaggataatg gtatagaact
     1981 atttaaagcc aatagtgata tcacagatat agatgaggcg ttagaaataa tcaaatcttt
     2041 taaaggttgg acaacttatt ttaagggttt tcatgaaaat agaaaaaatg tttatagtag
     2101 caatgatatt cctacatcta ttatttatag gatagtagat gataatttgc ctaaatttct
     2161 agaaaataaa gctaagtatg agagtttaaa agacaaagct ccagaagcta taaactatga
     2221 acaaattaaa aaagatttgg cagaagagct aacctttgat attgactaca aaacatctga
     2281 agttaatcaa agagtttttt cacttgatga agtttttgag atagcaaact ttaataatta
     2341 tctaaatcaa agtggtatta ctaaatttaa tactattatt ggtggtaaat ttgtaaatgg
     2401 tgaaaataca aagagaaaag gtataaatga atatataaat ctatactcac agcaaataaa
     2461 tgataaaaca ctcaaaaaat ataaaatgag tgttttattt aagcaaattt taagtgatac
     2521 agaatctaaa tcttttgtaa ttgataagtt agaagatgat agtgatgtag ttacaacgat
     2581 gcaaagtttt tatgagcaaa tagcagcttt taaaacagta gaagaaaaat ctattaaaga
     2641 aacactatct ttattatttg atgatttaaa agctcaaaaa cttgatttga gtaaaattta
     2701 ttttaaaaat gataaatctc ttactgatct atcacaacaa gtttttgatg attatagtgt
     2761 tattggtaca gcggtactag aatatataac tcaacaaata gcacctaaaa atcttgataa
     2821 ccctagtaag aaagagcaag aattaatagc caaaaaaact gaaaaagcaa aatacttatc
     2881 tctagaaact ataaagcttg ccttagaaga atttaataag catagagata tagataaaca
     2941 gtgtaggttt gaagaaatac ttgcaaactt tgcggctatt ccgatgatat ttgatgaaat
     3001 agctcaaaac aaagacaatt tggcacagat atctatcaaa tatcaaaatc aaggtaaaaa
     3061 agacctactt caagctagtg cggaagatga tgttaaagct atcaaggatc ttttagatca
     3121 aactaataat ctcttacata aactaaaaat atttcatatt agtcagtcag aagataaggc
     3181 aaatatttta gacaaggatg agcattttta tctagtattt gaggagtgct actttgagct
     3241 agcgaatata gtgcctcttt ataacaaaat tagaaactat ataactcaaa agccatatag
     3301 tgatgagaaa tttaagctca attttgagaa ctcgactttg gctaatggtt gggataaaaa
     3361 taaagagcct gacaatacgg caattttatt tatcaaagat gataaatatt atctgggtgt
     3421 gatgaataag aaaaataaca aaatatttga tgataaagct atcaaagaaa ataaaggcga
     3481 gggttataaa aaaattgttt ataaactttt acctggcgca aataaaatgt tacctaaggt
     3541 tttcttttct gctaaatcta taaaatttta taatcctagt gaagatatac ttagaataag
     3601 aaatcattcc acacatacaa aaaatggtag tcctcaaaaa ggatatgaaa aatttgagtt
     3661 taatattgaa gattgccgaa aatttataga tttttataaa cagtctataa gtaagcatcc
     3721 ggagtggaaa gattttggat ttagattttc tgatactcaa agatataatt ctatagatga
     3781 attttataga gaagttgaaa atcaaggcta caaactaact tttgaaaata tatcagagag
     3841 ctatattgat agcgtagtta atcagggtaa attgtaccta ttccaaatct ataataaaga
     3901 tttttcagct tatagcaaag ggcgaccaaa tctacatact ttatattgga aagcgctgtt
     3961 tgatgagaga aatcttcaag atgtggttta taagctaaat ggtgaggcag agctttttta
     4021 tcgtaaacaa tcaataccta aaaaaatcac tcacccagct aaagaggcaa tagctaataa
     4081 aaacaaagat aatcctaaaa aagagagtgt ttttgaatat gatttaatca aagataaacg
     4141 ctttactgaa gataagtttt tctttcactg tcctattaca atcaatttta aatctagtgg
     4201 agctaataag tttaatgatg aaatcaattt attgctaaaa gaaaaagcaa atgatgttca
     4261 tatattaagt atagctagag gtgaaagaca tttagcttac tatactttgg tagatggtaa
     4321 aggcaatatc atcaaacaag atactttcaa catcattggt aatgatagaa tgaaaacaaa
     4381 ctaccatgat aagcttgctg caatagagaa agatagggat tcagctagga aagactggaa
     4441 aaagataaat aacatcaaag agatgaaaga gggctatcta tctcaggtag ttcatgaaat
     4501 agctaagcta gttatagagt ataatgctat tgtggttttt gaggatttaa attttggatt
     4561 taaaagaggg cgtttcaagg tagagaagca ggtctatcaa aagttagaaa aaatgctaat
     4621 tgagaaacta aactatctag ttttcaaaga taatgagttt gataaaactg ggggagtgct
     4681 tagagcttat cagctaacag caccttttga gacttttaaa aagatgggta aacaaacagg
