LOCUS       sequence-309095-       11564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR reporter for mRNA quantification, integrative plasmid into 
            NPR2 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11564)
  AUTHORS   Brion C, Lutz SM, Albert FW
  TITLE     Simultaneous quantification of mRNA and protein in single cells 
            reveals post-transcriptional effects of genetic variation.
  JOURNAL   Elife. 2020 Nov 16;9. pii: 60645. doi: 10.7554/eLife.60645.
  PUBMED    33191917
REFERENCE   2  (bases 1 to 11564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-11-16"; volume: "9. pii: 60645. doi"; pages: " 
            10.7554/eLife.60645"
COMMENT     Sequence Label: Plas-CRISPR_reporter
FEATURES             Location/Qualifiers
     source          1..11564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(8..41)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            complement(104..1460)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(104..301)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(307..1116)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1030..1049
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1117..1460)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    complement(1515..1548)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1600..1704
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1705..1773,1774..2565)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1923..1942)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2736..3324
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3225..3244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3478..3495
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3567..3756)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     3768..3786
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(3818..3967)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(join(4004..5587,5588..5590,5591..8077,8078..8080,
                     8081..8104))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(8114..8134)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8842..9552)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVDGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9120..9139)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9389..9407
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tcacctaata acttcgtata gcatacatta tacgaagtta tattaagggt tctcgagagc
       61 tcgttttcga cactggatgg cggcgttagt atcgaatcga cagcagtata gcgaccagca
      121 ttcacatacg attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga
      181 tgatgtcgag gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta
      241 caatataaaa aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag
      301 tactgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
      361 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
      421 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
      481 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
      541 gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac
      601 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
      661 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
      721 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
      781 aggatattct tctaatacct ggaatgctgt tttgccgggg atcgcagtgg tgagtaacca
      841 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
      901 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
      961 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     1021 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     1081 tcgcggcctc gaaacgtgag tcttttcctt acccatggtt gtttatgttc ggatgtgatg
     1141 tgagaactgt atcctagcaa gattttaaaa ggaagtatat gaaagaagaa cctcagtggc
     1201 aaatcctaac cttttatatt tctctacagg ggcgcggcgt ggggacaatt caacgcgtct
     1261 gtgaggggag cgtttccctg ctcgcaggtc tgcagcgagg agccgtaatt tttgcttcgc
     1321 gccgtgcggc catcaaaatg tatggatgca aatgattata catggggatg tatgggctaa
     1381 atgtacgggc gacagtcaca tcatgcccct gagctgcgca cgtcaagact gtcaaggagg
