LOCUS       sequence-309016-        9216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9216)
  AUTHORS   Parker SJ, Amendola CR, Hollinshead KER, Yu Q, Yamamoto K, 
            Encarnacion-Rosado J, Rose RE, LaRue MM, Sohn ASW, Biancur DE, Paulo
            JA, Gygi SP, Jones DR, Wang H, Philips MR, Bar-Sagi D, Mancias JD, 
            Kimmelman AC
  TITLE     Selective alanine transporter utilization creates a targetable 
            metabolic niche in pancreatic cancer.
  JOURNAL   Cancer Discov. 2020 Apr 27. pii: 2159-8290.CD-19-0959. doi: 
            10.1158/2159-8290.CD-19-0959.
  PUBMED    32341021
REFERENCE   2  (bases 1 to 9216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2020 Apr 27. pii: 2159-8290.CD-19-0959. doi: 
            10.1158/2159-8290.CD-19-0959."
COMMENT     Sequence Label: pLentiCMVblast_SLC38A2_N82A
FEATURES             Location/Qualifiers
     source          1..9216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2013..2034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2049..2054,2055..2072,2073..2079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2087..2103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2092..2114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2111..2127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2111..2127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2146..2166
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2148..2166
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2194..2420
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2421..2601
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2648..2773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3266..3499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3684..3728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3995..4111
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4134..4437
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4438..4641
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4592..4612
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4638..4662
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4746..4770
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6138..6149)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6301..6325)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6356..6944
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6409..6429)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6827..6838)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6995..7015)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7000..7329
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7180..7315
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7377..7424
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7443..7841
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7928..8161
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8233..8354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8270..8289)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8324..8343
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8394..8529
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8549..8568)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8550..8568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8578..8595)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8578..8594)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8587..8609)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8736..9191
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8823..8842)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9033..9054
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2101 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     2161 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     2221 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     2281 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     2341 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     2401 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     2461 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2521 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2581 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     2641 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     2701 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     2761 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     2821 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     2881 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     2941 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3001 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3061 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     3121 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     3181 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     3241 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     3301 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     3361 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     3421 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     3481 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     3541 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     3601 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     3661 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     3721 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     3781 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     3841 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     3901 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     3961 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     4021 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4081 tacaaaaaca aattacaaaa ttcaaaattt tatcgataag cttgggagtt ccgcgttaca
     4141 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     4201 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     4261 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     4321 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     4381 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     4441 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     4501 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     4561 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     4621 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     4681 cgctgttttg acctccatag aagacaccga ctctagtcca gtgtggtgga attctgcaga
     4741 tatcaacaag tttgtacaaa aaagcaggct tcaccatgaa gaaggccgaa atgggacgat
     4801 tcagtatttc cccggatgaa gacagcagca gctacagttc caacagcgac ttcaactact
     4861 cctaccccac caagcaagct gctctgaaaa gccattatgc agatgtagat cctgaaaacc
     4921 agaacttttt acttgaatcg aatttgggga agaagaagta tgaaacagaa tttcatccag
     4981 gtactacttc ctttggaatg tcagtattta acttgtccgc tgcaatcgtt ggttctggaa
     5041 tccttgggct ttcttatgcc atggctaata ctggaattgc tctttttata attctcttga
     5101 catttgtgtc aatattttcc ctgtattctg ttcatctcct tttgaagact gccaatgaag
     5161 gagggtcttt attatatgaa caattgggat ataaggcatt tggattagtt ggaaagcttg
     5221 cagcatctgg atcaattaca atgcagaaca ttggagctat gtcaagctac ctcttcatag
     5281 tgaaatatga gttgcctttg gtgatccagg cattaacgaa cattgaagat aaaactggat
     5341 tgtggtatct gaacgggaac tatttggttc tgttggtgtc attggtggtc attcttcctt
     5401 tgtcgctgtt tagaaattta ggatatttgg gatataccag tggcctttcc ttgttgtgta
     5461 tggtgttctt tctgattgtg gtcatttgca agaaatttca ggttccgtgt cctgtggaag
     5521 ctgctttgat aattaacgaa acaataaaca ccaccttaac acagccaaca gctcttgtac
     5581 ctgctttgtc acataacgtg actgaaaatg actcttgcag acctcactat tttattttca
     5641 actcacagac tgtctatgct gtgccaattc tgatcttttc atttgtctgt catcctgctg
     5701 ttcttcccat ctatgaagaa ctgaaagacc gcagccgtag aagaatgatg aatgtgtcca
     5761 agatttcatt ttttgctatg tttctcatgt atctgcttgc cgccctcttt ggatacctaa
     5821 cattttacga acatgttgag tcagaattgc ttcataccta ctcttctatc ttgggaactg
     5881 atattcttct tctcattgtc cgtctggctg tgttaatggc tgtgaccctg acagtaccag
     5941 tagttatttt cccaatccgg agttctgtaa ctcacttgtt gtgtgcatca aaagatttca
     6001 gttggtggcg tcatagtctc attacagtgt ctatcttggc atttaccaat ttacttgtca
     6061 tctttgtccc aactattagg gatatctttg gttttattgg tgcatctgca gcttctatgt
     6121 tgatttttat tcttccttct gccttctata tcaagttggt gaagaaagaa cctatgaaat
     6181 ctgtacaaaa gattggggct ttgttcttcc tgttaagtgg tgtactggtg atgaccggaa
     6241 gcatggcctt gattgttttg gattgggtac acaatgcacc tggaggtggc cattaataaa
     6301 acccagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgacaatca
     6361 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6421 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6481 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6541 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6601 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6661 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6721 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     6781 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6841 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6901 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttctgcagat
     6961 atccggttag taatgagttt ggaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     7021 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7081 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7141 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7201 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7261 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7321 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     7381 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     7441 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     7501 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     7561 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     7621 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     7681 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     7741 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     7801 ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacaattc gagctcggta
     7861 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7921 gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact
     7981 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8041 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8101 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8161 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     8221 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8281 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8341 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     8401 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8461 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8521 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     8581 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     8641 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     8701 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     8761 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     8821 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8881 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     8941 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9001 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9061 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9121 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     9181 gcttacaatt taggtggcac ttttcgggga aatgtg
//
