LOCUS       sequence-309228-        7041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optogenetic tool for blue-light inhibition and red-light excitation 
            of neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Vierock J, Rodriguez-Rozada S, Dieter A, Pieper F, Sims R, Tenedini 
            F, Bergs ACF, Bendifallah I, Zhou F, Zeitzschel N, Ahlbeck J, 
            Augustin S, Sauter K, Papagiakoumou E, Gottschalk A, Soba P, 
            Emiliani V, Engel AK, Hegemann P, Wiegert JS
  TITLE     BiPOLES is an optogenetic tool developed for bidirectional 
            dual-color control of neurons.
  JOURNAL   Nat Commun. 2021 Jul 26;12(1):4527. doi: 10.1038/s41467-021-24759-5.
  PUBMED    34312384
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-26"; volume: "12(1):4527. doi"; pages: " 
            10.1038/s41467-021-24759-5"
COMMENT     Sequence Label: CaMKII-somBiPOLES-mCerulean
FEATURES             Location/Qualifiers
     source          1..7041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     complement(1527..1548)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1788..1807)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2135..2156
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2556..3599
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=Chrimson
                     /translation="ELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTEWW
                     CDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSIAI
                     ALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRYFE
                     WLLSCPVILIKLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIVSC
                     IYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLKLS
                     PYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEEFV
                     EEEDEDTV"
     misc_feature    3816..4219
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     polyA_signal    4226..4274
                     /note="synthetic polyadenylation signal"
     repeat_region   4304..4444
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4304..4433
                     /label=AAV2 ITR
     rep_origin      4519..4974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4606..4625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4816..4837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4991..5010)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5110..5132
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5170..5188)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5256..5360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5361..5429,5430..6221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5579..5598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6392..6980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6881..6900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcttgtg gactaagttt gttcgcatcc ccttctccaa
      181 ccccctcagt acatcaccct gggggaacag ggtccacttg ctcctgggcc cacacagtcc
      241 tgcagtattg tgtatataag gccagggcaa agaggagcag gttttaaagt gaaaggcagg
      301 caggtgttgg ggaggcagtt accggggcaa cgggaacagg gcgtttcgga ggtggttgcc
      361 atggggacct ggatgctgac gaaggctcgc gaggctgtga gcagccacag tgccctgctc
      421 agaagcccca agctcgtcag tcaagccggt tctccgtttg cactcaggag cacgggcagg
      481 cgagtggccc ctagttctgg gggcagctct agagcggtac cggatccatg gcatcacagg
      541 tcgtctatgg ggaatgggca tcaactcaca ccgagtgcta caatatgtcc agaatcgact
      601 ccaccttcgt ctcactgctg cagctggtgt gggccgtggt ctcaggctgc cagacaatct
      661 tcatgattag cagggctccc aaggtccctt gggagtccgt gtacctgccc tttgtcgaat
      721 ctatcactta tgcactggcc agtactggca acggaaccct gcagatgagg gacggcagat
      781 tctttccttg gagccgaatg gcctcctggc tgtgcacctg tccaatcatg ctgggacaga
      841 tttctaacat ggctctggtg aagtacaaaa gtatcccact gaatcccatt gcacaggccg
      901 cttccatcat tagagtggtc atgggaatca ccgctacaat ttctcccgca gagtatatga
      961 agtggctgtt ctttttcttt ggggccacct gcctggtgtt cgagtacagt gtggtcttca
     1021 caatctttca agtggggctg tatggctttg aatctgtcgg gacacctctg gcacagaagg
     1081 tggtggtgag gatcaagatg ctgcgcctga tcttctttat tgcttggact atgttcccaa
     1141 tcgtgtggct gatttcaccc accggagtct gcgtgatcca cgagaacgtg agcgccattc
     1201 tgtacctgct ggctgacggc ctgtgcaaaa atacatatgg agtgatcctg tggagcactg
     1261 cctggggagt gctggaaggg aagtgggacc ccgcatgtct gccagggcag gagaaacccg
     1321 aagccgacga tccttttggc ctgaatcacg agaagaatgc ccctcctaac gacgaagtca
     1381 atatcagaat gtttggaagg ggacgtacgg cggccaagag caggatcacc agcgagggcg
     1441 agtacatccc cctggaccag atcgacatca acgtggcggt acccgtgagc aagggcgagg
     1501 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1561 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1621 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgagct
     1681 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1741 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1801 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1861 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca
     1921 tccacggcaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttcg
     1981 gcctcaactg caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2041 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca
     2101 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2161 ccgccgggat cactctcggc atggacgagc tgtataccgg tgcagccctg caggagaaga
