LOCUS       sequence-309008-       10503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   Kuo J, Yuan R, Sanchez C, Paulsson J, Silver PA
  TITLE     Toward a translationally independent RNA-based synthetic oscillator 
            using deactivated CRISPR-Cas.
  JOURNAL   Nucleic Acids Res. 2020 Aug 20;48(14):8165-8177. doi: 
            10.1093/nar/gkaa557.
  PUBMED    32609820
REFERENCE   2  (bases 1 to 10503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-08-20- 20"; volume: "48"; issue: 
            "14"; pages: "8165-8177"
COMMENT     Sequence Label: pJK526
FEATURES             Location/Qualifiers
     source          1..10503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..660)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     575..594
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(661..763)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(793..1419)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     1324..1343
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        1435..1490
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    1471..1489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             1508..1519
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(5700..5727)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(5743..5814)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(6554..6588)
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     terminator      7062..7105
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(7109..7127)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(7652..8602)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      8650..8872
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     misc_RNA        9417..9466
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             9496..9507
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             9516..10229
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             9516..10229
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(9546..9574)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10169..10189
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      10290..10376
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(10389..10483)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
       61 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      121 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      181 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
      241 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
      301 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
      361 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
      421 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
      481 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
      541 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
      601 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
      661 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
      721 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
      781 gatatcgacg tcttaagacc cactttcaca tttaagttgt ttttctaatc cgcatatgat
      841 caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc
      901 ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac
      961 ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc
     1021 atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag
     1081 agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg
     1141 acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc
     1201 cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc
     1261 gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt
     1321 ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc
     1381 gctgttaatc actttacttt tatctaatct agacatcatt aattcctaat ttttgttgac
     1441 actctatcgt tgatagagtt attttaccac tccctatcag tgatagagaa aagaattcaa
     1501 aagatctaaa gaggagaaag gatctatgtc aatttatcaa gaatttgtta ataaatatag
     1561 tttaagtaaa actctaagat ttgagttaat cccacagggt aaaacacttg aaaacataaa
     1621 agcaagaggt ttgattttag atgatgagaa aagagctaaa gactacaaaa aggctaaaca
     1681 aataattgat aaatatcatc agttttttat agaggagata ttaagttcgg tttgtattag
     1741 cgaagattta ttacaaaact attctgatgt ttattttaaa cttaaaaaga gtgatgatga
     1801 taatctacaa aaagatttta aaagtgcaaa agatacgata aagaaacaaa tatctgaata
     1861 tataaaggac tcagagaaat ttaagaattt gtttaatcaa aaccttatcg atgctaaaaa
     1921 agggcaagag tcagatttaa ttctatggct aaagcaatct aaggataatg gtatagaact
     1981 atttaaagcc aatagtgata tcacagatat agatgaggcg ttagaaataa tcaaatcttt
     2041 taaaggttgg acaacttatt ttaagggttt tcatgaaaat agaaaaaatg tttatagtag
     2101 caatgatatt cctacatcta ttatttatag gatagtagat gataatttgc ctaaatttct
     2161 agaaaataaa gctaagtatg agagtttaaa agacaaagct ccagaagcta taaactatga
     2221 acaaattaaa aaagatttgg cagaagagct aacctttgat attgactaca aaacatctga
     2281 agttaatcaa agagtttttt cacttgatga agtttttgag atagcaaact ttaataatta
     2341 tctaaatcaa agtggtatta ctaaatttaa tactattatt ggtggtaaat ttgtaaatgg