     1441 gtattctggg cctccatgtc gctggccggg tgacccggcg gggacgaggc aagctaaaca
     1501 gatctctaga cctaataact tcgtatagca tacattatac gaagttatat taagggttgt
     1561 cgacgctcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1621 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1681 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1741 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1801 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1861 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1921 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1981 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2041 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2101 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2161 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2221 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2281 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2341 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2401 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2461 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2521 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2581 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2641 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2701 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2761 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2821 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2881 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2941 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3001 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3061 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3121 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3181 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3241 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3301 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3361 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3421 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3481 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3541 tggccgattc attaatgcag ctggatcttc gagcgtccca aaaccttctc aagcaaggtt
     3601 ttcagtataa tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat
     3661 ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca
     3721 ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg gggaagggcg tgaatgtaag
     3781 cgtgacataa ctaattacat gactcgagtt cctaggaaag catgtctaag tcgaaatcgt
     3841 ccagagcgtc tgatccaagc atatccaggt caaaatcatc taaggcgtca cttccaagca
     3901 tgtcgaggtc aaagtcatct agcgcatcag aacccagcat atccaagtcg aagtcgtcca
     3961 aagcgtcaga acctccaccg tcgactccag atctaccaga accatcacca cctaattgag
     4021 acaagtcgat tctagtttcg tacaaaccag taatagattg gtggatcaaa gtagcgtcca
     4081 aaacttcttt ggtagaggtg tatctctttc tgtcgatagt ggtatcgaaa tacttaaaag
     4141 cagctggagc acccaagtta gtcaaagtga acaagtgaat gatgttttca gcttgttctc
     4201 taattggctt atctctgtgc ttgttgtaag ctgacaaaac tttgtccaaa ttggcgtcag
     4261 ccaaaatgac tcttttggaa aattcggaaa tttgttcaat gatttcgtcc aagtagtgct
     4321 tgtgttgttc gacaaataat tgcttttgtt cgttatcttc aggagaacct ttcaattttt
     4381 cgtagtggga ggccaagtat aagaaattaa cgtacttgga tggcaaagcc aattcattac
     4441 ccttttgtaa ttcaccggca gaagccaaca ttctctttct accattttcc aattcgaaca
     4501 aactgtactt tggcaactta atgatcaagt ccttcttgac ttctttgtaa cccttggctt
     4561 ccaaaaagtc gataggattc ttttcgaatg aggatctttc catgatagtg atacctaata
     4621 attctttgac ggacttcaac ttcttactct tacccttttc gaccttagca acaaccaaaa
     4681 cagagtaagc aacagttgga gaatcaaaac caccgtactt ttttgggtcc cagtcttttt
     4741 ttctagcgat caacttatcg gagtttctct taggcaaaat ggattctttg gagaaaccac
     4801 cagtttgaac ttcggtcttt ttgacgatgt ttacttgtgg catggacaat acctttctaa
     4861 cagtggcaaa atctctaccc ttatcccaaa cgatttcacc tgtttcacca ttagtttcaa
     4921 tcaatggtct ttttctaatt tcaccgttgg ccaaagtgat ttcggtctta aagaaattca
     4981 taatattgga gtagaagaag tatttggcag tagctttacc aatttcttgt tcggacttgg
     5041 cgatcatctt tctaacgtcg taaaccttgt aatcaccgta gacaaattcg gattccaact
