LOCUS       sequence-309339-        4155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EcoRV digestion produces 12x601 chromatin assembly construct with 30
            bp linker lengths in addition to carrier DNA that prevents 
            overassembly of nucleosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pWM_12x601_30bpLinker
FEATURES             Location/Qualifiers
     source          1..4155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    25..41
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     30..52
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     49..65
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     49..65
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2451..2542
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3478..4065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3966..3985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4106..4127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4142..4147,4148..4155,1..10,11..17)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tgccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
       61 atgactatga ttacgcccaa gcttgatatc atgaggatgg gaacaggatg tatatatctg
      121 acacgtgcct ggagactagg gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg
      181 tacgtgcgtt taagcggtgc tagagctgtc tacgaccaat tgagcggcct cggcaccggg
      241 attctccagt tacatgatcc atctagatgg aggatgggaa caggatgtat atatctgaca
      301 cgtgcctgga gactagggag taatcccctt ggcggttaaa acgcggggga cagcgcgtac
      361 gtgcgtttaa gcggtgctag agctgtctac gaccaattga gcggcctcgg caccgggatt
      421 ctccagttac atgatccagc tagatggagg atgggaacag gatgtatata tctgacacgt
      481 gcctggagac tagggagtaa tccccttggc ggttaaaacg cgggggacag cgcgtacgtg
      541 cgtttaagcg gtgctagagc tgtctacgac caattgagcg gcctcggcac cgggattctc
      601 cagttacatg atccagctag atggaggatg ggaacaggat gtatatatct gacacgtgcc
      661 tggagactag ggagtaatcc ccttggcggt taaaacgcgg gggacagcgc gtacgtgcgt
      721 ttaagcggtg ctagagctgt ctacgaccaa ttgagcggcc tcggcaccgg gattctccag
      781 ttacatgatc cagctagatg gaggatggga acaggatgta tatatctgac acgtgcctgg
      841 agactaggga gtaatcccct tggcggttaa aacgcggggg acagcgcgta cgtgcgttta
      901 agcggtgcta gagctgtcta cgaccaattg agcggcctcg gcaccgggat tctccagtta
      961 catgatccag ctagatggag gatgggaaca ggatgtatat atctgacacg tgcctggaga
     1021 ctagggagta atccccttgg cggttaaaac gcgggggaca gcgcgtacgt gcgtttaagc
     1081 ggtgctagag ctgtctacga ccaattgagc ggcctcggca ccgggattct ccagttacat
     1141 gatccagcta gatggaggat gggaacagga tgtatatatc tgacacgtgc ctggagacta
     1201 gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt
     1261 gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca gttacatgat
     1321 ccagctagat ggaggatggg aacaggatgt atatatctga cacgtgcctg gagactaggg
     1381 agtaatcccc ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt aagcggtgct
     1441 agagctgtct acgaccaatt gagcggcctc ggcaccggga ttctccagtt acatgatcca
     1501 gctagatgga ggatgggaac aggatgtata tatctgacac gtgcctggag actagggagt
     1561 aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag cggtgctaga
     1621 gctgtctacg accaattgag cggcctcggc accgggattc tccagttaca tgatccagct
     1681 agatggagga tgggaacagg atgtatatat ctgacacgtg cctggagact agggagtaat
     1741 ccccttggcg gttaaaacgc gggggacagc gcgtacgtgc gtttaagcgg tgctagagct
     1801 gtctacgacc aattgagcgg cctcggcacc gggattctcc agttacatga tccagctaga
     1861 tggaggatgg gaacaggatg tatatatctg acacgtgcct ggagactagg gagtaatccc
     1921 cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc tagagctgtc
     1981 tacgaccaat tgagcggcct cggcaccggg attctccagt tacatgatcc agctagctgg
     2041 aggatgggaa caggatgtat atatctgaca cgtgcctgga gactagggag taatcccctt
     2101 ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac
     2161 gaccaattga gcggcctcgg caccgggatt ctccagttac atgatcatga tatcggatcc
     2221 ccgggtaccg agctcgaatt cgggcttgcc gtcgttttac agcgacgaga ctgggaaaat
     2281 cctggcgtta cccaacttaa tcgccttgcc gcacaccccc ctttcgccag ttggcgtaat
     2341 agcgaagaag cccgcactga ccgcccttcc caacagttgc gtagtctgaa tggcgaatgg
     2401 cgttaaacta gtagcccgcc taatgagcgg gctttttttt aattccccta tttgtttatt
     2461 tttctaaata cattcaaata gatatccgct catgagacaa taaccctgat aaatgcttca
     2521 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2581 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctcgtga aagtaaaaga
     2641 cgcagaggac caattggggg cacgagtggg atacatagaa ctggacttga atagcggtaa
     2701 aatccttgag agttttcgcc ctgaagagcg ttttccaatg atgagcactt tcaaagttct
     2761 gctatgtgga gcagtattat cccgtgtaga tgcggggcaa gagcaactcg gacgacggat
     2821 atcctattcg cagaatgact tggttgaata ctccccagtg acagaaaagc accttacgga
     2881 cggaatgacg gtaagagaat tatgtagtgc cgccataacg atgagtgata acactgcggc
     2941 gaacttactt ctgacaacca tcggtggacc gaaggaatta accgcttttt tgcacaatat
     3001 gggagaccat gtaactcgcc ttgaccgttg ggaaccagaa ctgaatgaag ccataccaaa
     3061 cgacgagcga gacaccacaa tgcctgcggc aatggcaaca acattacgca aactgatatc
     3121 tggcgaacta cttactctgg cttcacggca acaattaata gactggcttg aagcggataa
     3181 agttgcagga ccactactgc gttcggcact tcctgctggc tggtttattg ctgataaatc
     3241 tggggcagga gagcgtggtt cacggggtat cattgccgca cttggaccag atggtaagcc
     3301 ttcccgtatc gtagttatct acacgacggg tagtcaggca actatggacg aacgaaatag
     3361 acagattgct gaaatagggg cttcactgat taagcattgg taaaccgata tcattaaagg
     3421 ctccttttgg agcctttttt tttggacgga ccgagtagaa aagatcaaag gatcttcttg
     3481 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3541 ggtggtatgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3601 cagagcgcag ataccaaata ctgttcttct agtgtagccg gatatcggcc accacttcaa
     3661 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3721 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3781 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3841 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgttcc gatatcgaga
     3901 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3961 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4021 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4081 gcctttttac ggttcctgaa ttgattaatg tgagttagct cactcattag gcaccccagg
     4141 ctttacactt tatgc
//
