LOCUS       sequence-30918-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Albeck JG, Burke JM, Spencer SL, Lauffenburger DA, Sorger PK
  TITLE     Modeling a snap-action, variable-delay switch controlling extrinsic 
            cell death.
  JOURNAL   PLoS Biol. 2008 Dec 2. 6(12):2831-52.
  PUBMED    19053173
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2008 Dec 2. 6(12):2831-52."
COMMENT     Sequence Label: pECFP-DEVDR-Venus
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(153..866,867..869,870..872))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(198..219)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     547..566
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     806..827
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gctgcataac agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
       61 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
      121 agttatctag aggctcgaga ggccttgaat tcttacttgt acagctcgtc catgccgaga
      181 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      241 tctttgctca gggcggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg
      301 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgccgcc gtcctcgatg
      361 ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc ggtgatatag
      421 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      481 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      541 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
      601 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag
      661 cactgcaggc cgtagcccag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
      721 gtggtgcaga tcagcttcag ggtcagcttg ccgtaagtgg catcgccctc gccctcgccg
      781 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
      841 ccggtgaaca gctcctcgcc cttgctcacc atggtggatc cgctgccgta cactctgtcc
      901 acctcgtcgc cgctagat
//
