LOCUS       sequence-309343-        6059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase under the control of the enhancer for the 
            Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific 
            (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response 
            elements, plus an additional NF-_B site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K
  TITLE     Imd pathway-specific immune assays reveal NF-kappaB stimulation by 
            viral RNA PAMPs in Aedes aegypti Aag2 cells.
  JOURNAL   PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 
            10.1371/journal.pntd.0008524.
  PUBMED    33591970
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2021-02-16"; volume: "15(2):e0008524. doi"; 
            pages: " 10.1371/journal.pntd.0008524"
COMMENT     Sequence Label: pKM62
FEATURES             Location/Qualifiers
     source          1..6059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    95..229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             721..2373
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(787..805)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2504..2569
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2699..2719
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3144..3278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3551..3569)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3637..3741
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3742..3810,3811..4602)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3960..3979)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4773..5361
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5262..5281
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5515..5532
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5547..5687
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5613..5632)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    join(5926..6059,1)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cagccatatc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
       61 gaacctgaaa cataaaatga atgcaattgt tgttaacttg tttattgcag cttataatgg
      121 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      181 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc aagcttgccc
      241 gggcagatct ctcgaggcgt ggctccgtgt tgacgcatgt tgactatgcc tttgaatggc
      301 tgccgtcacg ctttcggtga tttacgcact gcagaaaaac caacagggcg ctaaaaagga
      361 gagtgttttc gtgggaggtg gagatggtca ctgggggcaa cataaatatt cagcgagaaa
      421 cgtcatattt acatttagtc taggctgata atccgggctt tcgggattta cgcacgggtg
      481 gtgcgtgcgt aaatcaccga aagcgtgtcg gttatctgcc cccggctgac acttgcccgt
      541 cattcattcg gctgcttatc gcagaagctc gtgcgtaaat caccgaaagc gtgactcgtt
      601 tgtctgggac tgagctataa aagcctcacc atctcaacgc tcaaagcgtg cgtaaatcac
      661 cgaaagcgtg ctaagatggg agctcgaatt ccagcttggc attccggtac tgttggtaaa
      721 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga
      781 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      841 gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc
      901 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      961 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
     1021 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt
     1081 tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
     1141 aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga
     1201 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
     1261 tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga
     1321 tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg
     1381 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
     1441 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
     1501 cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac
     1561 aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg
     1621 attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg
     1681 aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat
     1741 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     1801 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     1861 acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt
     1921 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     1981 ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct
     2041 ttaattaaat acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa
     2101 caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt
     2161 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     2221 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     2281 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     2341 aaggccaaga agggcggaaa gtccaaattg taaaatgtaa ctgtattcag cgatgacgaa
     2401 attcttagct attgtaatac tctagaggat ctttgtgaag gaaccttact tctgtggtgt
     2461 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     2521 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     2581 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     2641 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     2701 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     2761 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     2821 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
     2881 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
     2941 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
     3001 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     3061 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     3121 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3181 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3241 tgtccaaact catcaatgta tcttatcatg tctggatccc caggaagctc ctctgtgtcc
     3301 tcataaaccc taacctcctc tacttgagag gacattccaa tcataggctg cccatccacc
     3361 ctctgtgtcc tcctgttaat taggtcactt aacaaaaagg aaattgggta ggggtttttc
     3421 acagaccgct ttctaagggt aattttaaaa tatctgggaa gtcccttcca ctgctgtgtt
     3481 ccagaagtgt tggtaaacag cccacaaatg tcaacagcag aaacatacaa gctgtcagct
     3541 ttgcacaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     3601 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3661 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3721 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3781 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3841 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3901 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3961 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4021 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4081 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4141 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4201 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4261 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4321 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4381 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4441 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4501 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4561 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4621 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4681 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4741 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4801 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4861 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4921 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4981 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5041 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5101 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5161 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5221 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5281 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5341 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5401 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5461 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5521 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     5581 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     5641 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     5701 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     5761 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     5821 cgaggcagga tcagccatat cacatttgta gaggttttac ttgctttaaa aaacctccca
     5881 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttaactt gtttattgca
     5941 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6001 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggat
//
