LOCUS       sequence-309306-        6128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase under the control of the Drosophila melanogaster 
            minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A 
            NF-kappa-B site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K
  TITLE     Imd pathway-specific immune assays reveal NF-kappaB stimulation by 
            viral RNA PAMPs in Aedes aegypti Aag2 cells.
  JOURNAL   PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 
            10.1371/journal.pntd.0008524.
  PUBMED    33591970
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2021-02-16"; volume: "15(2):e0008524. doi"; 
            pages: " 10.1371/journal.pntd.0008524"
COMMENT     Sequence Label: pKM51
FEATURES             Location/Qualifiers
     source          1..6128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(137..205,206..997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(355..374)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1168..1756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1657..1676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1910..1927
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1942..2082
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2008..2027)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    2321..2455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2549..2683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             3244..4896
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3310..3328)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          5027..5092
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5222..5242
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5667..5801
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6074..6092)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1561 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1801 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1861 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1921 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     1981 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2041 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2101 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2161 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     2221 caggatcagc catatcacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     2281 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt aacttgttta ttgcagctta
     2341 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2401 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcagcca
     2461 tatcacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     2521 gaaacataaa atgaatgcaa ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2581 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2641 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccaagctt cccgggcaga
     2701 tctctcgagg tcgacccggg gatctcgaga cgcgggaaat tccaacgtcg agacgcggga
     2761 aattccaacg tcgagacgcg ggaaattcca acgtcgagac gcgggaaatt ccaacgaatt
     2821 tcgagacgcg ggaaattcca acgtcgagac gcgggaaatt ccaacgtcga gacgcgggaa
     2881 attccaacgt cgagacgcgg gaaattccaa cgagcttaat tagatctaat tcgccggagt
     2941 ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac
     3001 gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca
     3061 gtaaagtgca agttaaagtg aatcaattaa aagtaaccag caaccaagta aatcaactgc
     3121 aactactgag atctgccaag aagtaattat tgaatacaat ataaagcata taaacagccc
     3181 agcttggagc gagtgaccat gggagctcga attccagctt ggcattccgg tactgttggt
     3241 aaaatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc tctagaggat
     3301 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
     3361 attgctttta cagatgcaca tatcgaggtg aacatcacgt acgcggaata cttcgaaatg
     3421 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
     3481 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
     3541 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgaac
     3601 atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
     3661 caaaaaaaat taccaataat ccagaaaatt attatcatgg attctaaaac ggattaccag
     3721 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
     3781 gattttgtac cagagtcctt tgatcgtgac aaaacaattg cactgataat gaattcctct
     3841 ggatctactg ggttacctaa gggtgtggcc cttccgcata gaactgcctg cgtcagattc
     3901 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
     3961 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     4021 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttttacgatc ccttcaggat
     4081 tacaaaattc aaagtgcgtt gctagtacca accctatttt cattcttcgc caaaagcact
     4141 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggggg cgcacctctt
     4201 tcgaaagaag tcggggaagc ggttgcaaaa cgcttccatc ttccagggat acgacaagga
     4261 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     4321 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     4381 aaaacgctgg gcgttaatca gagaggcgaa ttatgtgtca gaggacctat gattatgtcc
     4441 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     4501 tctggagaca tagcttactg ggacgaagac gaacacttct tcatagttga ccgcttgaag
     4561 tctttaatta aatacaaagg atatcaggtg gcccccgctg aattggaatc gatattgtta
     4621 caacacccca acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga cgccggtgaa
     4681 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     4741 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     4801 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
     4861 ataaaggcca agaagggcgg aaagtccaaa ttgtaaaatg taactgtatt cagcgatgac
     4921 gaaattctta gctattgtaa tactctagag gatctttgtg aaggaacctt acttctgtgg
     4981 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     5041 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     5101 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     5161 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     5221 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     5281 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     5341 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     5401 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     5461 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     5521 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     5581 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     5641 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     5701 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5761 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccccaggaag ctcctctgtg
     5821 tcctcataaa ccctaacctc ctctacttga gaggacattc caatcatagg ctgcccatcc
     5881 accctctgtg tcctcctgtt aattaggtca cttaacaaaa aggaaattgg gtaggggttt
     5941 ttcacagacc gctttctaag ggtaatttta aaatatctgg gaagtccctt ccactgctgt
     6001 gttccagaag tgttggtaaa cagcccacaa atgtcaacag cagaaacata caagctgtca
     6061 gctttgcaca agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     6121 gtttctta
//