     2221 agtcatgcag ccagcgcatg gccgaattcc ggcaatactg ttggaacccg gacactgggc
     2281 agatgctggg ccgcacccca gcccggtggg tgtggatcag cctgtactat gcagctttct
     2341 acgtggtcat gactgggctc tttgccttgt gcatctatgt gctgatgcag accattgatc
     2401 cctacacccc cgactaccag gaccagttaa agtcaccggg ggtaaccttg agaccggatg
     2461 tgtatgggga aagagggctg cagatttcct acaacatctc tgaaaacagc tctagacagg
     2521 cccagatcac cggacgtccg gagggagctg tacaagagct gatcagcagc gccaccagat
     2581 ctctgtttgc cgccggaggc atcaaccctt ggcctaaccc ctaccaccac gaggacatgg
     2641 gctgtggagg aatgacacct acaggcgagt gcttcagcac cgagtggtgg tgtgaccctt
     2701 cttacggact gagcgacgcc ggatacggat attgcttcgt ggaggccaca ggcggctacc
     2761 tggtcgtggg agtggagaag aagcaggctt ggctgcacag cagaggcaca ccaggagaaa
     2821 agatcggcgc ccaggtctgc cagtggattg ctttcagcat cgccatcgcc ctgctgacat
     2881 tctacggctt cagcgcttgg aaggccactt gcggttggga ggaggtctac gtctgttgcg
     2941 tcgaggtgct gttcgtgacc ctggagatct tcaaggagtt cagcagcccc gccacagtgt
     3001 acctgtctac cggcaaccac gcctattgcc tgcgctactt cgagtggctg ctgtcttgcc
     3061 ccgtgatcct gatcaagctg agcaacctga gcggcctgaa gaacgactac agcaagcgga
     3121 ccatgggcct gatcgtgtct tgcgtgggaa tgatcgtgtt cggcatggcc gcaggactgg
     3181 ctaccgattg gctcaagtgg ctgctgtata tcgtgtcttg catctacggc ggctacatgt
     3241 acttccaggc cgccaagtgc tacgtggaag ccaaccacag cgtgcctaaa ggccattgcc
     3301 gcatggtcgt gaagctgatg gcctacgctt acttcgcctc ttggggcagc tacccaatcc
     3361 tctgggcagt gggaccagaa ggactgctga agctgagccc ttacgccaac agcatcggcc
     3421 acagcatctg cgacatcatc gccaaggagt tttggacctt cctggcccac cacctgagga
     3481 tcaagatcca cgagcacatc ctgatccacg gcgacatccg gaagaccacc aagatggaga
     3541 tcggaggcga ggaggtggaa gtggaagagt tcgtggagga ggaggacgag gacacagtgc
     3601 tcgagcagag ccagcccatc ctgaacacca aggagatggc cccccagagc aagccccccg
     3661 aggagctgga gatgagcagc atgcccagcc ccgtggcccc cctgcccgcc cgcaccgagg
     3721 gcgtgatcga catgcgcagc atgagcagca tcgacagctt catcagctgc gccaccgact
     3781 tccccgaggc cacccgcttc tgatctagaa agcttaatca acctctggat tacaaaattt
     3841 gtgaaagatt gactgatatt cttaactatg ttgctccttt tacgctgtgt ggatatgctg
     3901 ctttaatgcc tctgtatcat gctattgctt cccgtatggc tttcgttttc tcctccttgt
     3961 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtccgt caacgtggcg
     4021 tggtgtgctc tgtgtttgct gacgcaaccc ccactggctg gggcattgcc accacctgtc
     4081 aactcctttc tgggactttc gctttccccc tcccgatcgc cacggcagaa ctcatcgccg
     4141 cctgccttgc ccgctgctgg acaggggcta ggttgctggg cactgataat tccgtggtgt
     4201 tgtcggggaa gctgacgtca gcgctaataa aatcgctatc catcgaagat ggatgtgtgt
     4261 tggttttttg tgtgggtaac ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     4321 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     4381 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     4441 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4501 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4561 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     4621 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4681 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4741 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4801 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     4861 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     4921 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     4981 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     5041 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     5101 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     5161 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5221 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5281 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5341 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5401 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5461 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5521 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5581 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5641 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5701 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5761 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5821 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5881 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5941 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6001 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6061 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6121 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6181 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6241 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6301 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6361 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6421 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6481 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6541 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6601 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6661 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6721 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6781 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6901 atagtcctgt cgggttttgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7021 gctggccttt tgctcacatg t
//
