LOCUS       sequence-30902-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Carroll P, Muttucumaru DG, Parish T
  TITLE     Use of a tetracycline-inducible system for conditional expression in
            Mycobacterium tuberculosis and Mycobacterium smegmatis.
  JOURNAL   Appl Environ Microbiol. 2005 Jun . 71(6):3077-84.
  PUBMED    15933004
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2005 Jun . 71(6):3077-84."
COMMENT     Sequence Label: pHLEGM4
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    98..116
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    183..201
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(299..318)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 aagcttctcc tttactcata tgtatatctc cttcttaaag gatccccggg taccgagctc
       61 gaattcgagc tcggaccatc aagcttattt taattatact ctatcaatga tagagtgtca
      121 atattttttt tagtttttca tgaactcgag gggatccaaa taaaaaacta gtttgacaaa
      181 taactctatc aatgatagag tgtcaacaaa aaggaggaat taatgatgtc tagattagat
      241 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta
      301 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta
      361 aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact
      421 cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt
      481 tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct
      541 acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt
      601 tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta
      661 ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt
      721 atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc
      781 ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaa
//
