LOCUS       sequence-30909-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Karanicolas J, Corn JE, Chen I, Joachimiak LA, Dym O, Peck SH, 
            Albeck S, Unger T, Hu W, Liu G, Delbecq S, Montelione GT, Spiegel 
            CP, Liu DR, Baker D
  TITLE     A de novo protein binding pair by computational design and directed 
            evolution.
  JOURNAL   Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31.
  PUBMED    21458342
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-04-22"; volume: "42(2)"; pages: "250-60. Epub 2011 Mar 
            31"
COMMENT     Sequence Label: Prb(Asp83Asn)
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             496..540
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             562..606
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(644..662)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      658..705
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(829..848)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggtc atcaccacca ccatcacggc
       61 ggcagcaccc gtccgattga tggtctgacg gacgaagata tccgcgagat tctgactcgt
      121 tataaaaaga tcgctctggt gggggcctct ccgaaaccgg aacgcgacgc aaatattgtt
      181 atgaaatatc tgctggagca tggctatgat gtctacccgg taaacccgaa ttatgaagaa
      241 gtgctgggcc gcaagtgcta cccgagtgtt ctggacatcc cggataaaat tgaggtcgta
      301 gatctgtttg tgaacccggc taaagcgtgg cgcttcgttg tctatgccat caagaaaggg
      361 gcaaaagtgg tatggtttca gtacaacaca tattatccgc tggctgcgcg tcaagccaag
      421 gaagcaggcc tgattatcgt tgctaatcgc tgtatgatgc gtgagcacga acgcctgctg
      481 ggcgaaaaag gaggtggcct gaacgatatc ttcgaggccc agaagatcga gtggcacgag
      541 taaggtaccc tcgagtctgg taaagaaacc gctgctgcga aatttgaacg ccagcacatg
      601 gactcgtcta ctagcgcagc ttaattaacc taggctgctg ccaccgctga gcaataacta
      661 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      721 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      781 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      841 tcttcccttc ctttctcgcc acgttcgccg gctttcccgt caagctctaa atcgggggct
      901 c
//
