LOCUS       sequence-309120-        5590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine CD86 protein (T-lymphocyte activation antigen) with
            HA-tag at C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Bray ER, Yungher BJ, Levay K, Ribeiro M, Dvoryanchikov G, Ayupe AC, 
            Thakor K, Marks V, Randolph M, Danzi MC, Schmidt TM, Chaudhari N, 
            Lemmon VP, Hattar S, Park KK
  TITLE     Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion 
            Cells.
  JOURNAL   Neuron. 2019 Aug 21;103(4):642-657.e7. doi: 
            10.1016/j.neuron.2019.05.044. Epub 2019 Jun 26.
  PUBMED    31255486
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2019.05.044"; journalName: "Neuron"; date: 
            "2019-08-21- 21"; volume: "103"; issue: "4"; pages: "642-657.e7"
COMMENT     Sequence Label: pAAV.CMV.CD86.C-HA
FEATURES             Location/Qualifiers
     source          1..5590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     196..215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   357..497
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   368..497
                     /label=AAV2 ITR
     enhancer        573..876
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        877..1080
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1031..1051
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1077..1101
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1229..1607
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             2656..2682
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2693..3169
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2892..2911)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3209..3349
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3209..3338
                     /label=AAV2 ITR
     rep_origin      3424..3879
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3511..3530)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3721..3742
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3896..3915)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4015..4037
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4075..4093)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4161..4265
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4266..4334,4335..5126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4484..4503)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5297..5590,1..295)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
       61 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      121 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      181 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      241 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      301 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtcctg
      361 caggcagctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc
      421 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
      481 catcactagg ggttcctgcg gccgcacgcg tctagttatt aatagtaatc aattacgggg
      541 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      601 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      661 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      721 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      781 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      841 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      901 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      961 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     1021 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     1081 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     1141 agacaccggg accgatccag cctccgcgga ttcgaatccc ggccgggaac ggtgcattgg
     1201 aacgcggatt ccccgtgcca agagtgacgt aagtaccgcc tatagagtct ataggcccac
     1261 aaaaaatgct ttcttctttt aatatacttt tttgtttatc ttatttctaa tactttccct
     1321 aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc accattctaa
     1381 agaataacag tgataatttc tgggttaagg caatagcaat atttctgcat ataaatattt
     1441 ctgcatataa attgtaactg atgtaagagg tttcatattg ctaatagcag ctacaatcca
     1501 gctaccattc tgcttttatt ttatggttgg gataaggctg gattattctg agtccaagct
     1561 aggccctttt gctaatcatg ttcatacctc ttatcttcct cccacagctc ctgggcaacg
     1621 tgctggtctg tgtgctggcc catcactttg gcaaagaatt gggattcgaa catcgattga
     1681 attccccggg gatcctctag agtcgacctg cagaagctac ccacgatgga ccccagatgc
     1741 accatgggct tggcaatcct tatctttgtg acagtcttgc tgatctcaga tgctgtttcc
     1801 gtggagacgc aagcttattt caatgggact gcatatctgc cgtgcccatt tacaaaggct
     1861 caaaacataa gcctgagtga gctggtagta ttttggcagg accagcaaaa gttggttctg
     1921 tacgagcact atttgggcac agagaaactt gatagtgtga atgccaagta cctgggccgc
     1981 acgagctttg acaggaacaa ctggactcta cgacttcaca atgttcagat caaggacatg
     2041 ggctcgtacg attgttttat acaaaaaaag ccacccacag gatcaattat cctccaacag
     2101 acattaacag agctgtcagt gatcgccaac ttcagtgaac ctgaaataaa actggatcag
     2161 aatgtaacag gaaattctgg cataaatttg acctgcatgt ctaagcaagg tcacccgaaa
     2221 cctaagaaga tgtattttct gataactaat tcaactaatg agtatggtga taacatgcag
     2281 atatcacaag ataatgtcac agaactgttc agtatctcca acagcctctc tctttcattc
     2341 ccggatggtg tgtggcatat gaccgttgtg tgtgttctgg aaacggagtc aatgaagatt
     2401 tcctccaaac ctctcaattt cactcaagag tttccatctg ctcaaacgta ttggaaggag
     2461 attacagctt cagttactgt ggccctcctc cttgtgatgc tgctcatcat tgtatgtcac
     2521 aagaagccga atcagcctag caggcccagc aacacagcct ctaagttaga gcgggatagt
     2581 aacgctgaca gagagactat caacctgaag gaacttgaac cccaaattgc ttcagcaaaa
     2641 ccaaatgcag aagcttaccc atacgatgtt ccagattacg cttaggatct acgggtggca
     2701 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
     2761 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
     2821 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
     2881 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
     2941 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
     3001 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
     3061 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
     3121 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta
     3181 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     3241 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     3301 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     3361 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     3421 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3481 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3541 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3601 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     3661 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3721 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     3781 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     3841 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     3901 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     3961 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4021 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     4081 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4141 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4201 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4261 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4321 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4381 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4441 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4501 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4561 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4621 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4681 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4741 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4801 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4861 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4921 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4981 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5041 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5101 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5161 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5221 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5341 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5401 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5461 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5521 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5581 gatagttacc
//
