LOCUS       sequence-30917-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Albeck JG, Burke JM, Spencer SL, Lauffenburger DA, Sorger PK
  TITLE     Modeling a snap-action, variable-delay switch controlling extrinsic 
            cell death.
  JOURNAL   PLoS Biol. 2008 Dec 2. 6(12):2831-52.
  PUBMED    19053173
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2008 Dec 2. 6(12):2831-52."
COMMENT     Sequence Label: pECFP-IETD2x-Venus
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(154..867,868..870,871..873))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(199..220)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     548..567
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     807..828
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agctgcataa cagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
       61 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
      121 cagttatcta gaggctcgag aggccttgaa ttcttacttg tacagctcgt ccatgccgag
      181 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
      241 gtctttgctc agggcggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc
      301 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgccgc cgtcctcgat
      361 gttgtggcgg atcttgaagt tggccttgat gccgttcttc tgcttgtcgg cggtgatata
      421 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
      481 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      541 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      601 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa
      661 gcactgcagg ccgtagccca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      721 ggtggtgcag atcagcttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      781 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      841 cccggtgaac agctcctcgc ccttgctcac catggtggat ccgctgccgc cgtcggtctc
      901 gatgccgccg tcggtctcga tgccgccaga
//
