LOCUS       sequence-30912-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Karanicolas J, Corn JE, Chen I, Joachimiak LA, Dym O, Peck SH, 
            Albeck S, Unger T, Hu W, Liu G, Delbecq S, Montelione GT, Spiegel 
            CP, Liu DR, Baker D
  TITLE     A de novo protein binding pair by computational design and directed 
            evolution.
  JOURNAL   Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31.
  PUBMED    21458342
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-04-22"; volume: "42(2)"; pages: "250-60. Epub 2011 Mar 
            31"
COMMENT     Sequence Label: sankE3_5
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             527..544
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(597..615)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      611..658
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(782..801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggggaattcc ctctgaatat tttgtttact ttaagaagga gatatacata tgggttctga
       61 cctgggtaaa aaactgctgg aagcagcgcg tgcgggtcag gacgacgaag ttcgtatcct
      121 gatggcgaat ggtgcggatg ttaacgcaac cgacaacgac ggttacacgc ctctccatct
      181 ggcggcctct aacggtcatc tggaaatcgt ggaagtcctg ctgaaaaacg gtgcagacgt
      241 aaatgcgtcc gacctgaccg gtatcacccc actgcatctc gcggcagcaa ccggccacct
      301 ggagatcgtt gaagttctcc tgaaacacgg tgctgatgta aacgcgtacg ataatgacgg
      361 ccacaccccg ctgcacctcg ccgcgaaata cggtcacctc gaaattgttg aggttctgct
      421 caagcatggc gcggacgtga acgcgcagga caaattcggt aaaaccgcgt tcgacatctc
      481 tatcgacaat ggtaacgaag acctcgcgga aatcctgcag ctcgagcacc accaccacca
      541 ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      601 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      661 gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc
      721 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      781 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
      841 ctaaat
//
