LOCUS       sequence-30904-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Karanicolas J, Corn JE, Chen I, Joachimiak LA, Dym O, Peck SH, 
            Albeck S, Unger T, Hu W, Liu G, Delbecq S, Montelione GT, Spiegel 
            CP, Liu DR, Baker D
  TITLE     A de novo protein binding pair by computational design and directed 
            evolution.
  JOURNAL   Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31.
  PUBMED    21458342
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-04-22"; volume: "42(2)"; pages: "250-60. Epub 2011 Mar 
            31"
COMMENT     Sequence Label: Prb
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             482..499
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(552..570)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      566..613
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(737..756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttccctctga atattttgtt tactttaaga aggagatata catatgggca gcacccgtcc
       61 gattgatggt ctgacggacg aagatatccg cgagattctg actcgttata aaaagatcgc
      121 tctggtgggg gcctctccga aaccggaacg cgacgcaaat attgttatga aatatctgct
      181 ggagcatggc tatgatgtct acccggtaaa cccgaattat gaagaagtgc tgggccgcaa
      241 gtgctacccg agtgttctgg acatcccgga taaaattgag gtcgtagatc tgtttgtgga
      301 cccggctaaa gcgtggcgct tcgttgtcta tgccatcaag aaaggggcaa aagtggtatg
      361 gtttcagtac aacacatatt atccgctggc tgcgcgtcaa gccaaggaag caggcctgat
      421 tatcgttgct aatcgctgta tgatgcgtga gcacgaacgc ctgctgggcg aaaaactcga
      481 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      541 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      601 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
      661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      781 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
      841 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
      901 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
      961 c
//
