LOCUS       sequence-309332-        6060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase under the control of the enhancer for the 
            Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak 
            sequence of the Metchnikowin gene and additional NF-kappa-B site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K
  TITLE     Imd pathway-specific immune assays reveal NF-kappaB stimulation by 
            viral RNA PAMPs in Aedes aegypti Aag2 cells.
  JOURNAL   PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 
            10.1371/journal.pntd.0008524.
  PUBMED    33591970
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2021-02-16"; volume: "15(2):e0008524. doi"; 
            pages: " 10.1371/journal.pntd.0008524"
COMMENT     Sequence Label: pKM59
FEATURES             Location/Qualifiers
     source          1..6060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1911..2051
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1977..1996)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    2290..2424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2518..2652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             3145..4797
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3211..3229)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          4928..4993
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5123..5143
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5568..5702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5975..5993)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     1921 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     1981 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     2041 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2101 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2161 tttcaccgtc atcaccgaaa cgcgcgaggc aggatcagcc atatcacatt tgtagaggtt
     2221 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     2281 attgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2341 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2401 aatgtatctt atcatgtctg gatcagccat atcacatttg tagaggtttt acttgcttta
     2461 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttaac
     2521 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2581 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     2641 catgtctgga tccaagcttg cccgggcaga tctctcgagg cgtggctccg tgttgacgca
     2701 tgttgactat gcctttgaat ggctgccgtg gttgtcggtg ggtaatttgc aatgcagaaa
     2761 aaccaacagg gcgctaaaaa ggagagtgtt ttcgtgggag gtggagatgg tcactggggg
     2821 caacataaat attcagcgag aaacgtcata tttacattta gtctaggctg ataatccggg
     2881 accgtgggaa gtcccctttg ggtggtgctg gctgggttcc cctggccaca atcggttatc
     2941 tgcccccggc tgacacttgc ccgtcattca ttcggctgct tatcgcagaa gctcaaataa
     3001 aaagtcccca atctgcgact cgtttgtctg ggactgagct ataaaagcct caccatctca
     3061 acgctcaaag catcaatcaa ttcccgccac cgagctaaga tgggagctcg aattccagct
     3121 tggcattccg gtactgttgg taaaatggaa gacgccaaaa acataaagaa aggcccggcg
     3181 ccattctatc ctctagagga tggaaccgct ggagagcaac tgcataaggc tatgaagaga
     3241 tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt gaacatcacg
     3301 tacgcggaat acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg
     3361 aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg
     3421 ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt
     3481 gaattgctca acagtatgaa catttcgcag cctaccgtag tgtttgtttc caaaaagggg
     3541 ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg
     3601 gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta
     3661 cctcccggtt ttaatgaata cgattttgta ccagagtcct ttgatcgtga caaaacaatt
     3721 gcactgataa tgaattcctc tggatctact gggttaccta agggtgtggc ccttccgcat
     3781 agaactgcct gcgtcagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt
     3841 ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca
     3901 ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg
     3961 tttttacgat cccttcagga ttacaaaatt caaagtgcgt tgctagtacc aaccctattt
     4021 tcattcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt
     4081 gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag cggttgcaaa acgcttccat
     4141 cttccaggga tacgacaagg atatgggctc actgagacta catcagctat tctgattaca
     4201 cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag
     4261 gttgtggatc tggataccgg gaaaacgctg ggcgttaatc agagaggcga attatgtgtc
     4321 agaggaccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt
     4381 gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc
     4441 ttcatagttg accgcttgaa gtctttaatt aaatacaaag gatatcaggt ggcccccgct
     4501 gaattggaat cgatattgtt acaacacccc aacatcttcg acgcgggcgt ggcaggtctt
     4561 cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg
     4621 atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg
     4681 cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca
     4741 agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagtccaa attgtaaaat
     4801 gtaactgtat tcagcgatga cgaaattctt agctattgta atactctaga ggatctttgt
     4861 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     4921 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     4981 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
     5041 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
     5101 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
     5161 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
     5221 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
     5281 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
     5341 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
     5401 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
     5461 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     5521 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     5581 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5641 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     5701 tccccaggaa gctcctctgt gtcctcataa accctaacct cctctacttg agaggacatt
     5761 ccaatcatag gctgcccatc caccctctgt gtcctcctgt taattaggtc acttaacaaa
     5821 aaggaaattg ggtaggggtt tttcacagac cgctttctaa gggtaatttt aaaatatctg
     5881 ggaagtccct tccactgctg tgttccagaa gtgttggtaa acagcccaca aatgtcaaca
     5941 gcagaaacat acaagctgtc agctttgcac aagggcctcg tgatacgcct atttttatag
     6001 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
//
