LOCUS       sequence-30901-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Carroll P, Muttucumaru DG, Parish T
  TITLE     Use of a tetracycline-inducible system for conditional expression in
            Mycobacterium tuberculosis and Mycobacterium smegmatis.
  JOURNAL   Appl Environ Microbiol. 2005 Jun . 71(6):3077-84.
  PUBMED    15933004
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2005 Jun . 71(6):3077-84."
COMMENT     Sequence Label: pHLEGM3
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    100..118
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    456..474
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(572..591)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 aagtcttctc ctttactcat atgtatatct ccttcttaaa ggatccccgg gtaccgagct
       61 cgaattcgag ctcggtacca tcaagcttat tttaattata ctctatcaat gatagagtgt
      121 caatattttt tttagttttt catgaactcg aggggatctg gctcgcaccg cggtggcggc
      181 cgctctagac gacacatgcc cagacactgc ggaaatgcca ccttcaggcc gtcgcgtcgg
      241 tcccgaattg gccgtgaacg accgccggat aagggtttcg gcggtgcgct tgatgcgggt
      301 ggacgcccga agttgtggtt gactacacga gcactgccgg gcccagcgcc tgcagtctga
      361 cctaattcag gatgcgccca aacatgcatg gatgcgttga gatgaggatg agggaagcaa
      421 gaatggatcc aaataaaaaa ctagtttgac aaataactct atcaatgata gagtgtcaac
      481 aaaaaggagg aattaatgat gtctagatta gataaaagta aagtgattaa cagcgcatta
      541 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      601 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      661 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      721 tggcangatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      781 gatggagc
//
