LOCUS       sequence-309118-       10557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SYK in insect cell (sf9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Lee SJ, Choi JS, Bong SM, Hwang HJ, Lee J, Song HJ, Lee J, Kim JH, 
            Koh JS, Lee BI
  TITLE     Crystal Structures of Spleen Tyrosine Kinase in Complex with Two 
            Novel 4-Aminopyrido[4,3-d] Pyrimidine Derivative Inhibitors.
  JOURNAL   Mol Cells. 2018 Jun;41(6):545-552. doi: 10.14348/molcells.2018.2219.
            Epub 2018 Jun 12.
  PUBMED    29890824
REFERENCE   2  (bases 1 to 10557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.14348/molcells.2018.2219"; journalName: "Mol Cells"; date: 
            "2018-06"; volume: "41"; issue: "6"; pages: "545-552"
COMMENT     Sequence Label: pVL1393-MAL-SYK
FEATURES             Location/Qualifiers
     source          1..10557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             990..1013
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_recomb     1565..2270
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           1653..2025
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(1682..2270)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(2704..2721)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3938..3954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3938..3954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3951..3973)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3962..3978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3986..3992,3993..4010,4011..4016))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4031..4052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4169..4186)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4340..4928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4420..4439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5099..5890,5891..5959))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5722..5741
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5960..6064)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6132..6150
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6188..6210)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6310..6329
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6523..6545
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6537..6554
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6538..6554
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     9727..10554
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             9727..9851
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(9889..10494)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atccatggct ctggaggaga tcaggcccaa ggaggtttac ctggaccgaa
      181 agctgctgac gctggaagac aaagaactgg gctctggtaa ttttggaact gtgaaaaagg
      241 gctactacca aatgaaaaaa gttgtgaaaa ccgtggctgt gaaaatactg aaaaacgagg
      301 ccaatgaccc cgctcttaaa gatgagttat tagcagaagc aaatgtcatg cagcagctgg
      361 acaacccgta catcgtgcgc atgatcggga tatgcgaggc cgagtcctgg atgctagtta
      421 tggagatggc agaacttggt cccctcaata agtatttgca gcagaacaga catgtcaagg
      481 ataagaacat catagaactg gttcatcagg tttccatggg catgaagtac ttggaggaga
      541 gcaattttgt gcacagagat ctggctgcaa gaaatgtgtt gctagttacc caacattatg
      601 ccaagatcag tgatttcgga ctctccaaag cactgcgtgc tgatgaaaac tactacaagg
      661 cccagaccca tggaaagtgg cctgtcaagt ggtacgctcc ggaatgcatc aactactaca
      721 agttctccag caaaagcgat gtctggagct ttggagtgtt gatgtgggaa gcattctcct
      781 atgggcagaa gccatatcga gggatgaaag gaagtgaagt caccgctatg ttagagaaag
      841 gagagcggat ggggtgccct gcagggtgtc caagagagat gtacgatctc atgaatctgt
      901 gctggacata cgatgtggaa aacaggcccg gattcgcagc agtggaactg cggctgcgca
      961 attactacta tgacgtggtg aacctcgagc accaccacca ccaccaccac cactaattga