     2401 tgaaaataca aagagaaaag gtataaatga atatataaat ctatactcac agcaaataaa
     2461 tgataaaaca ctcaaaaaat ataaaatgag tgttttattt aagcaaattt taagtgatac
     2521 agaatctaaa tcttttgtaa ttgataagtt agaagatgat agtgatgtag ttacaacgat
     2581 gcaaagtttt tatgagcaaa tagcagcttt taaaacagta gaagaaaaat ctattaaaga
     2641 aacactatct ttattatttg atgatttaaa agctcaaaaa cttgatttga gtaaaattta
     2701 ttttaaaaat gataaatctc ttactgatct atcacaacaa gtttttgatg attatagtgt
     2761 tattggtaca gcggtactag aatatataac tcaacaaata gcacctaaaa atcttgataa
     2821 ccctagtaag aaagagcaag aattaatagc caaaaaaact gaaaaagcaa aatacttatc
     2881 tctagaaact ataaagcttg ccttagaaga atttaataag catagagata tagataaaca
     2941 gtgtaggttt gaagaaatac ttgcaaactt tgcggctatt ccgatgatat ttgatgaaat
     3001 agctcaaaac aaagacaatt tggcacagat atctatcaaa tatcaaaatc aaggtaaaaa
     3061 agacctactt caagctagtg cggaagatga tgttaaagct atcaaggatc ttttagatca
     3121 aactaataat ctcttacata aactaaaaat atttcatatt agtcagtcag aagataaggc
     3181 aaatatttta gacaaggatg agcattttta tctagtattt gaggagtgct actttgagct
     3241 agcgaatata gtgcctcttt ataacaaaat tagaaactat ataactcaaa agccatatag
     3301 tgatgagaaa tttaagctca attttgagaa ctcgactttg gctaatggtt gggataaaaa
     3361 taaagagcct gacaatacgg caattttatt tatcaaagat gataaatatt atctgggtgt
     3421 gatgaataag aaaaataaca aaatatttga tgataaagct atcaaagaaa ataaaggcga
     3481 gggttataaa aaaattgttt ataaactttt acctggcgca aataaaatgt tacctaaggt
     3541 tttcttttct gctaaatcta taaaatttta taatcctagt gaagatatac ttagaataag
     3601 aaatcattcc acacatacaa aaaatggtag tcctcaaaaa ggatatgaaa aatttgagtt
     3661 taatattgaa gattgccgaa aatttataga tttttataaa cagtctataa gtaagcatcc
     3721 ggagtggaaa gattttggat ttagattttc tgatactcaa agatataatt ctatagatga
     3781 attttataga gaagttgaaa atcaaggcta caaactaact tttgaaaata tatcagagag
     3841 ctatattgat agcgtagtta atcagggtaa attgtaccta ttccaaatct ataataaaga
     3901 tttttcagct tatagcaaag ggcgaccaaa tctacatact ttatattgga aagcgctgtt
     3961 tgatgagaga aatcttcaag atgtggttta taagctaaat ggtgaggcag agctttttta
     4021 tcgtaaacaa tcaataccta aaaaaatcac tcacccagct aaagaggcaa tagctaataa
     4081 aaacaaagat aatcctaaaa aagagagtgt ttttgaatat gatttaatca aagataaacg
     4141 ctttactgaa gataagtttt tctttcactg tcctattaca atcaatttta aatctagtgg
     4201 agctaataag tttaatgatg aaatcaattt attgctaaaa gaaaaagcaa atgatgttca
     4261 tatattaagt atagctagag gtgaaagaca tttagcttac tatactttgg tagatggtaa
     4321 aggcaatatc atcaaacaag atactttcaa catcattggt aatgatagaa tgaaaacaaa
     4381 ctaccatgat aagcttgctg caatagagaa agatagggat tcagctagga aagactggaa
     4441 aaagataaat aacatcaaag agatgaaaga gggctatcta tctcaggtag ttcatgaaat
     4501 agctaagcta gttatagagt ataatgctat tgtggttttt gaggatttaa attttggatt
     4561 taaaagaggg cgtttcaagg tagagaagca ggtctatcaa aagttagaaa aaatgctaat
     4621 tgagaaacta aactatctag ttttcaaaga taatgagttt gataaaactg ggggagtgct
     4681 tagagcttat cagctaacag caccttttga gacttttaaa aagatgggta aacaaacagg
     4741 tattatctac tatgtaccag ctggttttac ttcaaaaatt tgtcctgtaa ctggttttgt
     4801 aaatcagtta tatcctaagt atgaaagtgt cagcaaatct caagagttct ttagtaagtt
     4861 tgacaagatt tgttataacc ttgataaggg ctattttgag tttagttttg attataaaaa
     4921 ctttggtgac aaggctgcca aaggcaagtg gactatagct agctttggga gtagattgat
     4981 taactttaga aattcagata aaaatcataa ttgggatact cgagaagttt atccaactaa
     5041 agagttggag aaattgctaa aagattattc tatcgaatat gggcatggcg aatgtatcaa
     5101 agcagctatt tgcggtgaga gcgacaaaaa gttttttgct aagctaacta gtgtcctaaa
     5161 tactatctta caaatgcgta actcaaaaac aggtactgag ttagattatc taatttcacc
     5221 agtagcagat gtaaatggca atttctttga ttcgcgacag gcgccaaaaa atatgcctca
     5281 agatgctgat gccaatggtg cttatcatat tgggctaaaa ggtctgatgc tactaggtag
     5341 gatcaaaaat aatcaagagg gcaaaaaact caatttggtt atcaaaaatg aagagtattt
     5401 tgagttcgtg cagaatagga ataactaatt gagaagagaa aagaaaaccg ccgatcctgt
     5461 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaatt
     5521 aattaaggac caaaacgaaa aaagacgctt ttcagcgtct tattgttcgt ctttggtacc
     5581 gagaccagct agttgtggag gtgatctaca acagtagaaa ttaaccttaa cgatacggta
     5641 cgtttcgtat catgtcaatt ggtaacgaat cagattccac cgtacgtcgc gtatataaac
     5701 gcagaaaggc ccacccgaag gtgagccagt gtgactctag tagagagcgt tcaccgacaa
     5761 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
     5821 gggggtatgg gccctagtta acgctgtgta cccggacaaa caccattaat cagcaggaag
     5881 gtaaattctt caatatcctg ttcaacggtc agctgttcgg tcagcaggcg ataccaacaa
     5941 aaaccaaaaa tcatatccag cagcagttca cgattggtat ctttcggcag ttcaccattg
     6001 ctaatggcat tttcaaccag ttttttcggc atctcacgac gacgttccat aaactgatct
     6061 ttcagctggg tcagggttgc agggtccagc tgtgcttctg caataacaca acgaaatgct
     6121 tcaccacaaa tggtttcacg ccaaactttc cacagattac gcagcagaaa atccagatcg
     6181 gctttaaagc tacccagatc cggaaattta cgcacctgtt cgctttcatt ttcatacact
     6241 tcggcaatca gtgctgcttt attggtccac caacgataaa tggtcggttt gcttgcaccg
     6301 gcacgacgtg caacgctttc aatgctcaga ccgctataac cacattcttt caggatttca
     6361 atggtgctgg tcagaattgc tttatgggta tgcggactac gcaggctacc aatgctgcta
     6421 cggctcgggg tacgtgccat ctagtatttc tccctttcaa acatagtcca tagttaaaca
