LOCUS       sequence-30907-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Karanicolas J, Corn JE, Chen I, Joachimiak LA, Dym O, Peck SH, 
            Albeck S, Unger T, Hu W, Liu G, Delbecq S, Montelione GT, Spiegel 
            CP, Liu DR, Baker D
  TITLE     A de novo protein binding pair by computational design and directed 
            evolution.
  JOURNAL   Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31.
  PUBMED    21458342
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-04-22"; volume: "42(2)"; pages: "250-60. Epub 2011 Mar 
            31"
COMMENT     Sequence Label: PrbC10
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             67..89
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             109..126
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..147
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(714..732)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      728..775
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctctgtgcgg ctcggagagc tctttattaa gcggccgccc tgcaggactc gagttctaga
       61 ancaattttg tttaacttta agaaggagat atacatatga aatcttctca ccatcaccat
      121 caccatgaaa acctgtactt ccaatccaat gcaatgggat ccacccgtcc gattgatggt
      181 ctgacggacg aagatatccg cgagattctg actcgttata aaaagatcgc tctggtgggg
      241 gcctctccga aaccggaacg cgacgcaaat attgttatga aatatctgct ggagcatggc
      301 tatgatgtct acccggtaaa cccgaattat gaagaagtgc tgggccgcaa gtgctacccg
      361 agtgttctgg acatcccgga taaagttgag gtcgtagatc tgtttgtgaa cccggctaaa
      421 gcgtggcgct tcgttgtcta tgccatcaag aaaggggcaa aagtggtatg gtttcagtac
      481 aacacatatt atccgctggc tgcgcgtcaa gccaaggaag caggcccgat tatcgttgct
      541 aatcgctgta tgatgcgtga gcacgaacgc ctgctgggcg aaaaataata acattggaag
      601 tggataacgg atccgcgatc gcggcgcgcc acctggtggc cggccggtac cacgcgtgcg
      661 cgctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      721 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      781 aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga
      841 tagtggctcc
//
