LOCUS       sequence-30905-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Karanicolas J, Corn JE, Chen I, Joachimiak LA, Dym O, Peck SH, 
            Albeck S, Unger T, Hu W, Liu G, Delbecq S, Montelione GT, Spiegel 
            CP, Liu DR, Baker D
  TITLE     A de novo protein binding pair by computational design and directed 
            evolution.
  JOURNAL   Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31.
  PUBMED    21458342
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-04-22"; volume: "42(2)"; pages: "250-60. Epub 2011 Mar 
            31"
COMMENT     Sequence Label: Pdar
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..29)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             517..539
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    554..578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(686..705)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gccggatctc agtggtggtg gtggtggtgc tcgagttgca ggatctctgc cagatcttcc
       61 tgaccttgat taatcgagat atcaaacgcg gtcaggccca gtgcgtcttg ggcgttgaca
      121 tctgcgccgt gtttcagcag aacttccaca atctccagtt ggccactcag cgctgctagg
      181 tgcagcggtg tccacccggc gcggtcgtac gcattaacgt cagccccgtg cttcagcagg
      241 acttcgacaa tctccagatg gccgtcataa gcggccaggt gcagcggagt aatacccgca
      301 ctatcagaag cgttcacatc ggcgccattt ttcagcagta cctctacgat ttccagttgc
      361 ccgtttgcag ccgccaggtg cagcggagtc aggccattgt cgtcggttgc gttaacatcg
      421 gccccattcg ccatcagaat acgcacctca tcatcttggc cggccgcggc tgcttccagc
      481 agctttttac ccagatcgct gcccatcata tgtatatctc cttcttaaag ttaaacaaaa
      541 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattgaat
      601 ttcgcgggat cgagatcgat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc
      661 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
      721 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtgg
//
