LOCUS       sequence-309073-        8461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for mutated Fc-fused ACE2(740).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Glasgow A, Glasgow J, Limonta D, Solomon P, Lui I, Zhang Y, Nix MA, 
            Rettko NJ, Zha S, Yamin R, Kao K, Rosenberg OS, Ravetch JV, Wiita 
            AP, Leung KK, Lim SA, Zhou XX, Hobman TC, Kortemme T, Wells JA
  TITLE     Engineered ACE2 receptor traps potently neutralize SARS-CoV-2.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Nov 10;117(45):28046-28055. doi: 
            10.1073/pnas.2016093117. Epub 2020 Oct 22.
  PUBMED    33093202
REFERENCE   2  (bases 1 to 8461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2016093117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-11-10- 10"; volume: "117"; issue: "45"; pages: 
            "28046-28055"
COMMENT     Sequence Label: pCVD293
FEATURES             Location/Qualifiers
     source          1..8461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2826..2846
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             3582..3626
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     polyA_signal    3662..3783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3673..3692)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3727..3746
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    3887..4281
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(4295..4669)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        5262..5565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5645..6233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5725..5744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6341..6389
                     /note="synthetic polyadenylation signal"
     primer_bind     6440..6459
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        6510..6813
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6814..7017
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6968..6988
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7062..7081
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7062..7080
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7113..8078
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             7113..8075
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             8098..8127
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             8143..8160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8183..8200)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8189..8413
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggcga aggagggcca ccatgcgaat gcagctgctg ctgctgattg
      601 cgctgagcct ggcgctggtg accaacagca ctagtcaatc taccatcgaa gagcaggcca
      661 aaacattcct cgacttcttt aatatccagg ctgaagacct tttctaccaa tcaagtctgg
      721 ctagctggaa ttacaataca aacattacag aggagaacgt acaaaacatg aataacgcag
      781 gggacaagtg gagcgcattc cttaaggaac aaagtaccct tgcgcaaatg tatccgctgc
      841 aagagattca aaacctgacg gttaagctgc aacttcaggc cctccaacaa aatggaagtt
      901 cagtcttgtc agaagacaaa agcaagcgac tgaacaccat ccttaacacc atgtcaacca
      961 tatattcaac aggtaaagtt tgcaatccgg ataaccccca agaatgtttg cttcttgaac
     1021 ccggtctcaa cgaaattatg gccaacagtc ttgattacaa cgagcgattg tgggcatggg
     1081 aaagttggag gagtgaggta ggcaaacagt tgagacctct ttatgaagag tacgttgtcc
     1141 ttaaaaatga aatggctcgc gcgaatcatt atgaagacta tggtgactac tggagggggg
     1201 attatgaggt gaacggggtg gacggatacg attactctag gggccagctg atagaggatg
     1261 tcgagcacac ctttgaggag attaagccgt tgtacgaaca tttgcacgcc tatgtcaggg
     1321 ctaagctcat gaacgcttat ccgagttata tctccccgat aggatgcttg cctgctcact
     1381 tgttgggcga tatgtgggga cgcttttgga ccaacttgta ttcccttacg gtaccgttcg
     1441 gccagaaacc aaatatcgac gtgacagacg caatggtgga tcaagcatgg gatgcgcaac
     1501 gaatcttcaa ggaggcagaa aaatttttcg tttcagttgg actcccaaac atgacgcagg
     1561 gtttctggga gaactcaatg ttgacagatc caggtaatgt gcagaaagcg gtttgccacc
     1621 ctactgcatg ggatcttggt aaaggggact tccgcatact catgtgtacg aaagtaacta