     4741 tattatctac tatgtaccag ctggttttac ttcaaaaatt tgtcctgtaa ctggttttgt
     4801 aaatcagtta tatcctaagt atgaaagtgt cagcaaatct caagagttct ttagtaagtt
     4861 tgacaagatt tgttataacc ttgataaggg ctattttgag tttagttttg attataaaaa
     4921 ctttggtgac aaggctgcca aaggcaagtg gactatagct agctttggga gtagattgat
     4981 taactttaga aattcagata aaaatcataa ttgggatact cgagaagttt atccaactaa
     5041 agagttggag aaattgctaa aagattattc tatcgaatat gggcatggcg aatgtatcaa
     5101 agcagctatt tgcggtgaga gcgacaaaaa gttttttgct aagctaacta gtgtcctaaa
     5161 tactatctta caaatgcgta actcaaaaac aggtactgag ttagattatc taatttcacc
     5221 agtagcagat gtaaatggca atttctttga ttcgcgacag gcgccaaaaa atatgcctca
     5281 agatgctgat gccaatggtg cttatcatat tgggctaaaa ggtctgatgc tactaggtag
     5341 gatcaaaaat aatcaagagg gcaaaaaact caatttggtt atcaaaaatg aagagtattt
     5401 tgagttcgtg cagaatagga ataactaatt gagaagagaa aagaaaaccg ccgatcctgt
     5461 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaatt
     5521 aattaaggac caaaacgaaa aaagacgctt ttcagcgtct tattgttcgt ctttggtacc
     5581 gagaccagct agttgtggag gtgatctaca acagtagaaa ttagctagca taatacctag
     5641 gactgagcta ggtgtaaaat cctatcttaa acaaaattat ttgtagaggt cataagtttc
     5701 gtcgcatttc agcgactcat cagtgggctt agcccagact tatgactgga tccggaccaa
     5761 aacgaaaaaa gacgctcgaa agcgtctctt ttctggaatt tggtaccgag acccgtgtga
     5821 cccgttggtg atctacaaca gtagaaatta gctagcataa taccagctag ttgtggaggt
     5881 gtaaaaagct taaacaaaat tatttgtaga ggactgtttc ggcccttttg ggccatcgtc
     5941 aggtcggata cacatccggc gacagtctgg accaaaacga aaaaaggccc ccctttcggg
     6001 aggcctcttt tctggaattt ggtaccgaga cctaggactg agctaggtga tctacaacag
     6061 tagaaattaa ccttaacgat acggtacgtt tcgtatcatg tcaattggta acgaatcaga
     6121 ttccaccgta cgtcgcgtat ataaacgcag aaaggcccac ccgaaggtga gccagtgtga
     6181 ctctagtaga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     6241 tgagcctttc gttttatttg atgcctgggg gtatgggccc tagttaacgc tgtgtacccg
     6301 gacaaacacc attaatcagc aggaaggtaa attcttcaat atcctgttca acggtcagct
     6361 gttcggtcag caggcgatac caacaaaaac caaaaatcat atccagcagc agttcacgat
     6421 tggtatcttt cggcagttca ccattgctaa tggcattttc aaccagtttt ttcggcatct
     6481 cacgacgacg ttccataaac tgatctttca gctgggtcag ggttgcaggg tccagctgtg
     6541 cttctgcaat aacacaacga aatgcttcac cacaaatggt ttcacgccaa actttccaca
     6601 gattacgcag cagaaaatcc agatcggctt taaagctacc cagatccgga aatttacgca
     6661 cctgttcgct ttcattttca tacacttcgg caatcagtgc tgctttattg gtccaccaac
     6721 gataaatggt cggtttgctt gcaccggcac gacgtgcaac gctttcaatg ctcagaccgc
     6781 tataaccaca ttctttcagg atttcaatgg tgctggtcag aattgcttta tgggtatgcg
     6841 gactacgcag gctaccaatg ctgctacggc tcggggtacg tgccatctag tatttctccc
     6901 tttcaaacat agtccatagt taaacaaaat tatttgtaga ggtacgtttc ggccctaccg
     6961 ggccatcttc agtgagcggg tatcacgctc agacgtactg ctagcatagt acctaggact
     7021 gagctagccg taaaagatct ttagaattcc agaaatcatc cttagcgaaa gctaaggatt
     7081 ttttttatct gaaattctgc ctcgtgatac gcctattaat taagtacggg ttttgctgcc
     7141 cgcaaacggg ctgttctggt gttgctagtt tgttatcaga atcgcagatc cggcttcagc
     7201 cggtttgccg gctgaaagcg ctatttcttc cagaattgcc atgatttttt ccccacggga
     7261 ggcgtcactg gctcccgtgt tgtcggcagc tttgattcga taagcagcat cgcctgtttc
     7321 aggctgtcta tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc aatttcatgt
     7381 tctagttgct ttgttttact ggtttcacct gttctattag gtgttacatg ctgttcatct
     7441 gttacattgt cgatctgttc atggtgaaca gctttgaatg caccaaaaac tcgtaaaagc
