LOCUS       sequence-309024-        9232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control lentiviral plasmid encoding GFP-Nluc BRET reporter from 
            Schaub et al., Cancer Research, 2015..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Wu AY, Sung YC, Chen YJ, Chou ST, Guo V, Chien JC, Ko JJ, Yang AL, 
            Huang HC, Chuang JC, Wu S, Ho MR, Ericsson M, Lin WW, Cheung CHY, 
            Juan HF, Ueda K, Chen Y, Lai CP
  TITLE     Multiresolution Imaging Using Bioluminescence Resonance Energy 
            Transfer Identifies Distinct Biodistribution Profiles of 
            Extracellular Vesicles and Exomeres with Redirected Tropism.
  JOURNAL   Adv Sci (Weinh). 2020 Aug 16;7(19):2001467. doi: 
            10.1002/advs.202001467. eCollection 2020 Oct.
  PUBMED    33042758
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv Sci 
            (Weinh)."; date: "2020-08-16"; volume: "7(19):2001467. doi"; pages: 
            " 10.1002/advs.202001467. eCollection 2020 Oct"
COMMENT     Sequence Label: pLenti-GRET
FEATURES             Location/Qualifiers
     source          1..9232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..44
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     278..295
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    412..433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(448..453,454..471,472..478)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    486..502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     491..513
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     510..526
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     510..526
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     545..565
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        547..565
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        593..819
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             820..1000
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1047..1172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1665..1898
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2083..2127
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2510
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2533..2836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2837..3040
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2991..3011
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3037..3061
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3145..3169
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3175..3177,3178..3180,3181..3891)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3175..3177,3178..3180,3181..3891)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3220..3241)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3481..3500)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3828..3849
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3907..4422
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     protein_bind    complement(4429..4453)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4484..5072
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4537..4557)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4955..4966)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5086..5585
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5121..5144)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5518..5537
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5606..6205
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5606..5625)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6102..6122
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6343..6576
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6648..6769
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6685..6704)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6739..6758
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6809..6944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6964..6983)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6965..6983)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6993..7010)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6993..7009)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7002..7024)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7151..7606
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7238..7257)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7448..7469
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7632..7736
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7737..7805,7806..8597)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7955..7974)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8768..9232,1..124)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
       61 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      121 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      181 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      241 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      301 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      361 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      421 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      481 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      541 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
      601 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      661 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      721 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      781 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      841 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      901 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      961 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     1021 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     1081 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     1141 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1201 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1261 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1321 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1381 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1441 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1501 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1561 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1621 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1681 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1741 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1801 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1861 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1921 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1981 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     2041 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     2101 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     2161 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2221 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2281 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2341 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc
     2521 ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2581 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2641 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2701 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2761 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2821 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     2881 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     2941 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     3001 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
     3061 cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac tctagtccag
     3121 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctc caccatggtg
     3181 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3241 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3301 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3361 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3421 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3481 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3541 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3601 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3661 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3721 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3781 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3841 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggatatctcc
     3901 ggaggtatgg tcttcacact cgaagatttc gttggggact ggcgacagac agccggctac
     3961 aacctggacc aagtccttga acagggaggt gtgtccagtt tgtttcagaa tctcggggtg
     4021 tccgtaactc cgatccaaag gattgtcctg agcggtgaaa atgggctgaa gatcgacatc
     4081 catgtcatca tcccgtatga aggtctgagc ggcgaccaaa tgggccagat cgaaaaaatt
     4141 tttaaggtgg tgtaccctgt ggatgatcat cactttaagg tgatcctgca ctatggcaca
     4201 ctggtaatcg acggggttac gccgaacatg atcgactatt tcggacggcc gtatgaaggc
     4261 atcgccgtgt tcgacggcaa aaagatcact gtaacaggga ccctgtggaa cggcaacaaa
     4321 attatcgacg agcgcctgat caaccccgac ggctccctgc tgttccgagt aaccatcaac
     4381 ggagtgaccg gctggcggct gtgcgaacgc attctggcgt aattctagac ccagctttct
     4441 tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gacaatcaac ctctggatta
     4501 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     4561 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4621 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4681 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4741 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4801 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4861 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     4921 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4981 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     5041 gagtcggatc tccctttggg ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc
     5101 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     5161 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     5221 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     5281 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     5341 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     5401 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     5461 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     5521 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     5581 tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     5641 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     5701 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     5761 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     5821 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     5881 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     5941 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     6001 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     6061 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     6121 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     6181 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
     6241 gagcgcacga ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg
     6301 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact
     6361 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     6421 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6481 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6541 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     6601 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     6661 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6721 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     6781 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6841 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6901 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     6961 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     7021 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7081 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7141 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7201 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7261 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7321 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7381 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7441 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7501 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7561 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     7621 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7681 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7741 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7801 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7861 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7921 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7981 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8041 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8101 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8161 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8221 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8281 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8341 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8401 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8461 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8521 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8581 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8641 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8701 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8761 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8821 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8881 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8941 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9001 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9061 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9121 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9181 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca gg
//