     1021 ttaataccta ggctgctaaa caaagcccga aaggaagcga attccggagc ggccgctgca
     1081 gatctgatcc tttcctggga cccggcaaga accaaaaact cactctcttc aaggaaatcc
     1141 gtaatgttaa acccgacacg atgaagcttg tcgttggatg gaaaggaaaa gagttctaca
     1201 gggaaacttg gacccgcttc atggaagaca gcttccccat tgttaacgac caagaagtga
     1261 tggatgtttt ccttgttgtc aacatgcgtc ccactagacc caaccgttgt tacaaattcc
     1321 tggcccaaca cgctctgcgt tgcgaccccg actatgtacc tcatgacgtg attaggatcg
     1381 tcgagccttc atgggtgggc agcaacaacg agtaccgcat cagcctggct aagaagggcg
     1441 gcggctgccc aataatgaac cttcactctg agtacaccaa ctcgttcgaa cagttcatcg
     1501 atcgtgtcat ctgggagaac ttctacaagc ccatcgttta catcggtacc gactctgctg
     1561 aagaggagga aattctcctt gaagtttccc tggtgttcaa agtaaaggag tttgcaccag
     1621 acgcacctct gttcactggt ccggcgtatt aaaacacgat acattgttat tagtacattt
     1681 attaagcgct agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata
     1741 attcattaaa tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag
     1801 cgtctttata tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat
     1861 tagtttcaaa caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc
     1921 tcaacgccac aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt
     1981 gtgttttgtt ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc
     2041 tttcatcact gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat aaagcttgga
     2101 catatttaac atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct
     2161 cgtcgttaga agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg
     2221 tgtcggctaa cacgtccgcg atcaaatttg tagttgagct ttttggaatt atttctgatt
     2281 gcgggcgttt ttgggcgggt ttcaatctaa ctgtgcccga ttttaattca gacaacacgt
     2341 tagaaagcga tggtgcaggc ggtggtaaca tttcagacgg caaatctact aatggcggcg
     2401 gtggtggagc tgatgataaa tctaccatcg gtggaggcgc aggcggggct ggcggcggag
     2461 gcggaggcgg aggtggtggc ggtgatgcag acggcggttt aggctcaaat gtctctttag
     2521 gcaacacagt cggcacctca actattgtac tggtttcggg cgccgttttt ggtttgaccg
     2581 gtctgagacg agtgcgattt ttttcgtttc taatagcttc caacaattgt tgtctgtcgt
     2641 ctaaaggtgc agcgggttga ggttccgtcg gcattggtgg agcgggcggc aattcagaca
     2701 tcgatggtgg tggtggtggt ggaggcgctg gaatgttagg cacgggagaa ggtggtggcg
     2761 gcggtgccgc cggtataatt tgttctggtt tagtttgttc gcgcacgatt gtgggcaccg
     2821 gcgcaggcgc cgctggctgc acaacggaag gtcgtctgct tcgaggcagc gcttggggtg
     2881 gtggcaattc aatattataa ttggaataca aatcgtaaaa atctgctata agcattgtaa
     2941 tttcgctatc gtttaccgtg ccgatattta acaaccgctc aatgtaagca attgtattgt
     3001 aaagagattg tctcaagctc gcacgccgat aacaagcctt ttcattttta ctacagcatt
     3061 gtagtggcga gacacttcgc tgtcgtcgac gtacatgtat gctttgttgt caaaaacgtc
     3121 gttggcaagc tttaaaatat ttaaaagaac atctctgttc agcaccactg tgttgtcgta
     3181 aatgttgttt ttgataattt gcgcttccgc agtatcgaca cgttcaaaaa attgatgcgc
     3241 atcaattttg ttgttcctat tattgaataa ataagattgt acagattcat atctacgatt
     3301 cgtcatggcc accacaaatg ctacgctgca aacgctggta caattttacg aaaactgcaa
     3361 aaacgtcaaa actcggtata aaataatcaa cgggcgcttt ggcaaaatat ctattttatc
     3421 gcacaagccc actagcaaat tgtatttgca gaaaacaatt tcggcgcaca attttaacgc
     3481 tgacgaaata aaagttcacc agttaatgag cgaccaccca aattttataa aaatctattt
     3541 taatcacggt tccatcaaca accaagtgat cgtgatggac tacattgact gtcccgattt
     3601 atttgaaaca ctacaaatta aaggcgagct ttcgtaccaa cttgttagca atattattag
     3661 acagctgtgt gaagcgctca acgatttgca caagcacaat ttcatacaca acgacataaa
     3721 actcgaaaat gtcttatatt tcgaagcact tgatcgcgtg tatgtttgcg attacggatt
     3781 gtgcaaacac gaaaactcac ttagcgtgca cgacggcacg ttggagtatt ttagtccgga
     3841 aaaaattcga caccacaact atgcacgttc gtttgactgg tacgccgtcg gcgtgttaac
     3901 atacaagttg ctaaccggcg gccaattcgt aatcatggtc atagctgttt cctgtgtgaa
