LOCUS       sequence-309312-        5900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase under the control of the Drosophila melanogaster 
            minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 
            NF-kappa-B site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K
  TITLE     Imd pathway-specific immune assays reveal NF-kappaB stimulation by 
            viral RNA PAMPs in Aedes aegypti Aag2 cells.
  JOURNAL   PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 
            10.1371/journal.pntd.0008524.
  PUBMED    33591970
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2021-02-16"; volume: "15(2):e0008524. doi"; 
            pages: " 10.1371/journal.pntd.0008524"
COMMENT     Sequence Label: pKM52
FEATURES             Location/Qualifiers
     source          1..5900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(137..205,206..997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(355..374)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1168..1756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1657..1676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1910..1927
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1942..2082
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2008..2027)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    2321..2455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             3016..4668
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3082..3100)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          4799..4864
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4994..5014
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5439..5573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5846..5864)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1561 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1801 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1861 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1921 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     1981 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2041 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2101 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2161 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     2221 caggatcagc catatcacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     2281 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt aacttgttta ttgcagctta
     2341 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2401 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccaagc
     2461 ttcccgggca gatctctcga ggtcgacccg gggatccacg ctttcggtga tttacgcacc
     2521 acgctttcgg tgatttacgc accacgcttt cggtgattta cgcaccacgc tttcggtgat
     2581 ttacgcacaa ttcacgcttt cggtgattta cgcaccacgc tttcggtgat ttacgcacca
     2641 cgctttcggt gatttacgca ccacgctttc ggtgatttac gcacagctta attagatcta
     2701 attcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag
     2761 caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct
     2821 aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc agcaaccaag
     2881 taaatcaact gcaactactg agatctgcca agaagtaatt attgaataca atataaagca
     2941 tataaacagc ccagcttgga gcgagtgacc atgggagctc gaattccagc ttggcattcc
     3001 ggtactgttg gtaaaatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     3061 cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     3121 gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac gtacgcggaa
     3181 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     3241 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     3301 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     3361 aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa
     3421 attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat ggattctaaa
     3481 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     3541 tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat tgcactgata
     3601 atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca tagaactgcc
     3661 tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     3721 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     3781 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttttacga
     3841 tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt ttcattcttc
     3901 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggg
     3961 ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca tcttccaggg
     4021 atacgacaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     4081 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     4141 ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt cagaggacct
     4201 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     4261 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatagtt
     4321 gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc tgaattggaa
     4381 tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct tcccgacgat
     4441 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     4501 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     4561 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     4621 agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaaaa tgtaactgta
     4681 ttcagcgatg acgaaattct tagctattgt aatactctag aggatctttg tgaaggaacc
     4741 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     4801 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     4861 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     4921 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     4981 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     5041 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     5101 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     5161 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     5221 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     5281 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     5341 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5401 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5461 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5521 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccccagga
     5581 agctcctctg tgtcctcata aaccctaacc tcctctactt gagaggacat tccaatcata
     5641 ggctgcccat ccaccctctg tgtcctcctg ttaattaggt cacttaacaa aaaggaaatt
     5701 gggtaggggt ttttcacaga ccgctttcta agggtaattt taaaatatct gggaagtccc
     5761 ttccactgct gtgttccaga agtgttggta aacagcccac aaatgtcaac agcagaaaca
     5821 tacaagctgt cagctttgca caagggcctc gtgatacgcc tatttttata ggttaatgtc
     5881 atgataataa tggtttctta
//
