LOCUS       sequence-309020-       11669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant 
            cDNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Parker SJ, Amendola CR, Hollinshead KER, Yu Q, Yamamoto K, 
            Encarnacion-Rosado J, Rose RE, LaRue MM, Sohn ASW, Biancur DE, Paulo
            JA, Gygi SP, Jones DR, Wang H, Philips MR, Bar-Sagi D, Mancias JD, 
            Kimmelman AC
  TITLE     Selective alanine transporter utilization creates a targetable 
            metabolic niche in pancreatic cancer.
  JOURNAL   Cancer Discov. 2020 Apr 27. pii: 2159-8290.CD-19-0959. doi: 
            10.1158/2159-8290.CD-19-0959.
  PUBMED    32341021
REFERENCE   2  (bases 1 to 11669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2020 Apr 27. pii: 2159-8290.CD-19-0959. doi: 
            10.1158/2159-8290.CD-19-0959."
COMMENT     Sequence Label: pInducer20_SLC38A2_N82A
FEATURES             Location/Qualifiers
     source          1..11669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4093..4104)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4256..4280)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4333..4359
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4445..5656
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5493..5512
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5715..6332,6333..6338,6339..6374,6375..6377,6378..6413,
                     6414..6416,6417..6452,6453..6461)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6499..7072
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6666..6683)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6893..6912
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7088..7882
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7088..7882
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7142..7161)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7752..7771
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8059..8647
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8112..8132)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8530..8541)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8722..8955
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9074..9092)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9160..9264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9265..9333,9334..10125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9483..9502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10296..10884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10785..10804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11038..11055
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11295..11316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11331..11336,11337..11354,11355..11361)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11369..11385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11374..11396
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11393..11409
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11393..11409
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11459..11478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggcttcacc atgaagaagg ccgaaatggg acgattcagt
     2761 atttccccgg atgaagacag cagcagctac agttccaaca gcgacttcaa ctactcctac
     2821 cccaccaagc aagctgctct gaaaagccat tatgcagatg tagatcctga aaaccagaac
     2881 tttttacttg aatcgaattt ggggaagaag aagtatgaaa cagaatttca tccaggtact
     2941 acttcctttg gaatgtcagt atttaacttg tccgctgcaa tcgttggttc tggaatcctt
     3001 gggctttctt atgccatggc taatactgga attgctcttt ttataattct cttgacattt
     3061 gtgtcaatat tttccctgta ttctgttcat ctccttttga agactgccaa tgaaggaggg
     3121 tctttattat atgaacaatt gggatataag gcatttggat tagttggaaa gcttgcagca
     3181 tctggatcaa ttacaatgca gaacattgga gctatgtcaa gctacctctt catagtgaaa
     3241 tatgagttgc ctttggtgat ccaggcatta acgaacattg aagataaaac tggattgtgg
     3301 tatctgaacg ggaactattt ggttctgttg gtgtcattgg tggtcattct tcctttgtcg
     3361 ctgtttagaa atttaggata tttgggatat accagtggcc tttccttgtt gtgtatggtg
     3421 ttctttctga ttgtggtcat ttgcaagaaa tttcaggttc cgtgtcctgt ggaagctgct
     3481 ttgataatta acgaaacaat aaacaccacc ttaacacagc caacagctct tgtacctgct
     3541 ttgtcacata acgtgactga aaatgactct tgcagacctc actattttat tttcaactca
     3601 cagactgtct atgctgtgcc aattctgatc ttttcatttg tctgtcatcc tgctgttctt
     3661 cccatctatg aagaactgaa agaccgcagc cgtagaagaa tgatgaatgt gtccaagatt
     3721 tcattttttg ctatgtttct catgtatctg cttgccgccc tctttggata cctaacattt
     3781 tacgaacatg ttgagtcaga attgcttcat acctactctt ctatcttggg aactgatatt
     3841 cttcttctca ttgtccgtct ggctgtgtta atggctgtga ccctgacagt accagtagtt
     3901 attttcccaa tccggagttc tgtaactcac ttgttgtgtg catcaaaaga tttcagttgg
     3961 tggcgtcata gtctcattac agtgtctatc ttggcattta ccaatttact tgtcatcttt
     4021 gtcccaacta ttagggatat ctttggtttt attggtgcat ctgcagcttc tatgttgatt
     4081 tttattcttc cttctgcctt ctatatcaag ttggtgaaga aagaacctat gaaatctgta
     4141 caaaagattg gggctttgtt cttcctgtta agtggtgtac tggtgatgac cggaagcatg
     4201 gccttgattg ttttggattg ggtacacaat gcacctggag gtggccatta ataaaaccca
     4261 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc
     4321 ccgcggttcg aatacccata cgacgtccca gactacgctt agtaatgatt aattaaacta
     4381 gaaattctac cgggtagggg aggcgctttt cccaaggcag tctggagtaa cccacccaag
     4441 atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc
     4501 gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg
     4561 acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt
     4621 ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc
     4681 gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc
     4741 cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt
     4801 gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg
     4861 ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga
     4921 ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg
     4981 agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct
     5041 gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg
     5101 ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga
     5161 ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg
     5221 cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg
     5281 tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg
     5341 tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct
     5401 gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg
     5461 gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc
     5521 ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg
     5581 gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt
     5641 ggcttttttg ttagacgaag cttggtaccg agctcggatc tccaccccgt accggtcctg
     5701 cagtcgaatt caccatgtct agactggaca agagcaaagt cataaacgga gctctggaat
     5761 tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag
     5821 ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc
     5881 caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc
     5941 aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg
     6001 gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc
     6061 agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg
     6121 ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag
     6181 aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc
     6241 tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg
     6301 gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg
     6361 acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg
     6421 acgctcttga cgattttgac cttgacatgc tccccgggta actaagtaag gatccgcggc
     6481 cgcactagag gaattccgcc cctctccccc ccccccccct aacgttactg gccgaagccg
     6541 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     6601 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     6661 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     6721 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     6781 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     6841 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     6901 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     6961 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     7021 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttaccg
     7081 gtccaccatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     7141 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     7201 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     7261 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     7321 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     7381 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     7441 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     7501 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     7561 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     7621 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     7681 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     7741 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     7801 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     7861 ccttcttgac gagttcttct gatgtacaag taaagcggcc gcgactctag atcataatca
     7921 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     7981 acctgaaaca taaaatgaat gcaattgttg ttgtttagtc cctcccaatt cgatatcaag
     8041 cttatcgcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8101 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8161 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8221 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8281 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8341 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8401 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     8461 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8521 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8581 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8641 tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat cttagccact
     8701 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     8761 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8821 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8881 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8941 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     9001 agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct
     9061 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     9121 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     9181 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     9241 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     9301 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     9361 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     9421 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     9481 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     9541 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     9601 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     9661 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     9721 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     9781 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     9841 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     9901 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9961 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    10021 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    10081 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    10141 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    10201 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    10261 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    10321 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    10381 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    10441 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    10501 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    10561 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    10621 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    10681 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    10741 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    10801 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    10861 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    10921 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10981 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    11041 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    11101 tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg
    11161 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    11221 taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt
    11281 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    11341 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    11401 agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    11461 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
    11521 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
    11581 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
    11641 tgttaaatat gtactacaaa cttagtagt
//