     3961 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4021 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     4081 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4141 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4201 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4261 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4321 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4381 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4441 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4501 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4561 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4621 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4681 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4741 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4801 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4861 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4921 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4981 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5041 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5101 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5161 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5221 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5281 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5341 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5401 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5461 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5521 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5581 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5641 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5701 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5761 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5821 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5881 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5941 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6001 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6061 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     6121 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     6181 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     6241 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     6301 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     6361 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     6421 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     6481 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     6541 aaacgacggc cagtgccaag cttggctcgt aaagcgagtt gaaggatcat atttagttgc
     6601 gtttatgaga taagattgaa agcacgtgta aaatgtttcc cgcgcgttgg cacaactatt
     6661 tacaatgcgg ccaagttata aaagattcta atctgatatg ttttaaaaca cctttgcggc
     6721 ccgagttgtt tgcgtacgtg actagcgaag aagatatgtg gaccgcagaa cagatagtaa
     6781 aacaaaaccc tagtattgga gcaataatcg atttaaccaa cacgtctaaa tattatgatg
     6841 gtgtgcattt tttgcgggcg ggcctgttat acaaaaaaat tcaagtacct ggccagactt
     6901 tgccgcctga aagcatagtt caagaattta ttgacacggt aaaagaattt acagaaaagt
     6961 gtcccggcat gttggtgggc gtgcactgca cacacggtat taatcgcacc ggttacatgg
     7021 tgtgcagata tttaatgcac accctgggta ttgcgccgca ggaagccata gatagattcg
     7081 aaaaagccag aggtcacaaa attgaaagac aaaattacgt tcaagattta ttaatttaat
     7141 taatattatt tgcattcttt aacaaatact ttatcctatt ttcaaattgt tgcgcttctt
     7201 ccagcgaacc aaaactatgc ttcgcttgct ccgtttagct tgtagccgat cagtggcgtt
     7261 gttccaatcg acggtaggat taggccggat attctccacc acaatgttgg caacgttgat
     7321 gttacgttta tgcttttggt tttccacgta cgtcttttgg ccggtaatag ccgtaaacgt
     7381 agtgccgtcg cgcgtcacgc acaacaccgg atgtttgcgc ttgtccgcgg ggtattgaac
     7441 cgcgcgatcc gacaaatcca ccactttggc aactaaatcg gtgacctgcg cgtctttttt
     7501 ctgcattatt tcgtctttct tttgcatggt ttcctggaag ccggtgtaca tgcggtttag