     5101 ttggatactt cttaatcaag gcagtaccaa caacagcatt caagtaagca tcatgtgcat
     5161 gatggtagtt gttgatttct ctgaccttgt agaattggaa gtcctttctg aaatcggaaa
     5221 ccaacttgga cttcaaggta atgaccttaa cttctctgat caatttgtcg ttttcgtcgt
     5281 acttagtgtt cattctacta tccaaaattt gagcaacgtg cttggtgatt tgtctggttt
     5341 ctactaattg tcttttgatg aaacctgcct tatccaattc agacaaacca cctctttcag
     5401 ccttggtcaa attgtcaaac tttctttggg tgatcaactt ggcgttcaat aattgtctcc
     5461 aatagttctt cattttctta acgacttctt cagatggaac gttatcggac ttacctctat
     5521 tcttatcgga tctagtcaag accttgttgt caatggagtc atccttcaaa aaagattgtg
     5581 gaacgatagc gtcaacatcg taatcagaca atctgttgat gtccaattct tggtcaacgt
     5641 acatatctct accgttttgc aagtagtaca agtacaactt ttcgttttgt aattgggtgt
     5701 tttcaactgg gtgttctttc aaaatttggg aacccaattc tttgatacct tcttcgattc
     5761 tcttcattct ttctctggag ttcttttgac ccttttgggt ggtttgattt tctctagcca
     5821 tttcgataac gatgttttct ggcttatgtc tacccataac tttgaccaat tcgtcaacga
     5881 ccttaacggt ttgtaaaata cccttcttaa tagctggaga accagccaag ttagcgatat
     5941 gttcatgtaa agaatcacct tgaccagaga cttgggcctt ttgtatatct tctttgaaag
     6001 tcaaggaatc gtcgtggatt aattgcatga agtttctgtt agcgaaacca tcagacttca
     6061 aaaagtccaa aatggtctta ccggattgct tgtctctgat accgttaatc aattttctgg
     6121 acaatctacc ccaaccagtg tatcttcttc tctttaattg cttcataacc ttatcatcga
     6181 acaagtgggc gtaggttttc aatctttctt caatcatttc tctatcttca aacaaggtca
     6241 atgtcaagac aatatcttcc aaaatatctt cgttttcttc gttgtccaag aaatccttgt
     6301 ccttgatgat tttcaacaag tcatggtaag tacctaaaga ggcattgaat ctatcttcga
     6361 caccagaaat ttcaacggag tcgaagcatt cgattttctt gaagtaatct tcttttaatt
     6421 gcttgacagt aacctttctg ttggtcttga acaacaaatc gacgatagcc ttcttttgtt
     6481 caccggataa aaaagctggt tttctcatac cttcggtgac gtacttaacc ttagtcaatt
     6541 cgttgtagac ggtgaagtat tcgtacaaca aagagtgctt tggcaaaacc ttttcgtttg
     6601 gcaagttctt gtcgaagttg gtcattcttt caataaagga ttgggcagaa gcacccttat
     6661 ctacaacttc ttcgaagttc caaggagtaa tagtttcttc ggactttcta gtcatccagg
     6721 caaatcttga attacctcta gccaatggac caacgtagta agggattctg aaggtcagaa
     6781 tcttttcgat cttttctctg ttgtccttca agaatggata aaaatcttct tgtcttctca
     6841 aaattgcgtg caattcaccc aagtgaattt gatgtggaat agaaccgttg tcgaaggttc
     6901 tttgctttct caacaaatct tctctgttca acttgaccaa taattcttca gtaccgtcca
     6961 tcttttccaa gattggcttg atgaacttgt aaaattcttc ttgagaagca ccaccatcaa
     7021 tataaccggc gtaaccgttc ttggattgat cgaagaaaat ttctttgtac ttttcaggta
     7081 attgttgtct gaccaaagcc ttcaacaaag tcaagtcttg gtggtgttca tcgtatcttt
     7141 tgatcataga ggcagacaat ggagccttag taatttcggt gttgactctc aaaatatcgg
     7201 acaacaaaat ggcatcggac aagttcttag cagccaaaaa caaatcagcg tattgatcac
     7261 cgatttgagc caacaagtta tccaaatcgt catcgtaggt atccttagat aattgcaact
     7321 tggcatcttc agccaaatcg aaattggact tgaagtttgg agtcaaaccc aaagacaagg
     7381 caatcaaatt accgaacaaa ccattcttct tttcacctgg taattgggcg atcaaatttt
     7441 ctaatcttct ggacttggac aatctggcag acaaaatagc tttagcatca acaccagagg
     7501 cgttgattgg gttttcttcg aataattgat tgtaggtttg gactaattgg atgaacaact
     7561 tatcaacatc ggagttatct gggttcaaat caccttcgat caagaaatga cctctgaact
     7621 taatcatgtg ggccaaagcc aaatagatca atctcaaatc agccttatcg gtagagtcaa
     7681 ccaacttctt tctcaaatgg tagatagttg ggtacttttc gtggtaagca acttcatcaa
     7741 cgatgttacc gaagattgga tgtctttcgt gcttcttatc ttcttcgacc aagaaggatt
     7801 cttccaatct gtggaagaat gaatcatcaa ccttggccat ttcgttggag aagatttctt
     7861 gcaagtagca gattctgttc tttcttctgg tgtatcttct tctagcggtt cttttcaatc
     7921 tagtagcttc agcagtttca ccagaatcga acaacaaggc accaatcaag ttctttttga
     7981 tggagtgtct atcagtgtta cccaaaacct tgaacttctt ggatggaacc ttgtattcat
     8041 cagtaataac agcccaacca acagagtttg taccgatagc caaaccaata gagtatttct
     8101 tatccccggg tctaactttt ctcttcttct ttggggctct agacattctt ttaatcgtgg
     8161 atcctgttaa ttcagtaaat tttcgatctt gggaagaaaa aagcagtaag cgtgaaaaat
     8221 ctaaaagctg atgtagtaga agatcctatt ctttaacaaa gattgacctt ttctttttct