     1681 tggacgactt tcttactgcg caccacgaga tggggcacat acaatacgat atggcgtacg
     1741 cagctcaacc tttccttctg cggaacgggg cgaatgaagg atttcacgag gcagtgggtg
     1801 agattatgtc cctgtcagct gccactccga aacatctgaa aagcatcggc ctgttgagcc
     1861 cagacttcca agaagataat gagaccgaaa taaacttcct tctgaagcaa gcactgacta
     1921 ttgtaggtac cttgcccttt acatacatgc tggagaagtg gaggtggatg gtatttaagg
     1981 gggagatacc gaaagatcaa tggatgaaaa agtggtggga aatgaaaagg gagatcgttg
     2041 gcgtagttga accagtaccg catgatgaga cgtactgcga tccggctagt ctgttccatg
     2101 tctctaatga ttactctttc atccgctact acacccgcac gctgtatcaa ttccagttcc
     2161 aagaagctct ctgtcaggct gccaagcacg aaggaccgct gcacaaatgc gacattagca
     2221 attctacaga ggcgggtcag aagttgttca atatgcttag actggggaag agcgaaccgt
     2281 ggacgctcgc tttggagaac gttgttggag ctaagaatat gaacgtcagg cccttgctga
     2341 attactttga acctctgttt acgtggttga aagaccaaaa taaaaactcc tttgttgggt
     2401 ggagtactga ctggtccccc tatgcggacc aaagcatcaa agtgaggata agcctaaaat
     2461 cagctcttgg agataaagca tatgaatgga acgacaatga aatgtacctg ttccgatcat
     2521 ctgttgcata tgctatgagg cagtactttt taaaagtaaa aaatcagatg attctttttg
     2581 gggaggagga tgtgcgagtg gctaatttga aaccaagaat ctcctttaat ttctttgtca
     2641 ctgcacctaa aaatgtgtct gatatcattc ctagaactga agttgaaaag gccatcagga
     2701 tgtcccggag ccgtatcaat gatgctttcc gtctgaatga caacagccta gagtttctgg
     2761 ggatacagcc aacacttgga cctcctaacc agccccctgt ttccactagt tctggtggtg
     2821 gtggtgagaa tctgtacttt cagagctcgg gcggaggatc gggtggaggc gagcccaaat
     2881 cttgtgacaa aactcacaca tgccctccat gcccagcgcc agaattgctg ggcggaccca
     2941 gcgtgttcct gttcccacct aaacctaaag acaccctgat gatcagccga acccctgagg
     3001 tgacctgcgt ggtggtggac gtgagccacg aggaccccga ggtgaagttc aactggtatg
     3061 tggacggcgt ggaggtccac aatgccaaaa cgaagcccag ggaggagcag tacaacagca
     3121 cctacagggt agtgagcgtc ttgaccgtgc tgcaccagga ctggctgaac ggcaaggaat
     3181 acaaatgcaa ggtcagcaat aaggctctgc cggctcctat cgagaagaca atcagcaagg
     3241 caaagggcca gccacgcgaa ccgcaggtgt atactctgcc ccccagccgg gacgagctga
     3301 ccaagaacca ggtgtccctg acctgtctgg tgaaaggctt ctaccccagc gacatcgctg
     3361 tggagtggga gagtaacggg cagcccgaga acaactacaa gaccacgcct cctgtgctgg
     3421 acagcgacgg cagcttcttc ctgtatagca agctcaccgt ggacaagagc aggtggcaac
     3481 agggcaacgt gttcagctgc tctgtgatgc acgaggccct gcacaaccat tacacccaga
     3541 agagtctcag tctgagcccg ggaaagggtg gaggcggatc cggcctgaac gacatcttcg
     3601 aggctcagaa aatcgaatgg cacgaaggct aatcccagtg tcgctagctg gccagacatg
     3661 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3721 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3781 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     3841 ttttaaagca agtaaaacct ctacaaatgt ggtatggaat taattctaaa atacagcata
     3901 gcaaaacttt aacctccaaa tcaagcctct acttgaatcc ttttctgagg gatgaataag
     3961 gcataggcat caggggctgt tgccaatgtg cattagctgt ttgcagcctc accttctttc
     4021 atggagttta agatatagtg tattttccca aggtttgaac tagctcttca tttctttatg
     4081 ttttaaatgc actgacctcc cacattccct ttttagtaaa atattcagaa ataatttaaa
     4141 tacatcattg caatgaaaat aaatgttttt tattaggcag aatccagatg ctcaaggccc
     4201 ttcataatat cccccagttt agtagttgga cttagggaac aaaggaacct ttaatagaaa
     4261 ttggacagca agaaagcgag cttctagctt atcctcagtc ctgctcctct gccacaaagt
     4321 gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga
     4381 tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact
     4441 cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca
     4501 cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt
     4561 cgtcctccac gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg
     4621 cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa cttggccatg atggctcctc
     4681 ctgtcaggag aggaaagaga agaaggttag tacaattgct atagtgagtt gtattatact
     4741 atgcagatat actatgccaa tgattaattg tcaaactagg gctgcagggt tcatagtgcc
     4801 acttttcctg cactgcccca tctcctgccc accctttccc aggcatagac agtcagtgac
     4861 ttaccaaact cacaggaggg agaaggcaga agcttgagac agacccgcgg gaccgccgaa