     6481 aaattatttg tagaggtacg tttcggccct accgggccat cttcagtgag cgggtatcac
     6541 gctcagacgt actgctagca tagtacctag gactgagcta gccgtaaaat cctatcttaa
     6601 acaaaattat ttgtagaggt cataagtttc gtcgcatttc agcgactcat cagtgggctt
     6661 agcccagact tatgactgga tccggaccaa aacgaaaaaa gacgctcgaa agcgtctctt
     6721 ttctggaatt tggtaccgag acccgtgtga cccgttggtg atctacaaca gtagaaatta
     6781 gctagcataa taccagctag ttgtggaggt gtaaaaagct taaacaaaat tatttgtaga
     6841 ggactgtttc ggcccttttg ggccatcgtc aggtcggata cacatccggc gacagtctgg
     6901 accaaaacga aaaaaggccc ccctttcggg aggcctcttt tctggaattt ggtaccgaga
     6961 cctaggactg agctaggtga tctacaacag tagaaattag ctagcataat acccgtgtga
     7021 cccgttggtg taaaatgatt tctgagatct ttagaattcc agaaatcatc cttagcgaaa
     7081 gctaaggatt ttttttatct gaaattctgc ctcgtgatac gcctattaat taagtacggg
     7141 ttttgctgcc cgcaaacggg ctgttctggt gttgctagtt tgttatcaga atcgcagatc
     7201 cggcttcagc cggtttgccg gctgaaagcg ctatttcttc cagaattgcc atgatttttt
     7261 ccccacggga ggcgtcactg gctcccgtgt tgtcggcagc tttgattcga taagcagcat
     7321 cgcctgtttc aggctgtcta tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc
     7381 aatttcatgt tctagttgct ttgttttact ggtttcacct gttctattag gtgttacatg
     7441 ctgttcatct gttacattgt cgatctgttc atggtgaaca gctttgaatg caccaaaaac
     7501 tcgtaaaagc tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atatggacag
     7561 ttttcccttt gatatgtaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc
     7621 ttcactgata gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt
     7681 tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatact
     7741 tactttgcat gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta
     7801 aagcatcgtg tagtgttttt cttagtccgt tatgtaggta ggaatctgat gtaatggttg
     7861 ttggtatttt gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca
     7921 tttgtctatc tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa
     7981 ccaccaattt catattgctg taagtgttta aatctttact tattggtttc aaaacccatt
     8041 ggttaagcct tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat
     8101 caaggctaat ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc
     8161 actcataaat cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt
     8221 ttatgaattt ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta
     8281 atttttcgct tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca
     8341 aaggattcct gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac
     8401 cataagcatt ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata
     8461 ccgtccgttc tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata
     8521 aaattagctt ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt
     8581 tgaaaacaac taattcagac atacatctca attggtctag gtgattttaa tcactatacc
     8641 aattgagatg ggctagtcaa tgataattac tagtcctttt cctttgagtt gtgggtatct
     8701 gtaaattctg ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt
     8761 ccgctagacc tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa
     8821 agaaagaata aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt
     8881 agtcagttcc gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca
     8941 gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata
     9001 ttccttttgt ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc
     9061 gctgcgctca cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg
     9121 attcatgcaa ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt
     9181 tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc
     9241 tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa
     9301 tgcacccagt aaggcagcgg tatcatcaac aggcttaccc gtcttactgt ccctagtgct
     9361 tggcctagta tgcgtctcct ttacacctcc acaactagct ggtattatgc tagcagctgt
     9421 caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc tacaaataat
     9481 tttgtttaac tttacaaaga ggagaaaggt accgcatgag taaaggagaa gaacttttca
     9541 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     9601 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa ttgatttgca
     9661 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttgggt tatggtgttc
     9721 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
     9781 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     9841 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     9901 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     9961 atgtatacat cacggcagac aaacaaaaga atggaatcaa agcgaacttc aaaattagac
    10021 acaacattga agatggaggt gttcaactag cagaccatta tcaacaaaat actccaattg
    10081 gcgatggccc tgtcctttta ccagacaacc attacctgtc ctaccaatct aagctttcga
    10141 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
    10201 ttacacatgg catggatgaa ctatacaaat aagcttgata tcgaattcct gcagcccggg
    10261 ggatcccatt gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag tcgaaagact
    10321 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaattcta
    10381 gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
    10441 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
    10501 aaa
//