     8281 tcttggtttg agtagaaagg ggaaggaaga atacaagaga gaggaaaaaa aggaagataa
     8341 aaagagagcg tgatataaat gaatatatat taaacaagag agattgggaa ggaaaggatc
     8401 aaacaaaccc aaaaatattt caaaaaggag agagagaggc gagtttggtt tcaaaacggt
     8461 ttatttattt atgcaagagg acgtggaaga aaaagaagaa ggaagaaaaa aatttgaaag
     8521 aaaaaaacgc gtggcgggta aagaagaaaa tggaaaatag aggccgggtg acagagaaat
     8581 attgagggtt aattggaaaa tatgttaggg tgaggcatat gtttttaagg gttttgagga
     8641 tccgataagg aagaatgtag gttaaatgtt gtgcattaat tgctgtggca gcttacccgc
     8701 ttccccacac attttggtag tatctgtcct cttgttattg ttactgtaat tgtgtatata
     8761 tgttctcgcg tgtgtcttat ttacttattt agttattata ttatatgggt ctgcaaggta
     8821 gaggcggcgc gtggcggccg ctcacttgta cagctcgtcc atgccgccgg tggagtggcg
     8881 gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc
     8941 caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt
     9001 ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct gcttgatctc
     9061 gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatagt
     9121 cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta
     9181 gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc
     9241 gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg
     9301 gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat
     9361 gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc
     9421 ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt ccacggagcc
     9481 ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat cctcctcgcc
     9541 cttgctcacc atcattttaa tatctagatt agtgtgtgta tttgtgtttg tgtgtctata
     9601 gaagtatagt aatttatgct gcaaaggtcc taatgtataa ggaaagaata tttagagaaa
     9661 agaagaaaac aagagtttta tatacataca gagcacatgc atgccatagg atcctactgt
     9721 atgtacatac agtacccggg ctgcaggaat tcgatggtcc aaccatgatg agtatagtcc
     9781 cttacattga tggcttaaac agcattgcca aaatttctaa actaagcaac agtgatcctg
     9841 gcctagtgat agagtgcata cgtcacttaa tatactataa gtgtgttaca ttatcagata
     9901 ttttccaatt ttctaacata tacgctcctt cttcattaat taggaatttt ttgactgatc
     9961 cactaatggc tagtgactgt caatcttatg ttacgttccc agaagtgtct aaaatatcga
    10021 acttgccctt aaacaaaagt ctgggttcag gtgaccaaga ctcaccatca ttttctgtac
    10081 gaagaaaatc caaatcatct agcataccgt caaacccgga ttctagaact acatctttta
    10141 gttctaccag tagagtttct caaaactcct ccttaaattc ctctttttca tcaatttata
    10201 aagactggag gcaatcacaa acctcctgct cgagttcaaa tattcatgtc atcaacaatc
    10261 gtaaccgatt cctaccaaca agatcatgcc tatttgacct ttataggtca ctttcacaag
    10321 gtcaaacgct gaaaacatgg tatgagtcga aatatatgat tttaaaagaa aataacattg
    10381 atataagaag gttcataacg tttggcctgg aaaaacgtat catttacagg tgctactctt
    10441 ttcctgtaat gataaatgca ggttcacgtg aaccaaaaga aatgacacca ataataacaa
    10501 aagatttagt gaataatgat aagttacttg aaaaaagaaa tcacaatcat ttgctttcag
    10561 ccacagggtc tagaaatacg gcacaatccg gcaacttgaa accagaaaga ccatcaaagg
    10621 tttcatttga aatgcaaagg gtaagttcgt tggcaacagg caaaagtact atgccgaaac
    10681 tcagcgacga agaagaaggt attcttgaag agtctattag gaatgccgaa acatttgata
    10741 aaatttgcgt tttactgagc aagcctaaat tagaagtcga aagctaccta aacgaattag
    10801 gagaatttaa atgctaagct atttccaagg gtttgtgccc atacacacaa tattttactc
    10861 cgtgttccat cctacagaag gttctaagat taaatacgag tttccgccaa ataatttgaa
    10921 aaatcatggt attaatttca acacatttaa aaactacatc ataccgaagc ctatattatg
    10981 ccacaagttg atcactttta agtatggtac atataggatt gtatgttatc ctgtgacaat
    11041 caattctcca atatatgcta ggaatttttt tagttttaat tttgtatttg tattcccata
    11101 tgattgtgag acgtctccct acgaaccagc tattactagg ctgggcaaaa tgttcaaagt
    11161 tcttgaagaa caaaatcaat tgctatccaa atcggaaaga gatccagttt tcttcgattt
    11221 gaaggtgttg gaaaactcca caacaacacc ctccactgca ggtccttcat ccacgccaaa
    11281 tcctagtagt aacaccacac caactcatcc tacatccgaa aaggatacaa aggatatgag
    11341 aagcagcaga tacagtgacc ttatcaagga tttgggcctt ccgcaatccg cattttccat
    11401 acaagattta ttgatgagaa ttttccaaga cctgaataac tattccgaat gcctcatacc
    11461 aattgatgag ggaaatgcag tagacataaa gatttttccg ctgttgagac caccgacaac
    11521 ttgtgtctca ttagaagatg tgcccctgtc gtctgtaaat ttga
//
