LOCUS       sequence-309010-        3326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multi-cassette for SapTrap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1822
FEATURES             Location/Qualifiers
     source          1..3326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     87..104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(263..290)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(382..468)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     517..539
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     531..548
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     532..548
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1562..1581)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1563..1581)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1586..1602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1586..1602)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1704..1723)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1715..2524
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1782..1801)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2671..3259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3160..3179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
       61 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      121 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      181 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
      241 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
      301 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
      361 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
      421 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
      481 tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga
      541 cggccagtct taagctcggg ccccaaataa tgctcttcgg cgggtggcgg tggatcggga
      601 ggaggaggtt cgggtggcgg aggcagtgga ggtggcggaa gtggcggcgg tggttcagga
      661 ggaggcggat ccatgagtaa aggagaagaa ttgttcactg gagttgtccc aatcctcgtc
      721 gagctcgacg gagacgtcaa cggacacaag ttctccgtct ccggagaggg agagggagac
      781 gccacctacg gaaagctcac cctcaagttc atctgcacca ccggaaagct cccagtccca
      841 tggccaaccc tcgtcaccac cttctgctac ggagtccaat gcttctcccg ttacccagac
      901 cacatgaagc gtcacgactt cttcaagtcc gccatgccag agggatacgt ccaagagcgt
      961 accatcttct tcaaggtaag tttaaacata tatatactaa ctactgatta tttaaatttt
     1021 caggacgacg gaaactacaa gacccgtgcc gaggtcaagt tcgagggaga caccctcgtc
     1081 aaccgtatcg agctcaaggt aagtttaaac agttcggtac taactaacca tacatattta
     1141 aattttcagg gaatcgactt caaggaggac ggaaacatcc tcggacacaa gctcgagtac
     1201 aactacaact cccacaacgt ctacatcatg gccgacaagc aaaagaacgg aatcaaggtc
     1261 aacttcaagg taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag
     1321 atccgtcaca acatcgagga cggatccgtc caactcgccg accactacca acaaaacacc
     1381 ccaatcggag acggaccagt cctcctccca gacaaccact acctctccac ccaatccgcc
     1441 ctctccaagg acccaaacga gaagcgtgac cacatggtcc tcctcgagtt cgtcaccgcc
     1501 gccggaatca cccacggaat ggacgagctc tacaagcgaa gagcgccatc cagctgatat
     1561 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     1621 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     1681 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     1741 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     1801 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     1861 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     1921 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     1981 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     2041 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     2101 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     2161 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     2221 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     2281 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     2341 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2401 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2461 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     2521 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     2581 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     2641 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2701 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2761 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2821 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2881 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2941 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3001 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3061 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3121 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3181 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3241 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3301 ctggcctttt gctcacatgt tctttc
//