     4921 ctgcgagggg acgtggctag ggcggcttct tttatggtgc gccggccctc ggaggcaggg
     4981 cgctcgggga ggcctagcgg ccaatctgcg gtggcaggag gcggggccga aggccgtgcc
     5041 tgaccaatcc ggagcacata ggagtctcag ccccccgccc caaagcaagg ggaagtcacg
     5101 cgcctgtagc gccagcgtgt tgtgaaatgg gggcttgggg gggttggggc cctgattagt
     5161 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
     5221 cgctatccac gcccattgat gtactgccaa aaccgcatca tcatggtaat agcgatgact
     5281 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     5341 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     5401 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     5461 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     5521 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcctgc aggttaatta
     5581 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5641 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5701 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5761 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5821 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5881 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5941 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6001 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6061 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6121 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6181 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6241 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6301 tggtcatggc tagttaatta acatttaaat cagcggccgc aataaaatat ctttattttc
     6361 attacatctg tgtgttggtt ttttgtgtga atcgtaacta acatacgctc tccatcaaaa
     6421 caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtaatcaat tacggggtca
     6481 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     6541 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     6601 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     6661 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     6721 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     6781 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     6841 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     6901 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     6961 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     7021 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
     7081 gagacccaag ctggctagtt aagcttgcca ccatgaagga taacaccgtg ccactgaaat
     7141 tgattgccct gttagcgaac ggtgaatttc actctggcga gcagttgggt gaaacgctgg
     7201 gaatgagccg ggcggctatt aataaacaca ttcagacact gcgtgactgg ggcgttgatg
     7261 tctttaccgt tccgggtaaa ggatacagcc tgcctgagcc tatccagtta cttaatgcta
     7321 aacagatatt gggtcagctg gatggcggta gtgtagccgt gctgccagtg attgactcca
     7381 cgaatcagta ccttcttgat cgtatcggag agcttaaatc gggcgatgct tgcattgcag
     7441 aataccagca ggctggccgt ggtcgccggg gtcggaaatg gttttcgcct tttggcgcaa
     7501 acttatattt gtcgatgttc tggcgtctgg aacaaggccc ggcggcggcg attggtttaa
     7561 gtctggttat cggtatcgtg atggcggaag tattacgcaa gctgggtgca gataaagttc
     7621 gtgttaaatg gcctaatgac ctctatctgc aggatcgcaa gctggcaggc attctggtgg
     7681 agctgactgg caaaactggc gatgcggcgc aaatagtcat tggagccggg atcaacatgg
     7741 caatgcgccg tgttgaagag agtgtcgtta atcaggggtg gatcacgctg caggaagcgg
     7801 ggatcaatct cgatcgtaat acgttggcgg ccatgctaat acgtgaatta cgtgctgcgt
     7861 tggaactctt cgaacaagaa ggattggcac cttatctgtc gcgctgggaa aagctggata
     7921 attttattaa tcgcccagtg aaacttatca ttggtgataa agaaatattt ggcatttcac
     7981 gcggaataga caaacagggg gctttattac ttgagcagga tggaataata aaaccctgga
     8041 tgggcggtga aatatccctg cgtagtgcag aaaaataatc tagagggccc gcggttcgaa
     8101 caaaaactca tctcagaaga ggatctgaat atgcataccg gtcatcatca ccatcaccat
     8161 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     8221 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     8281 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     8341 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     8401 gtgggctcta tggcttctga ggcgggtgca agtgcaggtg ccagaacatt tctctatcga
     8461 a
//
