LOCUS       sequence-30915-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Young NP, Jacks T
  TITLE     Tissue-specific p19Arf regulation dictates the response to oncogenic
            K-ras.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 1. 107(22):10184-9.
  PUBMED    20479239
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Jun 1. 107(22):10184-9."
COMMENT     Sequence Label: U6-shRNA pgkCre
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(91..107)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(92..108)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(124..436)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(185..205)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    495..612
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cctacccggt agaatgcggc cgcgctagcg tttaaactgt taattaagga attcttccta
       61 ggctgctgga atctcgtgaa gcgagcttat cgataccgtc gacctcgagg gtcaccgcgc
      121 gttaacaagg cttttctcca agggatattt atagtctcaa aacacacaat tactttacag
      181 ttagggtgag tttccttttg tgctgttttt taaaataata atttagtatt tgtatctctt
      241 atagaaatcc aagcctatca tgtaaaatgt agctagtatt aaaaagaaca gattatctgt
      301 cttttatcgc acattaagcc tctatagtta ctaggaaata ttatatgcaa attaaccggg
      361 gcaggggagt agccgagctt ctcccacaag tctgtgcgag ggggccggcg cgggcctaga
      421 gatggcgcgt cggatctcta gagcgggccc ggtactaacc aaactggatc tctgctgtcc
      481 ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt
      541 atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct
      601 tttcttttaa aattgtggat gaatactgcc atttgtctgc agaattggcg cacgcagtgc
      661 cgatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat
      721 tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga
      781 atat
//