     7561 atcagtcatg acgcgcgtga cctgcaaatc tttggcctcg atctgcttgt ccttgatggc
     7621 aacgatgcgt tcaataaact cttgtttttt aacaagttcc tcggtttttt gcgccaccac
     7681 cgcttgcagc gcgtttgtgt gctcggtgaa tgtcgcaatc agcttagtca ccaactgttt
     7741 gctctcctcc tcccgttgtt tgatcgcggg atcgtacttg ccggtgcaga gcacttgagg
     7801 aattacttct tctaaaagcc attcttgtaa ttctatggcg taaggcaatt tggacttcat
     7861 aatcagctga atcacgccgg atttagtaat gagcactgta tgcggctgca aatacagcgg
     7921 gtcgcccctt ttcacgacgc tgttagaggt agggccccca ttttggatgg tctgctcaaa
     7981 taacgatttg tatttattgt ctacatgaac acgtatagct ttatcacaaa ctgtatattt
     8041 taaactgtta gcgacgtcct tggccacgaa ccggacctgt tggtcgcgct ctagcacgta
     8101 ccgcaggttg aacgtatctt ctccaaattt aaattctcca attttaacgc gagccatttt
     8161 gatacacgtg tgtcgatttt gcaacaacta ttgtttttta acgcaaacta aacttattgt
     8221 ggtaagcaat aattaaatat gggggaacat gcgccgctac aacactcgtc gttatgaacg
     8281 cagacggcgc cggtctcggc gcaagcggct aaaacgtgtt gcgcgttcaa cgcggcaaac
     8341 atcgcaaaag ccaatagtac agttttgatt tgcatattaa cggcgatttt ttaaattatc
     8401 ttatttaata aatagttatg acgcctacaa ctccccgccc gcgttgactc gctgcacctc
     8461 gagcagttcg ttgacgcctt cctccgtgtg gccgaacacg tcgagcgggt ggtcgatgac
     8521 cagcggcgtg ccgcacgcga cgcacaagta tctgtacacc gaatgatcgt cgggcgaagg
     8581 cacgtcggcc tccaagtggc aatattggca aattcgaaaa tatatacagt tgggttgttt
     8641 gcgcatatct atcgtggcgt tgggcatgta cgtccgaacg ttgatttgca tgcaagccga
     8701 aattaaatca ttgcgattag tgcgattaaa acgttgtaca tcctcgcttt taatcatgcc
     8761 gtcgattaaa tcgcgcaatc gagtcaagtg atcaaagtgt ggaataatgt tttctttgta
     8821 ttcccgagtc aagcgcagcg cgtattttaa caaactagcc atcttgtaag ttagtttcat
     8881 ttaatgcaac tttatccaat aatatattat gtatcgcacg tcaagaatta acaatgcgcc
     8941 cgttgtcgca tctcaacacg actatgatag agatcaaata aagcgcgaat taaatagctt
     9001 gcgacgcaac gtgcacgatc tgtgcacgcg ttccggcacg agctttgatt gtaataagtt
     9061 tttacgaagc gatgacatga cccccgtagt gacaacgatc acgcccaaaa gaactgccga
     9121 ctacaaaatt accgagtatg tcggtgacgt taaaactatt aagccatcca atcgaccgtt
     9181 agtcgaatca ggaccgctgg tgcgagaagc cgcgaagtat ggcgaatgca tcgtataacg
     9241 tgtggagtcc gctcattaga gcgtcatgtt tagacaagaa agctacatat ttaattgatc
     9301 ccgatgattt tattgataaa ttgaccctaa ctccatacac ggtattctac aatggcgggg
     9361 ttttggtcaa aatttccgga ctgcgattgt acatgctgtt aacggctccg cccactatta
     9421 atgaaattaa aaattccaat tttaaaaaac gcagcaagag aaacatttgt atgaaagaat
     9481 gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa caagattaat atgcctccgt
     9541 gtataaaaaa aatattgaac gatttgaaag aaaacaatgt accgcgcggc ggtatgtaca
     9601 ggaagaggtt tatactaaac tgttacattg caaacgtggt ttcgtgtgcc aagtgtgaaa
     9661 accgatgttt aatcaaggct ctgacgcatt tctacaacca cgactccaag tgtgtgggtg
     9721 aagtcatgca tcttttaatc aaatcccaag atgtgtataa accaccaaac tgccaaaaaa
     9781 tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg caagggtctc aatcctattt
     9841 gtaattattg aataataaaa caattataaa tgtcaaattt gttttttatt aacgatacaa
     9901 accaaacgca acaagaacat ttgtagtatt atctataatt gaaaacgcgt agttataatc
     9961 gctgaggtaa tatttaaaat cattttcaaa tgattcacag ttaatttgcg acaatataat
    10021 tttattttca cataaactag acgccttgtc gtcttcttct tcgtattcct tctctttttc
    10081 atttttctcc tcataaaaat taacatagtt attatcgtat ccatatatgt atctatcgta
    10141 tagagtaaat tttttgttgt cataaatata tatgtctttt ttaatggggt gtatagtacc
    10201 gctgcgcata gtttttctgt aatttacaac agtgctattt tctggtagtt cttcggagtg
    10261 tgttgcttta attattaaat ttatataatc aatgaatttg ggatcgtcgg ttttgtacaa
    10321 tatgttgccg gcatagtacg cagcttcttc tagttcaatt acaccatttt ttagcagcac
    10381 cggattaaca taactttcca aaatgttgta cgaaccgtta aacaaaaaca gttcacctcc
    10441 cttttctata ctattgtctg cgagcagttg tttgttgtta aaaataacag ccattgtaat
    10501 gagacgcaca aactaatatc acaaactgga aatgtctatc aatatatagt tgctgat
//
