LOCUS       sequence-309119-        5271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine RGS4 protein with HA-tag at C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Bray ER, Yungher BJ, Levay K, Ribeiro M, Dvoryanchikov G, Ayupe AC, 
            Thakor K, Marks V, Randolph M, Danzi MC, Schmidt TM, Chaudhari N, 
            Lemmon VP, Hattar S, Park KK
  TITLE     Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion 
            Cells.
  JOURNAL   Neuron. 2019 Aug 21;103(4):642-657.e7. doi: 
            10.1016/j.neuron.2019.05.044. Epub 2019 Jun 26.
  PUBMED    31255486
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2019.05.044"; journalName: "Neuron"; date: 
            "2019-08-21- 21"; volume: "103"; issue: "4"; pages: "642-657.e7"
COMMENT     Sequence Label: pAAV.CMV.RGS4.C-HA
FEATURES             Location/Qualifiers
     source          1..5271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        279..482
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     433..453
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     479..503
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          631..1009
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             1739..1765
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1776..2252
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1975..1994)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2292..2432
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2292..2421
                     /label=AAV2 ITR
     rep_origin      2507..2962
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2594..2613)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2804..2825
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2979..2998)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3098..3120
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3158..3176)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3244..3348
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3349..3417,3418..4209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3567..3586)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4380..4968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4869..4888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5030..5170
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5041..5170
                     /label=AAV2 ITR
     enhancer        join(5246..5271,1..278)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
       61 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      121 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      181 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      241 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      301 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      361 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      421 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      481 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      541 gaagacaccg ggaccgatcc agcctccgcg gattcgaatc ccggccggga acggtgcatt
      601 ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg cctatagagt ctataggccc
      661 acaaaaaatg ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc
      721 ctaatctctt tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct
      781 aaagaataac agtgataatt tctgggttaa ggcaatagca atatttctgc atataaatat
      841 ttctgcatat aaattgtaac tgatgtaaga ggtttcatat tgctaatagc agctacaatc
      901 cagctaccat tctgctttta ttttatggtt gggataaggc tggattattc tgagtccaag
      961 ctaggccctt ttgctaatca tgttcatacc tcttatcttc ctcccacagc tcctgggcaa
     1021 cgtgctggtc tgtgtgctgg cccatcactt tggcaaagaa ttgggattcg aacatcgatt
     1081 gaattccccg gggatcctct agagtcgacc tgcagaagct atgtgcaaag gacttgcagg
     1141 tctgccggct tcctgcctga ggagtgcaaa ggacatgaaa catcggctgg ggttcctgct
     1201 gcagaagtca gattcctgcg aacacagttc ttcacacagc aagaaggaca aagtagtaac
     1261 ttgccagagg gtaagccaag aagaagtcaa gaaatgggct gaatcgttgg aaaacctgat
     1321 tcaccatgaa tgtggactgg cagctttcaa agctttcctg aagtcggaat acagcgagga
     1381 gaacattgac ttctggatca gctgtgagga gtacaagaag atcaagtcac cttctaaact
     1441 aagtcccaaa gccaagaaga tctacaacga gttcatctca gtgcaagcaa caaaagaggt
     1501 gaacctggac tcttgcacca gagaggagac aagccggaac atgttacagc ccacaataac
     1561 ctgttttgat gaagcccaga agaagatttt caacctgatg gaaaaggatt cctaccgccg
     1621 cttcctcaag tctcgattct atcttgacct gaccaatcct tccagctgcg gggcagagaa
     1681 gcagaaagga gccaagagtt ctgcagactg cacttccctg gtctcccagt gtgcagctta
     1741 cccatacgat gttccagatt acgcttagga tctacgggtg gcatccctgt gacccctccc
     1801 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
     1861 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
     1921 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt
     1981 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
     2041 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
     2101 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
     2161 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
     2221 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac
     2281 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     2341 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     2401 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     2461 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     2521 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     2581 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     2641 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     2701 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     2761 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     2821 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
     2881 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     2941 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     3001 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3061 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     3121 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     3181 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     3241 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3301 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3361 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3421 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3481 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3541 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3601 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3661 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3721 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3781 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3841 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3901 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3961 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4021 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4081 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4141 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4201 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4261 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4321 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4381 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4441 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4501 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4561 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4621 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4681 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4741 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4801 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4861 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4921 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4981 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtc ctgcaggcag
     5041 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
     5101 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
     5161 aggggttcct gcggccgcac gcgtctagtt attaatagta atcaattacg gggtcattag
     5221 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc c
//