     7501 tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt
     7561 gatatgtaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata
     7621 gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt
     7681 ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatact tactttgcat
     7741 gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg
     7801 tagtgttttt cttagtccgt tatgtaggta ggaatctgat gtaatggttg ttggtatttt
     7861 gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc
     7921 tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt
     7981 catattgctg taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct
     8041 tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat
     8101 ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat
     8161 cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt
     8221 ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct
     8281 tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct
     8341 gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt
     8401 ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc
     8461 tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt
     8521 ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac
     8581 taattcagac atacatctca attggtctag gtgattttaa tcactatacc aattgagatg
     8641 ggctagtcaa tgataattac tagtcctttt cctttgagtt gtgggtatct gtaaattctg
     8701 ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc
     8761 tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata
     8821 aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc
     8881 gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa
     8941 ccctaaaggc ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt
     9001 ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca
     9061 cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg attcatgcaa
     9121 ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg
     9181 gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc
     9241 cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt
     9301 aaggcagcgg tatcatcaac aggcttaccc gtcttactgt ccctagtgct tggcctagta
     9361 tgcgtctcct ttacacctcc acaactagct ggtattatgc tagcagctgt caccggatgt
     9421 gctttccggt ctgatgagtc cgtgaggacg aaacagcctc tacaaataat tttgtttaac
     9481 tttacaaaga ggagaaaggt accgcatgag taaaggagaa gaacttttca ctggagttgt
     9541 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
     9601 gggtgaaggt gatgcaacat acggaaaact tacccttaaa ttgatttgca ctactggaaa
     9661 actacctgtt ccatggccaa cacttgtcac tactttgggt tatggtgttc aatgctttgc
     9721 gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta
     9781 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
     9841 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
     9901 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
     9961 cacggcagac aaacaaaaga atggaatcaa agcgaacttc aaaattagac acaacattga
    10021 agatggaggt gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
    10081 tgtcctttta ccagacaacc attacctgtc ctaccaatct aagctttcga aagatcccaa
    10141 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
    10201 catggatgaa ctatacaaat aagcttgata tcgaattcct gcagcccggg ggatcccatt
    10261 gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
    10321 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaattcta gtgcttggat
    10381 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
    10441 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaa
//
